EspP, a serine protease of enterohemorrhagic Escherichia coli, impairs complement activation by cleaving complement factors C3/C3b and C5

Infection and Immunity

Volumn 78, Issue 10, 2010, Pages 4294-4301

  Orth, Dorothea ^a   Ehrlenbach, Silvia ^a   Brockmeyer, Jens ^b   Khan, Abdul Basit ^c   Huber, Georg ^a   Karch, Helge ^b   Sarg, Bettina ^a   Lindner, Herbert ^a   Würzner, Reinhard ^a  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^b UNIVERSITY OF MÜNSTER   (Germany)

^c PAKISTAN COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH   (Pakistan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENT COMPONENT C3; COMPLEMENT COMPONENT C3B; COMPLEMENT COMPONENT C5; COMPLEMENT FACTOR H; COMPLEMENT FACTOR I; PROTEIN ESPP; SERINE PROTEINASE; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ARTICLE; BACTERIAL COLONIZATION; COMPLEMENT ACTIVATION; DISEASE SEVERITY; DOWN REGULATION; ENTEROHEMORRHAGIC ESCHERICHIA COLI; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN INTERACTION; SERUM;

COMPLEMENT ACTIVATION; COMPLEMENT C3; COMPLEMENT C3B; COMPLEMENT C5; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HEMOLYTIC-UREMIC SYNDROME; HUMANS; SERINE ENDOPEPTIDASES;

EID: 77957726552     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.00488-10     Document Type: Article

References (28)

1. Brockmeyer, J., M. Bielaszewska, A. Fruth, M. L. Bonn, A. Mellmann, H. U. Humpf, and H. Karch. 2007. Subtypes of the plasmid-encoded serine protease EspP in Shiga toxin-producing Escherichia coli: distribution, secretion, and proteolytic activity. Appl. Environ. Microbiol. 73:6351-6359.
2. Brockmeyer, J., S. Spelten, T. Kuczius, M. Bielaszewska, and H. Karch. 2009. Structure and function relationship of the autotransport and proteolytic activity of EspP from Shiga toxin-producing Escherichia coli. PLoS One 4:e6100.
3. Brunder, W., H. Schimdt, M. Frosch, and H. Karch. 1999. The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements. Microbiology 145:1005-1014.
4. Brunder, W., H. Schmidt, and H. Karch. 1997. EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V. Mol. Microbiol. 24:767-778.
5. Caprioli, J., and G. Remuzzi. 2007. Complement hyperactivation may cause atypical haemolytic uraemic syndrome-gain-of-function mutations in factor B. Nephrol. Dial. Transplant. 22:2452-2454.
6. Cleary, P. P., U. Prahbu, J. B. Dale, D. E. Wexler, and J. Handley. 1992. Streptococcal C5a peptidase is a highly specific endopeptidase. Infect. Immun. 60:5219-5223.
7. Discipio, R. G., P. J. Daffern, M. Kawahara, R. Pike, J. Travis, T. E. Hugli, and J. Potempa. 1996. Cleavage of human complement component C5 by cysteine proteinases from Porphyromonas (Bacteroides) gingivalis. Prior oxidation of C5 augments proteinase digestion of C5. Immunology 87:660-667.
8. Dutta, P. R., R. Cappello, F. Navarro-García, and J. P. Nataro. 2002. Functional comparison of serine protease autotransporters of Enterobacteriaceae. Infect. Immun. 70:7105-7113.
9. Dziva, F., A. Mahajan, P. Cameron, C. Currie, I. J. McKendrick, T. S. Wallis, D. G. Smith, and M. P. Stevens. 2007. EspP, a type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells. FEMS Microbiol. Lett. 271:258-264.
10. Frémeaux-Bacchi, V., E. C. Miller, M. K. Liszewski, L. Strain, J. Blouin, A. L. Brown, N. Moghal, B. S. Kaplan, R. A. Weiss, K. Lhotta, G. Kapur, T. Mattoo, H. Nivet, W. Wong, S. Gie, B. H. de Ligny, M. Fischbach, R. Gupta, R. Hauhart, V. Meunier, C. Loirat, M. A. Dragon-Durey, W. H. Fridman, B. J. Janssen, T. H. Goodship, and J. P. Atkinson. 2008. Mutations in complement C3 predispose to development of atypical hemolytic uremic syndrome. Blood 112:4948-4952.
11. Grys, T. E., L. L. Walters, and R. A. Welch. 2006. Characterization of the StcE protease activity of Escherichia coli O157:H7. J. Bacteriol. 188:4646-4653.
12. Hong, Y. Q., and B. Ghebrehiwet. 1992. Effect of Pseudomonas aeruginosa elastase and alkaline protease on serum complement and isolated components C1q and C3. Clin. Immunol. Immunopathol. 62:133-138.
13. Karch, H., P. I. Tarr, and M. Bielaszewska. 2005. Enterohaemorrhagic Escherichia coli in human medicine. Int. J. Med. Microbiol. 295:405-418.
14. Khan, A. B., A. Naim, D. Orth, K. Grif, M. Mohsin, R. Prager, M. P. Dierich, and R. Würzner. 2009. Serine protease espP subtype alpha, but not beta or gamma, of Shiga toxin-producing Escherichia coli is associated with highly pathogenic serogroups. Int. J. Med. Microbiol. 299:247-254.
15. Kuo, C. F., Y. S. Lin, W. J. Chuang, J. J. Wu, and N. Tsao. 2008. Degradation of complement 3 by streptococcal pyrogenic exotoxin B inhibits complement activation and neutrophil opsonophagocytosis. Infect. Immun. 76:1163-1169.
16. Lathem, W. W., T. E. Grys, S. E. Witowski, A. G. Torres, J. B. Kaper, P. I. Tarr, and R. A. Welch. 2002. StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor. Mol. Microbiol. 45:277-288.
17. Lathem, W. W., T. Bergsbaken, and R. A. Welch. 2004. Potentiation of C1 esterase inhibitor by StcE, a metalloprotease secreted by Escherichia coli O157:H7. J. Exp. Med. 199:1077-1087.
18. Loirat, C., M. Noris, and V. Fremeaux-Bacchi. 2008. Complement and the atypical hemolytic uremic syndrome in children. Pediatr. Nephrol. 23:1957-1972.
19. Oda, T., Y. Kojima, T. Akaike, S. Ijiri, A. Molla, and H. Maeda. 1990. Inactivation of chemotactic activity of C5a by the serratial 56-kilodalton protease. Infect. Immun. 58:1269-1272.
20. Orth, D., A. B. Khan, A. Naim, K. Grif, J. Brockmeyer, H. Karch, M. Joannidis, S. J. Clark, A. J. Day, S. Fidanzi, H. Stoiber, M. P. Dierich, L. B. Zimmerhackl, and R. Würzner. 2009. Shiga toxin activates complement and binds factor H: evidence for an active role of complement in hemolytic uremic syndrome. J. Immunol. 182:6394-6400.
21. Park, S. Y., K. M. Kim, J. H. Lee, S. J. Seo, and I. H. Lee. 2007. Extracellular gelatinase of Enterococcus faecalis destroys a defense system in insect hemolymph and human serum. Infect. Immun. 75:1861-1869.
22. Potempa, M., J. Potempa, T. Kantyka, K. A. Nguyen, K. Wawrzonek, S. P. Manandhar, K. Popadiak, K. Riesbeck, S. Eick, and A. M. Blom. 2009. Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3. PLoS Pathog. 5:e1000316.
23. Schenkein, H. A., H. M. Fletcher, M. Bodnar, and F. L. Macrina. 1995. Increased opsonization of a prtH-defective mutant of Porphyromonas gingivalis W83 is caused by reduced degradation of complement-derived opsonins. J. Immunol. 154:5331-5337.
24. Sim, R. B., and E. Sim. 1981. Autolytic fragmentation of complement components C3 and C4 under denaturing conditions, a property shared with alpha 2-macroglobulin. Biochem. J. 193:129-141.
25. Speth, C., R. Würzner, H. Stoiber, and M. P. Dierich. 2008. Complement, p. 1047-1078. In W. E. Paul (ed.), Fundamental immunology. Lippincott-Raven, Philadelphia, PA.
26. Tarr, P. I., C. A. Gordon, and W. L. Chandler. 2005. Shiga-toxin- producing Escherichia coli and haemolytic uraemic syndrome. Lancet 365:1073-1086.
27. Würzner, R. 1999. Evasion of pathogens by avoiding recognition or eradication by complement, in part via molecular mimicry. Mol. Immunol. 36:249-260.
28. Yamazaki, T., M. Miyamoto, S. Yamada, K. Okuda, and K. Ishihara. 2006. Surface protease of Treponema denticola hydrolyzes C3 and influences function of polymorphonuclear leukocytes. Microbes Infect. 8:1758-1763.