Controlling medium osmolality improves the expansion of human articular chondrocytes in serum-free media

Tissue Engineering - Part C: Methods

Volumn 16, Issue 5, 2010, Pages 957-963

  Koo, Jane ^a   Kim, Kang Il ^b   Min, Byoung Hyun ^c,d   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Kyung Hee University College of Medicine   (South Korea)

^c Ajou University   (South Korea)

^d Ajou University School of Medicine   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CARTILAGE; CELL CULTURE; EXPANSION; MONOLAYERS; SODIUM CHLORIDE;

CHONDROCYTE GROWTH; CHONDROGENIC; GLYCOSAMINOGLYCANS; HUMAN ARTICULAR CARTILAGE; HUMAN ARTICULAR CHONDROCYTES; IMMUNOHISTOCHEMICAL ANALYSIS; IN-VIVO; MONOLAYER CULTURE; NACL CONCENTRATION; OSMOLALITY; PROLIFERATION RATE; SERUM-FREE MEDIA; TYPE II;

BODY FLUIDS;

COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN;

AGED; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CELL PROLIFERATION; CULTURE MEDIUM; CYTOLOGY; FEMALE; HUMAN; MALE; METABOLISM; MIDDLE AGED; OSMOLARITY;

AGED; CARTILAGE, ARTICULAR; CELL PROLIFERATION; CHONDROCYTES; COLLAGEN TYPE II; CULTURE MEDIA, SERUM-FREE; FEMALE; GLYCOSAMINOGLYCANS; HUMANS; MALE; MIDDLE AGED; OSMOLAR CONCENTRATION;

EID: 77957343494     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2009.0525     Document Type: Article

References (27)

1. Brittberg, M., Lindahl, A., Nilsson, A., Ohlsson, C., Isaksson, O., and Peterson, L. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331, 889, 1994.
2. Binette, F., McQuaid, D.P., Haudenschild, D.R., Yaeger, P.C., McPherson, J.M., and Tubo, R. Expression of a stable artic-ular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes in vitro. J Orthop Res 16, 207, 1998.
3. Jakob, M., Démarteau, O., Schäfer, D., Hintermann, B., Dick, W., Heberer, M., and Martin, I. Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formation in vitro. J Cell Biochem 81, 368, 2001.
4. Schnabel, M., Marlovits, S., Eckhoff, G., Fichtel, I., Gotzen, L., Vecsei, V., and Schlegel, J. Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthritis Cartilage 10, 62, 2002.
5. Diaz-Romero, J., Gaillard, J.P., Grogan, S.P., Nesic, D., Trub, T., and Mainil-Varlet, P. Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture. J Cell Physiol 202, 731, 2005.
6. Yang, K.G., Saris, D.B., Geuze, R.E., Helm, Y.J., Rijen, M.H., Verbout, A.J., Dhert, W.J., and Creemers, L.B. Impact of expansion and redifferentiation conditions on chondrogenic capacity of cultured chondrocytes. Tissue Eng 12, 2435, 2006.
7. Carossino, A.M., Recenti, R., Carossino, R., Piscitelli, E., Gozzini, A., Martineti, V., Mavilia, C., Franchi, A., Danielli, D., Aglietti, P., Ciardullo, A., Galli, G., Tognarini, I., Moggi Pignone, A., Cagnoni, M., and Brandi, M.L. Methodological models for in vitro amplification and maintenance of human articular chondrocytes from elderly patients. Biogerontology 8, 483, 2007.
8. Barlic, A., Drobnic, M., Malicev, E., and Kregar-Velikonja, N. Quantitative analysis of gene expression of human articular chondrocytes assigned for autologous implantation. J Or-thop Res 26, 847, 2008.
9. Lin, Z., Fitzgerald, J.B., Xu, J., Willers, C., Wood, D., Grod-zinsky, A.J., and Zheng, M.H. Gene expression profiles of human chondrocytes during passaged monolayer cultivation. J Orthop Res 26, 1230, 2008.
10. Yaeger, P.C., Masi, T.L., de Ortiz, J.L., Binette, F., Tubo, R., and McPherson, J.M. Synergistic action of transforming growth factor-beta and insulin-like growth factor-I induces expression of type II collagen and aggrecan genes in adult human articular chondrocytes. Exp Cell Res 237, 318, 1997.
11. Schulze-Tanzil, G., de Souza, P., Villegas Castrejon, H., John, T., Merker, H.J., Scheid, A., and Shakibaei, M. Re-differentiation of dedifferentiated human chondrocytes in high-density cultures. Cell Tissue Res 308, 371, 2002.
12. Barbero, A., Ploegert, S., Heberer, M., and Martin, I. Plasticity of clonal populations of dedifferentiated adult human articular chondrocytes. Arthritis Rheum 48, 1315, 2003.
13. Malpeli, M., Randazzo, N., Cancedda, R., and Dozin, B. Serum-free growth medium sustains commitment of human articular chondrocyte through maintenance of Sox9 expression. Tissue Eng 10, 145, 2004.
14. Choi, Y.S., Lim, S.M., Lee, C.W., and Kim, D. Development of serum-free media for primary culture of human articular chondrocytes. J Microbiol Biotechnol 15, 1299, 2005.
15. Giannoni, P., Pagano, A., Maggi, E., Arbico, R., Randazzo, N., Grandizio, M., Cancedda, R., and Dozin, B. Autologous chondrocyte implantation (ACI) for aged patients: development of the proper cell expansion conditions for possible therapeutic applications. Osteoarthritis Cartilage 13, 589, 2005.
16. Freshney, R.I. The culture environment: substrate, gas phase, medium, and temperature. In: Culture of Animal Cells. New York, NY: Wiley-Liss, 1994, pp. 83-84.
17. Urban, J.P.G., Hall, A.C., and Gehl, K.A. Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes. J Cell Physiol 154, 262, 1993.
18. Martin, I., Vunjak-Novakovic, G., Yang, J., Langer, R., and Freed, L.E. Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue. Exp Cell Res 253, 681, 1999.
19. Hollander, A.P., Heathfield, T.F., Webber, C., Iwata, Y., Bourne, R., Rorabeck, C., and Poole, A.R. Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay. J Clin Invest 93, 1722, 1994.
20. Farndale, R.W., Buttle, D.J., and Barrett, A.J. Improved quantitation and discrimination of sulphated glycosamino-glycans by use of dimethylmethylene blue. Biochim Biophys Acta 883, 173, 1986.
21. Brittberg, M., Peterson, L., Sjögren-Jansson, E., Tallheden, T., and Lindahl, A. Articular cartilage engineering with autol-ogous chondrocyte transplantation: a review of recent developments. J Bone Joint Surg Am 85 (Suppl A), 109, 2003.
22. Racz, B., Reglodi, D., Fodor, B., Gasz, B., Lubics, A., Gallyas, F., Jr., Roth, E., and Borsiczky, B. Hyperosmotic stress-induced apoptotic signaling pathways in chondrocytes. Bone 40, 1536, 2007.
23. Palmer, G.D., Chao, P.H., Raia, F., Mauck, R.L., Valhmu, W.B., and Hung, C.T. Time-dependent aggrecan gene expression of articular chondrocytes in response to hyper-osmotic loading. Osteoarthritis Cartilage 9, 761, 2001.
24. Shakibaei, M., Seifarth, C., John, T., Rahmanzadeh, M., and Mobasheri, A. Igf-I extends the chondrogenic potential of human articular chondrocytes in vitro: molecular association between Sox9 and Erk1=2. Biochem Pharmacol 72, 1382, 2006.
25. Francioli, S.E., Martin, I., Sie, C.P., Hagg, R., Tommasini, R., Candrian, C., Heberer, M., and Barbero, A. Growth factors for clinical-scale expansion of human articular chondrocytes: relevance for automated bioreactor systems. Tissue Eng 13, 1227, 2007.
26. Hung, C.T., LeRoux, M.A., Palmer, G.D., Chao, P.H., Lo, S., and Valhmu, W.B. Disparate aggrecan gene expression in chondrocytes subjected to hypotonic and hypertonic loading in 2D and 3D culture. Biorheology 40, 61, 2003.
27. Hopewell, B., and Urban, J.P. Adaptation of articular chon-drocytes to changes in osmolality. Biorheology 40, 73, 2003.