Time-dependent aggrecan gene expression of articular chondrocytes in response to hyperosmotic loading

Osteoarthritis and Cartilage

Volumn 9, Issue 8, 2001, Pages 761-770

  Palmer, G D ^a   Chao, P H G ^a   Raia, F ^a   Mauck, R L ^a   Valhmu, W B ^b   Hung, C T ^a  

^a Columbia University ^*  (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

Aggrecan; Cartilage; Chondrocyte; Osmolality

Indexed keywords

AGGRECAN; MESSENGER RNA;

ANIMAL CELL; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CELL CULTURE; CELL ISOLATION; CELL SIZE; CELL SWELLING; CONTROLLED STUDY; CULTURE MEDIUM; EXON; EXTRACELLULAR FLUID; FLUORESCENCE MICROSCOPY; GENE DELETION; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; HYPEROSMOLALITY; LOADING TEST; NONHUMAN; OSMOREGULATION; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERUM; STIMULUS RESPONSE;

EID: 0035159519     PISSN: 10634584     EISSN: None     Source Type: Journal    
DOI: 10.1053/joca.2001.0473     Document Type: Article

References (55)

1. Structure-function relationships of articular cartilage and the effects of joint instability and trauma on cartilage function
2. The structure, function and biologic repair of articular cartilage
3. Proteoglycans: Many forms and many functions
4. The specific interaction of hyaluronic acid with cartilage proteoglycans
5. The effects of hydrostatic and osmotic pressures on chodrocyte metabolism
6. The extracellular matrix, interstitial fluid and ions as a mechanical signal transducer in articular cartilage
7. Physical Regulation of Cartilage Metabolism
8. Biochemical changes in articular cartilage after joint immobilization by casting or external fixation
9. Discoordinate gene expression of aggrecan and type II collagen in experimental osteoarthritis
10. Joint motion in the absence of normal loading does not maintain normal articular cartilage
11. In vivo and in vitro stimulation of chondrocyte biosynthetic activity in early experimental osteoarthritis
12. Mechanical behavior and biochemical composition of canine knee cartilage following periods of joint disuse and disuse with remobilization
13. The effects of matrix compression on proteoglycan metabolism in articular cartilage explants
14. Biosynthetic response of cartilage explants to dynamic compression
15. Effect of static and cyclic compressive loading on articular cartilage plugs in vitro
16. Local stimulation of proteoglycan synthesis in articular cartilage explants by dynamic compression in vitro
17. Expression of reduced amounts of structurally altered aggrecan in articular cartilage chondrocytes exposed to high hydrostatic presssure
18. In vitro stimulation of articular chondrocyte mRNA and extracellular matrix synthesis by hydrostatic pressure
19. Mitogenactivated protein kinase signaling in bovine articular chondrocytes in response to fluid flow does not require calcium mobilization
20. Effects of fluid-induced shear on articular chondrocyte morphology and metabolism in vitro
21. Mechanical and physiocochemical determinants of the chondrocyte biosynthetic response
22. Adaptive cellular response to osmotic stress in pig articular chondrocytes
23. Effects of mechanical and osmotic pressure on the rate of glycosaminoglycan synthesis in adult femoral head cartilage: An in vitro study
24. Regulation of proteoglycan synthesis rate in cartilage in vitro: Influence of extracellular ionic composition
25. Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes
26. Effects of extracellular osmolality on porcine articular chondrocyte volume
27. Volume regulatory properties of porcine articular chondrocytes measured in situ using confocal microscopy
28. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
29. + transport across the bovine articular chondrocyte membrane and their sensitivity to cell volume
30. Modulation of Na(+)×H(+) exchange by osmotic shock in isolated bovine articular chondrocytes
31. Mechanotransduction via integrins and interleukin-4 results in altered aggrecan and matrix metalloproteinase 3 gene expression in normal, but not osteoarthritic, human articular chondrocytes
32. Down-regulation of chondrocyte aggrecan and type-II collagen gene expression correlates with increases in static compression magnitude and duration
33. Load-controlled compression of articular cartilage induces a transient stimulation of aggrecan gene expression
34. A single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction
35. Quantitative polymerase chain reaction assay for aggrecan and link protein gene expression in cartilage
36. Osmotic loading regulates chondrocyte cell size and aggrecan gene expression
37. Calcium dependent and independent volume regulation in articular chondrocytes
38. Comparison of transient chondrocyte swelling and shrinking behavior
39. Hypotonic swelling increases internal calcium concentration and activates membrane ion channel currents in bovine articular chondrocytes
40. Quantification of transient swelling behavior in cultured chondrocytes to osmotic loading
41. The effects of osmotic pressure on the viscoelastic and physical properties of articular chondrocytes
42. Platelet-derived growth factor B chain promoter contains a cis-acting fluid shearstress-responsive element
43. The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces Cerevisiae CTT1 gene
44. The effect of continuous mechanical pressure upon the turnover of articular cartilage proteoglycans in vitro
45. Proteoglycan depletion alone is not sufficient to stimulate proteoglycan synthesis in cultured bovine cartilage explants
46. Modulation of extracellular matrix gene expression in bovine high density chondrocyte cultures by ascorbic acid and enzymatic resuspension
47. Dihydrocytochalasin B enhances transforming growth factor-beta-induced reexpression of the differentiated chondrocyte phenotype without stimulation of collagen synthesis
48. Influence of cytochalasin D-induced changes in cell shape on proteoglycan synthesis by cultured articular chondrocytes
49. Cytoskeleton and differentiation: Effects of cytochalasin B and colchicine on expression of the differentiated phenotype of rabbit costal chondrocytes in culture
50. Mechano-chemical coupling in the flow-induced activation of intracellular calcium signaling in primary cultured bone cells
51. Chondrocytes in culture produce a mechanically functional tissue
52. Convective mass transfer effects on the intracellular calcium response of endothelial cells
53. Modulation of aggrecan and link-protein synthesis in articular cartilage
54. Control of proteoglycan biosynthesis. Further studies on the effect of serum on cultured bovine articular cartilage
55. Aggrecan gene expression of chondrocyte seeded 3D hydrogel cultures in response to hypertonic loading