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Volumn 22, Issue 8, 2010, Pages 1237-1246

Efficient system for preservation and regeneration of genetic resources in chicken: Concurrent storage of primordial germ cells and live animals from early embryos of a rare indigenous fowl (Gifujidori)

Author keywords

germline chimeras

Indexed keywords

LIQUID NITROGEN;

EID: 77957737110     PISSN: 10313613     EISSN: None     Source Type: Journal    
DOI: 10.1071/RD10056     Document Type: Article
Times cited : (38)

References (39)
  • 1
    • 54349105936 scopus 로고    scopus 로고
    • Restriction of germline proliferation by soft X-ray irradiation of chicken embryos and its application to chimera production
    • doi:10. 2141/JPSA. 45. 292
    • Atsumi, Y., Tagami, T., Kagami, H., and Ono, T. (2008). Restriction of germline proliferation by soft X-ray irradiation of chicken embryos and its application to chimera production. J. Poult. Sci. 45, 292-297. doi:10. 2141/JPSA. 45. 292
    • (2008) J. Poult. Sci. , vol.45 , pp. 292-297
    • Atsumi, Y.1    Tagami, T.2    Kagami, H.3    Ono, T.4
  • 2
    • 65749092477 scopus 로고    scopus 로고
    • Depletion ofprimordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs
    • doi:10. 2141/JPSA. 46. 136
    • Atsumi, Y., Yazawa, S., Usui, F., Nakamura, Y., Yamamoto, Y., Tagami, T., Hiramatsu, K., Kagami, H., and Ono, T. (2009). Depletion ofprimordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs. J. Poult. Sci. 46, 136-143. doi:10. 2141/JPSA. 46. 136
    • (2009) J. Poult. Sci. , vol.46 , pp. 136-143
    • Atsumi, Y.1    Yazawa, S.2    Usui, F.3    Nakamura, Y.4    Yamamoto, Y.5    Tagami, T.6    Hiramatsu, K.7    Kagami, H.8    Ono, T.9
  • 3
    • 0032966685 scopus 로고    scopus 로고
    • A simple and universal method for molecular sexing ofnon-ratite birds
    • doi:10. 2307/3677252
    • Fridolfsson, A., and Ellegren, H. (1999). A simple and universal method for molecular sexing ofnon-ratite birds. J. Avian Biol. 30, 116-121. doi:10. 2307/3677252
    • (1999) J. Avian Biol. , vol.30 , pp. 116-121
    • Fridolfsson, A.1    Ellegren, H.2
  • 4
    • 0017067265 scopus 로고
    • The origin, migration and morphology of the primordial germ cells in the chick embryo
    • doi:10. 1002/AR. 1091850203
    • Fujimoto, T., Ukeshima, A., and Kiyofuji, R. (1976). The origin, migration and morphology of the primordial germ cells in the chick embryo. Anat Rec. 185, 139-153. doi:10. 1002/AR. 1091850203
    • (1976) Anat Rec. , vol.185 , pp. 139-153
    • Fujimoto, T.1    Ukeshima, A.2    Kiyofuji, R.3
  • 5
    • 0038179385 scopus 로고    scopus 로고
    • Genetics. Poultry genetic resources-operation rescue needed
    • doi:10. 1126/SCIENCE. 1085407
    • Fulton, J. E., and Delany, M. E. (2003). Genetics. Poultry genetic resources-operation rescue needed. Science 300, 1667-1668. doi:10. 1126/SCIENCE. 1085407
    • (2003) Science , vol.300 , pp. 1667-1668
    • Fulton, J.E.1    Delany, M.E.2
  • 6
    • 84924926133 scopus 로고
    • A series ofnormal stages in the development of the chick embryo
    • doi:10. 1002/JMOR. 1050880104
    • Hamburger, V., and Hamilton, H. L. (1951). A series ofnormal stages in the development of the chick embryo. J. Morphol. 88, 49-92. doi:10. 1002/JMOR. 1050880104
    • (1951) J. Morphol. , vol.88 , pp. 49-92
    • Hamburger, V.1    Hamilton, H.L.2
  • 7
    • 0026814630 scopus 로고
    • Cryopreservation ofpoultry sperm: The enigma ofglycerol
    • doi:10. 1016/0011-2240(92)90004-L
    • Hammerstedt, R. H., and Graham, J. K. (1992). Cryopreservation ofpoultry sperm: the enigma ofglycerol. Cryobiology 29, 26-38. doi:10. 1016/0011-2240(92)90004-L
    • (1992) Cryobiology , vol.29 , pp. 26-38
    • Hammerstedt, R.H.1    Graham, J.K.2
  • 8
    • 0030832808 scopus 로고    scopus 로고
    • The developmental origin ofprimordial germ cells and the transmission of the donor-derived gametes in mixed-sex germline chimeras to the offspring in the chicken
    • doi:10. 1002/(SICI)1098-2795(199712) 48:4501:AID-MRD113. 0. CO;2-W
    • Kagami, H., Tagami, T., Matsubara, Y., Harumi, T., Hanada, H., Maruyama, K., Sakurai, M., Kuwana, T., and Naito, M. (1997). The developmental origin ofprimordial germ cells and the transmission of the donor-derived gametes in mixed-sex germline chimeras to the offspring in the chicken. Mol. Reprod. Dev. 48, 501-510. doi:10. 1002/(SICI)1098-2795(199712) 48:4501:AID-MRD113. 0. CO;2-W
    • (1997) Mol. Reprod. Dev. , vol.48 , pp. 501-510
    • Kagami, H.1    Tagami, T.2    Matsubara, Y.3    Harumi, T.4    Hanada, H.5    Maruyama, K.6    Sakurai, M.7    Kuwana, T.8    Naito, M.9
  • 10
    • 33748664631 scopus 로고    scopus 로고
    • Conservation of a threatened indigenous fowl (Kureko dori) using the germline chimeras transplanted from primor-dial germ cells
    • doi:10. 2141/JPSA. 43. 60
    • Kuwana, T., Kawashima, T., Naito, M., Yamashita, H., Matsuzaki, M., and Takano, T. (2006). Conservation of a threatened indigenous fowl (Kureko dori) using the germline chimeras transplanted from primor-dial germ cells. J. Poult. Sci. 43, 60-66. doi:10. 2141/JPSA. 43. 60
    • (2006) J. Poult. Sci. , vol.43 , pp. 60-66
    • Kuwana, T.1    Kawashima, T.2    Naito, M.3    Yamashita, H.4    Matsuzaki, M.5    Takano, T.6
  • 11
    • 0017949755 scopus 로고
    • Preservation offowl semen in liquid nitrogen-an improved method
    • doi:10. 1080/00071667808416462
    • Lake, P. E., and Stewart, J. M. (1978). Preservation offowl semen in liquid nitrogen-an improved method. Br. Poult. Sci. 19, 187-194. doi:10. 1080/00071667808416462
    • (1978) Br. Poult. Sci. , vol.19 , pp. 187-194
    • Lake, P.E.1    Stewart, J.M.2
  • 12
    • 0034904182 scopus 로고    scopus 로고
    • Restriction of proliferation ofprimordial germ cells by the irradiation ofJapanese quail embryos with soft X-rays
    • doi:10. 1016/S1095-6433(01)00375-0
    • Li, H. C., Kagami, H., Matsui, K., and Ono, T. (2001). Restriction of proliferation ofprimordial germ cells by the irradiation ofJapanese quail embryos with soft X-rays.Comp. Biochem. Physiol. A Mol. Integr. Physiol. 130, 133-140. doi:10. 1016/S1095-6433(01)00375-0
    • (2001) Comp. Biochem. Physiol. A Mol. Integr. Physiol. , vol.130 , pp. 133-140
    • Li, H.C.1    Kagami, H.2    Matsui, K.3    Ono, T.4
  • 13
    • 0000125922 scopus 로고
    • The migration ofprimordial germ cells in the chicken embryo
    • Meyer, D. B. (1964). The migration ofprimordial germ cells in the chicken embryo. Dev. Biol. 10, 154-190.
    • (1964) Dev Biol. , vol.10 , pp. 154-190
    • Meyer, D.B.1
  • 14
    • 11144334672 scopus 로고    scopus 로고
    • Genome news highlights loss of chicken strains
    • doi:10. 1038/432799A
    • Miller, M. M. (2004). Genome news highlights loss of chicken strains. Nature 432, 799. doi:10. 1038/432799A
    • (2004) Nature , vol.432 , pp. 799
    • Miller, M.M.1
  • 15
    • 18944382538 scopus 로고    scopus 로고
    • Isolation of chicken primordial germ cells using fluorescence-activated cell sorting
    • Mozdziak, P. E., Angerman-Stewart, J., Rushton, B., Pardue, S. L., and Petitte, J. N. (2005). Isolation of chicken primordial germ cells using fluorescence-activated cell sorting. Poult. Sci. 84, 594-600.
    • (2005) Poult. Sci. , vol.84 , pp. 594-600
    • Mozdziak, P.E.1    Angerman-Stewart, J.2    Rushton, B.3    Pardue, S.L.4    Petitte, J.N.5
  • 16
    • 0024637756 scopus 로고
    • Duration of egg formation in hens selected for increased rate of lay under 23H and 24H light-dark
    • doi:10. 1080/00071668908417124
    • Naito, M., Nirasawa, K., Oishi, T., and Komiyama, T. (1989). Duration of egg formation in hens selected for increased rate of lay under 23H and 24H light-dark. Br. Poult. Sci. 30, 49-60. doi:10. 1080/00071668908417124
    • (1989) Br. Poult. Sci. , vol.30 , pp. 49-60
    • Naito, M.1    Nirasawa, K.2    Oishi, T.3    Komiyama, T.4
  • 17
    • 0025025669 scopus 로고
    • Development in culture of the chick embryo from fertilized ovum to hatching
    • doi:10. 1002/JEZ. 1402540311
    • Naito, M., Nirasawa, K., and Oishi, T. (1990). Development in culture of the chick embryo from fertilized ovum to hatching. J. Exp. Zool. 254, 322-326. doi:10. 1002/JEZ. 1402540311
    • (1990) J. Exp. Zool. , vol.254 , pp. 322-326
    • Naito, M.1    Nirasawa, K.2    Oishi, T.3
  • 18
    • 0028590002 scopus 로고
    • Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring
    • doi:10. 1530/JRF. 0. 1020321
    • Naito, M., Tajima, A., Tagami, T., Yasuda, Y., and Kuwana, T. (1994a). Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring. J. Reprod. Fertil. 102, 321-325. doi:10. 1530/JRF. 0. 1020321
    • (1994) J. Reprod. Fertil. , vol.102 , pp. 321-325
    • Naito, M.1    Tajima, A.2    Tagami, T.3    Yasuda, Y.4    Kuwana, T.5
  • 19
    • 0027940418 scopus 로고
    • Production of germline chimeric chickens, with high transmission rate of donor-derived gametes, produced by transfer of primordial germ cells
    • doi:10. 1002/MRD. 1080390206
    • Naito, M., Tajima, A., Yasuda, Y., and Kuwana, T. (1994b). Production of germline chimeric chickens, with high transmission rate of donor-derived gametes, produced by transfer of primordial germ cells. Mol. Reprod. Dev. 39, 153-161. doi:10. 1002/MRD. 1080390206
    • (1994) Mol. Reprod. Dev. , vol.39 , pp. 153-161
    • Naito, M.1    Tajima, A.2    Yasuda, Y.3    Kuwana, T.4
  • 20
    • 0032751073 scopus 로고    scopus 로고
    • Differentiation of donor primordial germ cells into functional gametes in the gonads of mixed-sex germline chimeric chickens produced by transfer of primordial germ cells isolated from embryonic blood
    • doi:10. 1530/JRF. 0. 1170291
    • Naito, M., Matsubara, Y., Harumi, T., Tagami, T., Kagami, H., Sakurai, M., and Kuwana, T. (1999). Differentiation of donor primordial germ cells into functional gametes in the gonads of mixed-sex germline chimeric chickens produced by transfer of primordial germ cells isolated from embryonic blood. J. Reprod. Fertil. 117, 291-298. doi:10. 1530/JRF. 0. 1170291
    • (1999) J. Reprod. Fertil. , vol.117 , pp. 291-298
    • Naito, M.1    Matsubara, Y.2    Harumi, T.3    Tagami, T.4    Kagami, H.5    Sakurai, M.6    Kuwana, T.7
  • 21
    • 34247528049 scopus 로고    scopus 로고
    • Effects of soft X-ray irradiation to Stage X blastoderm on restriction of proliferation ofprimordial germ cells in early chicken embryos
    • doi:10. 2141/JPSA. 43. 394
    • Nakamichi, H., Sano, A., Harumi, H., Matsubara, Y., Tajima, A., Kosugiyama, M., and Naito, M. (2006). Effects of soft X-ray irradiation to Stage X blastoderm on restriction of proliferation ofprimordial germ cells in early chicken embryos. J. Poult. Sci. 43, 394-400. doi:10. 2141/JPSA. 43. 394
    • (2006) J. Poult. Sci. , vol.43 , pp. 394-400
    • Nakamichi, H.1    Sano, A.2    Harumi, H.3    Matsubara, Y.4    Tajima, A.5    Kosugiyama, M.6    Naito, M.7
  • 23
    • 54349102823 scopus 로고    scopus 로고
    • Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chicken
    • doi:10. 1071/RD08138
    • Nakamura, Y., Yamamoto, Y., Usui, F., Atsumi, Y., Ito, Y., Ono, T., Takeda, K., Nirasawa, K., Kagami, H., and Tagami, T. (2008). Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chicken. Reprod. Fertil. Dev. 20, 900-907. doi:10. 1071/RD08138
    • (2008) Reprod. Fertil. Dev. , vol.20 , pp. 900-907
    • Nakamura, Y.1    Yamamoto, Y.2    Usui, F.3    Atsumi, Y.4    Ito, Y.5    Ono, T.6    Takeda, K.7    Nirasawa, K.8    Kagami, H.9    Tagami, T.10
  • 24
    • 65749104807 scopus 로고    scopus 로고
    • Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos
    • doi:10. 2141/JPSA. 46. 127
    • Nakamura, Y., Usui, F., Atsumi, Y., Otomo, A., Teshima, A., Ono, T., Takeda, K., Nirasawa, K., Kagami, H., and Tagami, T. (2009). Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos. J. Poult. Sci. 46, 127-135. doi:10. 2141/JPSA. 46. 127
    • (2009) J. Poult. Sci. , vol.46 , pp. 127-135
    • Nakamura, Y.1    Usui, F.2    Atsumi, Y.3    Otomo, A.4    Teshima, A.5    Ono, T.6    Takeda, K.7    Nirasawa, K.8    Kagami, H.9    Tagami, T.10
  • 25
    • 77954186388 scopus 로고    scopus 로고
    • Germline replacement by transfer ofprimordial germ cells into partially sterilized embryos in the chicken
    • doi:10. 1095/BIOLREPROD. 110. 083923
    • Nakamura, Y., Usui, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H., and Tagami, T. (2010). Germline replacement by transfer ofprimordial germ cells into partially sterilized embryos in the chicken. Biol. Reprod. 83, 130-137. doi:10. 1095/BIOLREPROD. 110. 083923
    • (2010) Biol. Reprod. , vol.83 , pp. 130-137
    • Nakamura Usui, Y.F.1    Ono, T.2    Takeda, K.3    Nirasawa, K.4    Kagami, H.5    Tagami, T.6
  • 26
    • 0032351782 scopus 로고    scopus 로고
    • Restricted maximum likelihood estimates of genetic parameters and genetic trends of chickens divergently selected for eggshell strength
    • Nirasawa, K., Takahashi, H., Takeda, H., Furukawa, T., Takeda, T., and Nagamine, Y. (1998). Restricted maximum likelihood estimates of genetic parameters and genetic trends of chickens divergently selected for eggshell strength. J. Anim. Breed. Genet. 115, 375-381. (Pubitemid 128656973)
    • (1998) Journal of Animal Breeding and Genetics , vol.115 , Issue.5 , pp. 375-381
    • Nirasawa, K.1    Takahashi, H.2    Takeda, H.3    Furukawa, T.4    Takeda, T.5    Nagamine, Y.6
  • 27
    • 0032929664 scopus 로고    scopus 로고
    • Immunomagnetic purification ofviable primordial germ cells of Japanese quail (Coturnix japonica)
    • doi:10. 1016/S1095-6433(99)00008-2
    • Ono, T., and Machida, Y. (1999). Immunomagnetic purification ofviable primordial germ cells of Japanese quail (Coturnix japonica).Comp. Biochem. Physiol. A Mol. Integr. Physiol. 122, 255-259. doi:10. 1016/S1095-6433(99)00008- 2
    • (1999) Comp. Biochem. Physiol. A Mol. Integr. Physiol. , vol.122 , pp. 255-259
    • Ono, T.1    MacHida, Y.2
  • 28
    • 0037406580 scopus 로고    scopus 로고
    • Improved germline transmission in chicken chimeras produced by transplantation ofgonadal primordial germ cells into recipient embryos
    • doi:10. 1095/BIOLREPROD. 102. 006825
    • Park, T. S., Jeong, D. K., Kim, J. N., Song, K. H., Hong, Y. H., Lim, J. M., and Han, J. Y. (2003). Improved germline transmission in chicken chimeras produced by transplantation ofgonadal primordial germ cells into recipient embryos. Biol. Reprod. 68, 1657-1662. doi:10. 1095/BIOLREPROD. 102. 006825
    • (2003) Biol. Reprod. , vol.68 , pp. 1657-1662
    • Park, T.S.1    Jeong, D.K.2    Kim, J.N.3    Song, K.H.4    Hong, Y.H.5    Lim, J.M.6    Han, J.Y.7
  • 29
    • 0023845199 scopus 로고
    • A complete culture system for the chicken embryo
    • doi:10. 1038/331070A0
    • Perry, M. M. (1988). A complete culture system for the chicken embryo. Nature 331, 70-72. doi:10. 1038/331070A0
    • (1988) Nature , vol.331 , pp. 70-72
    • Perry, M.M.1
  • 30
    • 0025060714 scopus 로고
    • Production of somatic and germline chimeras in the chicken by transfer ofearly blastodermal cells
    • Petitte, J. N., Clark, M. E., Liu, G., Verrinder Gibbins, A. M., and Etches, R. J. (1990). Production of somatic and germline chimeras in the chicken by transfer ofearly blastodermal cells. Development 108, 185-189.
    • (1990) Development , vol.108 , pp. 185-189
    • Petitte, J.N.1    Clark, M.E.2    Liu, G.3    Verrinder Gibbins, A.M.4    Etches, R.J.5
  • 31
    • 33845901316 scopus 로고    scopus 로고
    • Offspring produced from ortho-topic transplantation of chicken ovaries
    • Song, Y., and Silversides, F. G. (2007a). Offspring produced from ortho-topic transplantation of chicken ovaries. Poult. Sci. 86, 107-111.
    • (2007) Poult. Sci. , vol.86 , pp. 107-111
    • Song, Y.1    Silversides, F.G.2
  • 32
    • 33947538933 scopus 로고    scopus 로고
    • Heterotopic transplantation of testes in newly hatched chickens and subsequent production ofoffspring via intramagnal insemination
    • doi:10. 1095/BIOLREPROD. 106. 058032
    • Song, Y., and Silversides, F. G. (2007b). Heterotopic transplantation of testes in newly hatched chickens and subsequent production ofoffspring via intramagnal insemination. Biol. Reprod. 76, 598-603. doi:10. 1095/BIOLREPROD. 106. 058032
    • (2007) Biol. Reprod. , vol.76 , pp. 598-603
    • Song, Y.1    Silversides, F.G.2
  • 33
    • 33751555590 scopus 로고    scopus 로고
    • Differentiation of female primor-dial germ cells in the male testes of chicken (Gallus gallus domesticus)
    • doi:10. 1002/MRD. 20499
    • Tagami, T., Kagami, H., Matsubara, Y., Harumi, T., Naito, M., Takeda, K., Hanada, H., and Nirasawa, K. (2007). Differentiation of female primor-dial germ cells in the male testes of chicken (Gallus gallus domesticus). Mol. Reprod. Dev. 74, 68-75. doi:10. 1002/MRD. 20499
    • (2007) Mol. Reprod. Dev. , vol.74 , pp. 68-75
    • Tagami, T.1    Kagami, H.2    Matsubara, Y.3    Harumi, T.4    Naito, M.5    Takeda, K.6    Hanada, H.7    Nirasawa, K.8
  • 34
    • 38248999645 scopus 로고
    • Production of germline chimera by transfer of primordial germ cells in the domestic chicken(Gallusdomesticus)
    • doi:10. 1016/0093-691X(93)90404-S
    • Tajima, A., Naito, M., Yasuda, Y., and Kuwana, T. (1993). Production of germline chimera by transfer of primordial germ cells in the domestic chicken(Gallusdomesticus). Theriogenology 40, 509-519. doi:10. 1016/0093-691X(93)90404-S
    • (1993) Theriogenology , vol.40 , pp. 509-519
    • Tajima, A.1    Naito, M.2    Yasuda, Y.3    Kuwana, T.4
  • 35
    • 0032519850 scopus 로고    scopus 로고
    • Production of germline chimeras by transfer of cryopreserved gonadal primordial germ cells (gPGCs) in chicken
    • doi:10. 1002/(SICI)1097-010X(19980215)280:3265:AID-JEZ83. 0. CO;2-L
    • Tajima, A., Naito, M., Yasuda, Y., and Kuwana, T. (1998). Production of germline chimeras by transfer of cryopreserved gonadal primordial germ cells (gPGCs) in chicken. J. Exp. Zool. 280, 265-267. doi:10. 1002/(SICI)1097- 010X(19980215)280:3265:AID-JEZ83. 0. CO;2-L
    • (1998) J. Exp. Zool. , vol.280 , pp. 265-267
    • Tajima, A.1    Naito, M.2    Yasuda, Y.3    Kuwana, T.4
  • 36
    • 0027219796 scopus 로고
    • Germline chimeras canproduce both strains of fowl with high efficiency after partial sterilization
    • doi:10. 1530/JRF. 0. 0980637
    • Vick, L., Luke, G., and Simkiss, K. (1993). Germline chimeras canproduce both strains of fowl with high efficiency after partial sterilization. J. Reprod. Fertil. 98, 637-641. doi:10. 1530/JRF. 0. 0980637
    • (1993) J. Reprod. Fertil. , vol.98 , pp. 637-641
    • Vick, L.1    Luke, G.2    Simkiss, K.3
  • 37
    • 34250867360 scopus 로고    scopus 로고
    • A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken
    • doi:10. 1095/BIOLREPROD. 107. 061200
    • Yamamoto, Y., Usui, F., Nakamura, Y., Ito, Y., Tagami, T., Nirasawa, K., Matsubara, Y., Ono, T., and Kagami, H. (2007). A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken. Biol. Reprod. 77, 115-119. doi:10. 1095/BIOLREPROD. 107. 061200
    • (2007) Biol. Reprod. , vol.77 , pp. 115-119
    • Yamamoto, Y.1    Usui, F.2    Nakamura, Y.3    Ito, Y.4    Tagami, T.5    Nirasawa, K.6    Matsubara, Y.7    Ono, T.8    Kagami, H.9
  • 38
    • 0027070770 scopus 로고
    • A method to obtain avian germline chimeras using isolated primordial germ cells
    • doi:10. 1530/JRF. 0. 0960521
    • Yasuda, Y., Tajima, A., Fujimoto, T., and Kuwana, T. (1992). A method to obtain avian germline chimeras using isolated primordial germ cells. J. Reprod. Fertil. 96, 521-528. doi:10. 1530/JRF. 0. 0960521
    • (1992) J. Reprod. Fertil. , vol.96 , pp. 521-528
    • Yasuda, Y.1    Tajima, A.2    Fujimoto, T.3    Kuwana, T.4
  • 39
    • 0038820071 scopus 로고    scopus 로고
    • Purification of avian circulating primordial germ cells by Nycodenz density gradient centrifugation
    • doi:10. 1080/0007166031000085382
    • Zhao, D. F., and Kuwana, T. (2003). Purification of avian circulating primordial germ cells by Nycodenz density gradient centrifugation. Br. Poult. Sci. 44, 30-35. doi:10. 1080/0007166031000085382
    • (2003) Br. Poult. Sci. , vol.44 , pp. 30-35
    • Zhao, D.F.1    Kuwana, T.2


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