Expression, purification and activity assay of the recombinant protein of Catechol-O-Methyltransferase from chinese white shrimp (Fenneropenaeus chinensis)

American Journal of Biochemistry and Biotechnology

Volumn 6, Issue 3, 2010, Pages 148-154

  Li, Dian Xiang ^a   Du, Xin Jun ^b,c   Zhao, Xiao Fan ^b   Wang, Jin Xing ^b  

^a UNIVERSITY OF JINAN   (China)

^b SHANDONG UNIVERSITY   (China)

^c TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Fenneropenaeus chinensis; HPLC MS; Recombinant Fc COMT; VA

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; CHROMATOGRAPHY; CLONING; ESCHERICHIA COLI; GENE EXPRESSION; GENES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHENOLS; PURIFICATION; SHELLFISH; ZINC OXIDE;

CATECHOL-O-METHYLTRANSFERASE; DIHYDROXYBENZOIC ACIDS; FENNEROPENAEUS CHINENSIS; GENE EXPRESSION PATTERNS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRIES; HPLC-MS; RECOMBINANT FC-COMT; S ADENOSYL L METHIONINES;

RECOMBINANT PROTEINS;

CATECHOL METHYLTRANSFERASE; METHYL GROUP; RECOMBINANT PROTEIN; S ADENOSYLMETHIONINE; VANILLIC ACID;

ARTICLE; CATALYSIS; CELL LYSATE; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME MECHANISM; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROKARYOTE; PROTEIN PURIFICATION; SHRIMP;

DECAPODA (CRUSTACEA); ESCHERICHIA COLI; FENNEROPENAEUS CHINENSIS; PROKARYOTA;

EID: 77957365493     PISSN: 15533468     EISSN: None     Source Type: Journal    
DOI: 10.3844/ajbbsp.2010.148.154     Document Type: Article

References (24)

1. Axelrod, J. and R. Tomchick, 1958. Enzymatic Omethylation of epinephrine and other catechols. J. Biol. Chem., 233: 702-705. PMID: 13575440
2. Bertocci, B., V. Miggiano, M. da Prada, Z. Dembic and H.W. Lahm et al., 1991. Human catechol-Omethyltransferase: Cloning and expression of the membrane-associated form. Proc. Natl. Acad. Sci. USA., 88: 1416-1420. PMID: 1847521
3. Bonifacio, M.J., M. Archer, M.L. Rodrigues, P.M. Matias and D.A. Learmonth et al., 2002. Kinetics and crystal structure of catechol-O-methyltransferase complex with co-substrate and a novel inhibitor with potential therapeutic application. Mol. Pharmacol., 62: 795-805. PMID: 12237326
4. Borchardt, R.T., 1974. A rapid spectrophotometric assay for catechol-O-methyltransferase. Anal. Biochem., 58: 382-389. PMID: 4827387
5. Bradford, M.M., 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254. PMID: 942051
6. Chen, J., B.K. Lipska, N. Halim, Q.D. Ma and M. Matsumoto et al., 2003. Functional analysis of genetic variation in Catechol-O-Methyltransferase (COMT): Effects on mRNA, protein and enzyme activity in postmortem human brain. Am. J. Hum. Genet., 75: 807-821. PMID: 15457404
7. Cheng, T.C., S.T. Chen, C.S. Huang, Y.P. Fu and J.C. Yu et al., 2005. Breast cancer risk associated with genotype polymorphism of the catechol estrogen-metabolizing genes: A multigenic study on cancer susceptibility. Int. J. Cancer, 113: 345-353. PMID: 15455371
8. Guldberg, H.C. and C.A. Marsden, 1975. Catechol-Omethyl transferase: Pharmacological aspects and physiological role. Pharmacol. Rev., 27: 135-206. PMID: 1103160
9. Gulliver, P.A. and K.F. Tipton, 1978. Direct extraction radioassay for catechol-Q-methyl-transferase activity. Biochem. Pharmacol., 27: 773-775. PMID: 656116
10. Karayiorgou, M., J.A. Gogos, B.L. Galke, P.S. Wolyniec and G. Nestadt et al., 1998. Identification of sequence variants and analysis of the role of the catechol-O-methyl-transferase gene in schizophrenia susceptibility. Biol. Psychiatry, 43: 425-431. PMID: 9532347
11. Koh, S., K. Urayama, S. Kawai and Y. Takayama, 1991. Application of a metal capillary column in gas chromatographic determination of catecholo-methyltransferase activity. J. Chromatogr., 549: 434-439. PMID: 1770086
12. Laemmli, U.K., 1970. Cleavage of structural proteins during the assemble of the head of bacteriophage T4. Nature, 227: 680-685. PMID: 5432063
13. Li, D.X., X.J. Du, X.F. Zhao and J.X. Wang, 2006. Cloning and expression analysis of an OMethyltransferase (OMT) gene from Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol., 21: 284-292. PMID: 16530427
14. Li, K., X. Chen, Y. Jia and K. Bi, 2004. Reverse-phase HPLC determination and pharmacokinetic study of vanillic acid in the plasma of rats treated with the traditional Chinese medicinal preparation Di-Gu-Pi decoction. Yakugaku Zasshi, 124: 465-468. PMID: 15235230
15. Mannisto, P.T., I. Ulmanen, K. Lundstrom, J. Taskinen and J. Tenhunen et al., 1992. Characteristics of Catechol O-Methyltransferase (COMT) and properties of selective COMT inhibitors. Prog. Drug. Res., 39: 291-350. PMID: 1475365
16. Matsumoto, M., C.S. Weickert, M. Akil, B.K. Lipska and T.M. Hyde et al., 2003. Catechol Omethyltransferase mRNA expression in human and rat brain: Evidence for a role in cortical neuronal function. Neuroscience, 116: 127-137. PMID: 12535946
17. Okada, Y., K. Zaitsu, K. Ohtsubo, H. Nohta and Y. Ohkura, 1981. Fluorimetric assay for catechol-O-methyltransferase. Chem. Pharm. Bull., 29: 1670-1673. PMID: 7296717
18. Pihlavisto, P. and I. Reenila, 2002. Separation methods for catechol O-methyltransferase activity assay: Physiological and pathophysiological relevance. J. Chromatogr. B. Anal. Technol. Biomed. Life Sci., 781: 359-372. PMID: 12450669
19. Reenila, I. and P. Rauhala, 2009. Simultaneous analysis of catechol-O-methyltransferase activity, Sadenosylhomocysteine and adenosine. Biomed. Chromatogr., 24: 294-300. PMID: 19629963
20. Reenila, I., P. Tuomainen and P.T. Mannisto, 1995. Improved assay of reaction products to quantitate catechol-O-methyltransferase activity by highperformance liquid chromatography with electrochemical detection. J. Chromatogr. B. Biomed. Sci. Appli., 663: 137-142. PMID: 7704200
21. Schrag, A., 2005. Entacapone in the treatment of Parkinson's disease. Lancet Neurol., 4: 366-370. PMID: 15907741
22. Tilgmann, C. and I. Ulmanen, 1996. Purification methods of mammalian catechol-Omethyltransferases. J. Chromatogr. B. Biomed. Appli., 684: 147-161. PMID: 8906471
23. Tuomainen, P., I. Reenila and P.T. Mannisto, 1996. Validation of assay of catechol-Omethyltransferase activity in human erythrocytes. J. Pharm. Biomed. Anal., 14: 515-523. PMID: 8738181
24. Vilbois, F., P. Caspers, M. da Prada, G. Lang and C. Karrern et al., 1994. Mass spectrometric analysis of human soluble catechol Omethyltransferase expressed in Escherichia coli. Eur. J. Biochem., 222: 377-386. PMID: 8020475