Expression of the functional carbohydrate-binding module (CBM) of human laforin

Protein Expression and Purification

Volumn 74, Issue 2, 2010, Pages 169-174

  Moreira, Susana ^a   Castanheira, Pedro ^b   Casal, Margarida ^a   Faro, Carlos ^b   Gama, Miguel ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b Biocant Molecular Biotechnology Unit Parque Tecnológico de Cantanhede   (Portugal)

Author keywords

Carbohydrate binding module; Inclusion bodies; Laforin; RGD

Indexed keywords

EPM2A PROTEIN, HUMAN; NON RECEPTOR PROTEIN TYROSINE PHOSPHATASE; RECOMBINANT PROTEIN;

ARTICLE; CARBOHYDRATE METABOLISM; CELL INCLUSION; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE;

CARBOHYDRATE METABOLISM; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN TYROSINE PHOSPHATASES, NON-RECEPTOR; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 77957748954     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.06.019     Document Type: Article

References (40)

1. S. Ganesh, K.L. Agarwala, K. Ueda, T. Akagi, K. Shoda, T. Usui, T. Hashikawa, H. Osada, A.V. Delgado-Escueta, and K. Yamakawa Laforin, defective in the progressive myoclonus epilepsy of Lafora type, is a dual-specificity phosphatase associated with polyribosomes Hum. Mol. Genet. 9 2000 2251 2261
2. J. Wang, J.A. Stuckey, M.J. Wishar, and J.E. Dixon A unique carbohydrate binding domain targets the Lafora disease phosphatase to glycogen J. Biol. Chem. 277 2002 2377 2380
3. W. Wang, and P.J. Roach Glycogen and related polysaccharides inhibit the laforin dual-specificity protein phosphatase Biochem. Biophys. Res. Commun. 325 2004 726 730
4. B.L. Cantarel, P.M. Coutinho, C. Rancurel, T. Bernard, V. Lombard, and B. Henrissat The carbohydrate-active enZymes database (CAZy): an expert resource for glycogenomics Nucleic Acids Res. 37 2009 D233 238
5. O. Shoseyov, Z. Shani, and I. Levy Carbohydrate binding modules: biochemical properties and novel applications Microbiol. Mol. Biol. Rev. 70 2006 283 295
6. J.-M. Girard, K.H.D. Lê, and F. Lederer Molecular characterization of laforin, a dual-specificity protein phosphatase implicated in Lafora disease Biochimie 88 2006 1961 1971
7. C.A. Worby, M.S. Gentry, and J.E. Dixon Laforin, a dual specificity phosphatase that dephosphorylates complex carbohydrates J. Biol. Chem. 281 2006 30412 30418
8. E.M. Chan, C.A. Ackerley, H. Lohi, L. Ianzano, M.A. Cortez, P. Shannon, S.W. Scherer, and B.A. Minassian Laforin preferentially binds the neurotoxic starch-like polyglucosans, which form in its absence in progressive myoclonus epilepsy Hum. Mol. Genet. 13 2004 1117 1129
9. D.S. Hwang, S.B. Sim, and H.J. Cha Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide Biomaterials 28 2007 4039 4046
10. W. Dai, and W.M. Saltzman Fibroblast aggregation by suspension with conjugates of poly(ethylene glycol) and RGD Biotechnol. Bioeng. 50 1996 349 356
11. B.K. Mann, and J.L. West Cell adhesion peptides alter smooth muscle cell adhesion, proliferation, migration, and matrix protein synthesis on modified surfaces and in polymer scaffolds J. Biomed. Mater. Res. 60 2002 86 93
12. A.J. Schraa, R.J. Kok, A.D. Berendsen, H.E. Moorlag, E.J. Bos, D.K. Meijer, L.F. de Leij, and G. Molema Endothelial cells internalize and degrade RGD-modified proteins developed for tumor vasculature targeting J. Controlled Release 83 2002 241 251
13. J. Tan, R.A. Gemeinhart, M. Ma, and W.M. Saltzman Improved cell adhesion and proliferation on synthetic phosphonic acid-containing hydrogels Biomaterials 26 2005 3663 3671
14. E. Ruoslahti, and M.D. Pierschbacher New perspectives in cell adhesion: RGD and integrins Science 238 1987 491 497
15. J. Li, M. Ding, Q. Fu, H. Tan, X. Xie, and Y. Zhong A novel strategy to graft RGD peptide on biomaterials surfaces for endothelization of small-diameter vascular grafts and tissue engineering blood vessel J. Mater. Sci. Mater. Med. 19 2008 2595 2603
16. U. Hersel, C. Dahmen, and H. Kessler RGD modified polymers: biomaterials for stimulated cell adhesion and beyond Biomaterials 24 2003 4385 4415
17. S.P. Massia, and J. Stark Immobilized RGD peptides on surface-grafted dextran promote biospecific cell attachment J. Biomed. Mater. Res. 56 2001 390 399
18. T.W. Wang, H.C. Wu, Y.C. Huang, J.S. Sun, and F.H. Lin The effect of self-designed bifunctional RGD-containing fusion protein on the behavior of human keratinocytes and dermal fibroblasts J. Biomed. Mater. Res. B. Appl. Biomater. 79 2006 379 387
19. A. Wierzba, U. Reichl, R.F. Turner, R.A. Warren, and D.G. Kilburn Adhesion of mammalian cells to a recombinant attachment factor, CBD/RGD, analyzed by image analysis Biotechnol. Bioeng. 46 1995 185 193
20. F.K. Andrade, S.M. Moreira, L. Domingues, and F.M. Gama Improving the affinity of fibroblasts for bacterial cellulose using carbohydrate-binding modules fused to RGD J. Biomed. Mater. Res. A 2009
21. C.I. Guerreiro, C.M. Fontes, M. Gama, and L. Domingues Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum Protein Expr. Purif. 59 2008 161 168
22. S.M. Moreira, F.K. Andrade, L. Domingues, and M. Gama Development of a strategy to functionalize a dextrin-based hydrogel for animal cell cultures using a starch-binding module fused to RGD sequence BMC Biotechnol. 8 2008 78
23. F. Sanger, S. Nicklen, and A.R. Coulson DNA sequencing with chain-terminating inhibitors Proc. Natl. Acad. Sci. USA 74 1977 5463 5467
24. G.E. Sroga, and J. Dordick A strategy for in vivo screening of subtilisin E reaction specificity in E Coli periplasm Biotechnol. Bioeng. 78 2002 761 769
25. Y.K. Chae, H. Im, Q. Zhao, J.H. Doelling, R.D. Vierstra, and J.L. Markley Prevention of aggregation after refolding by balanced stabilization- destabilization: production of the Arabidopsis thaliana protein APG8a (At4g21980) for NMR structure determination Protein Expr. Purif. 34 2004 280 283
26. Y. Liu, Y. Wang, C. Wu, and P. Zheng Dimerization of laforin is required for its optimal phosphatase activity, regulation of GSK3beta phosphorylation, and Wnt signaling J. Biol. Chem. 281 2006 34768 34774
27. M.R. Dyson, S.P. Shadbolt, K.J. Vincent, R.L. Perera, and J. McCafferty Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression BMC Biotechnol. 4 2004 32
28. H.P. Sorensen, and K.K. Mortensen Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli Microb. Cell. Fact. 4 2005 1
29. K. Terpe Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems Appl. Microbiol. Biotechnol. 72 2006 211 222
30. H.P. Sorensen, and K.K. Mortensen Advanced genetic strategies for recombinant protein expression in Escherichia coli J. Biotechnol. 115 2005 113 128
31. J.G. Thomas, and F. Baneyx Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins J. Biol. Chem. 271 1996 11141 11147
32. A. Villaverde, and M.M. Carrio Protein aggregation in recombinant bacteria: biological role of inclusion bodies Biotechnol. Lett. 25 2003 1385 1395
33. D. Esposito, and D.K. Chatterjee Enhancement of soluble protein expression through the use of fusion tags Curr. Opin. Biotechnol. 17 2006 353 358 (Pubitemid 44163452)
34. S. Jana, and J.K. Deb Strategies for efficient production of heterologous proteins in Escherichia coli Appl. Microbiol. Biotechnol. 67 2005 289 298
35. P. Braun, Y. Hu, B. Shen, A. Halleck, M. Koundinya, E. Harlow, and J. LaBaer Proteome-scale purification of human proteins from bacteria Proc. Natl. Acad. Sci. USA 99 2002 2654 2659
36. M. Hammarstrom, N. Hellgren, S. van Den Berg, H. Berglund, and T. Hard Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli Protein Sci. 11 2002 313 321
37. Y.P. Shih, W.M. Kung, J.C. Chen, C.H. Yeh, A.H. Wang, and T.F. Wang High-throughput screening of soluble recombinant proteins Protein Sci. 11 2002 1714 1719
38. M.E. Fernandez-Sanchez, O. Criado-Garcia, K.E. Heath, B. Garcia-Fojeda, I. Medrano-Fernandez, P. Gomez-Garre, P. Sanz, J.M. Serratosa, and S. Rodriguez de Cordoba Laforin, the dual-phosphatase responsible for Lafora disease, interacts with R5 (PTG), a regulatory subunit of protein phosphatase-1 that enhances glycogen accumulation Hum. Mol. Genet. 12 2003 3161 3171
39. B.A. Minassian Lafora's disease: towards a clinical, pathologic, and molecular synthesis Pediatr. Neurol. 25 2001 21 29
40. D. Dubey, and S. Ganesh Modulation of functional properties of laforin phosphatase by alternative splicing reveals a novel mechanism for the EPM2A gene in Lafora progressive myoclonus epilepsy Hum. Mol. Genet. 17 2008 3010 3020