Functional biostructure of colonic microbiota (central fermenting area, germinal stock area and separating mucus layer) in healthy subjects and patients with diarrhea treated with Saccharomyces boulardii;Biostructure fonctionnelle du microbiote colique (zone de fermentation centrale, zone de réserve germinale et couche de mucus séparatrice) chez les sujets sains et chez les patients atteints de diarrhée traités par Saccharomyces boulardii.

Gastroenterologie Clinique et Biologique

Volumn 34, Issue SUPPL. 1, 2010, Pages

  Swidsinski, A ^a   Loening Baucke, V ^a   Kirsch, S ^b   Doerffel, Y ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b Asklepios Clinic Teupitz Neurology   (Germany)

Author keywords

Alcian PAS; Cy3, Cy5, FITC; DAPI different fluorescent dyes corresponding to green, orange, dark red and blue colours; DSS; FISH fluorescence in situ hybridization

Indexed keywords

PERENTEROL; PROBIOTIC AGENT;

ABSCESS; ADULT; ARTICLE; BACTERIAL METABOLISM; BACTERIUM; BACTEROIDES; BIOREACTOR; CHRONIC DIARRHEA; CLINICAL ARTICLE; CLINICAL ASSESSMENT; CLOSTRIDIUM PERFRINGENS; COLON FLORA; COLONOSCOPY; DIARRHEA; DISEASE DURATION; ECHOGRAPHY; FAECALIBACTERIUM PRAUSNITZII; FECES ANALYSIS; FECES MICROFLORA; FEMALE; FERMENTATION; FLUORESCENCE IN SITU HYBRIDIZATION; FOLLOW UP; GAS GANGRENE; GASTROSCOPY; GERMINAL VESICLE; HUMAN; LABORATORY TEST; LARGE INTESTINE; MALE; MICROORGANISM; MICROSCOPY; MUCUS; PROSTHETIC VALVE ENDOCARDITIS; ROSEBURIA; SACCHAROMYCES BOULARDII; SEPSIS; STRUCTURE ANALYSIS; TREATMENT RESPONSE; VISCOSITY; CASE CONTROL STUDY; CHEMISTRY; CHRONIC DISEASE; COLON; DRUG EFFECT; FECES; METABOLISM; METAGENOME; MICROBIOLOGY; MIDDLE AGED; SACCHAROMYCES; TIME; TREATMENT OUTCOME;

ADULT; CASE-CONTROL STUDIES; CHRONIC DISEASE; COLON; DIARRHEA; FECES; FEMALE; FERMENTATION; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; METAGENOME; MIDDLE AGED; MUCUS; PROBIOTICS; SACCHAROMYCES; TIME FACTORS; TREATMENT OUTCOME;

EID: 77957340111     PISSN: 03998320     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0399-8320(10)70025-7     Document Type: Article

References (15)

1. Tamboli C.P., Neut C., Desreumaux P., Colombel J.F. Dysbiosis in inflammatory bowel disease. Gut 2004, 53:1-4.
2. Xavier R.J., Podolsky D.K. Unravelling the pathogenesis of inflammatory bowel disease. Nature 2007, 448:427-434.
3. Dethlefsen L., Huse S., Sogin M.L., Relman D.A. The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol 2008, 6:e280.
4. Hattori M., Taylor T.D. The human intestinal microbiome: a new frontier of human biology. DNA Res 2009, 16:1-12.
5. Swidsinski A., Sydora B.C., Doerffel Y., Loening-Baucke V., Vaneechoutte M., Lupicki M., et al. Viscosity gradient within the mucus layer determines the mucosal barrier function and the spatial organization of the intestinal microbiota. Inflamm Bowel Dis 2007, 13:963-970.
6. Swidsinski A., Loening-Baucke V., Lochs H., Hale L.P. Spatial organization of bacterial flora in normal and inflamed intestine: a fluorescence in situ hybridization study in mice. World J Gastroenterol 2005, 11:1131-1140.
7. Swidsinski A., Loening-Baucke V., Vaneechoutte M., Doerffel Y. Active Crohn's disease and ulcerative colitis can be specifically diagnosed and monitored based on the biostructure of the fecal flora. Inflamm Bowel Dis 2008, 14:147-161.
8. Afzalpurkar R.G., Schiller L.R., Little K.H., Santangelo W.C., Fordtran J.S. The self-limited nature of chronic idiopathic diarrhea. N Engl J Med 1992, 327:1849-1852.
9. Czerucka D., Piche T., Rampal P. Review article: yeast as probiotics -- Saccharomyces boulardii. Aliment Pharmacol Ther 2007, 26:767-778.
10. Habba S.F. Chronic diarrhea: identifying a new syndrome. Am J Gastroenterol 2000, 95:2140-2141.
11. Swidsinski A. Standards for bacterial identification by fluorescence in situ hybridization within eukaryotic tissue using ribosomal rRNA-based probes. Inflamm Bowel Dis 2006, 12:824-826.
12. Swidsinski A., Weber J., Loening-Baucke V., Hale L.P., Lochs H. Spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease. J Clin Microbiol 2005, 43:3380-3389.
13. Suau A., Rochet V., Sghir A., Gramet G., Brewaeys S., Sutren M., et al. Fusobacterium prausnitzii and related species represent a dominant group within the human fecal flora. Syst Appl Microbiol 2001, 24:139-145.
14. Derrien M., Collado M.C., Ben-Amor K., Salminen S., de Vos W.M. The Mucin degrader Akkermansia muciniphila is an abundant resident of the human intestinal tract. Appl Environ Microbiol 2008, 74:1646-1648.
15. Loy A., Maixner F., Wagner M., Horn M. probeBase - an online resource for rRN A-targeted oligonucleotide probes: new features 2007. Nucleic Acids Res 2007, 35:D800-D804.