Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes

Genetics and Molecular Biology

Volumn 33, Issue 3, 2010, Pages 486-490

  Zaros, Lilian Giotto ^a   Coutinho, Luiz Lehmann ^b   Sider, Lúcia Helena ^c   de Medeiros, Henrique Rocha ^a   das Neves, Maria Rosalba Moreira ^d   Benvenuti, Camila Loures ^d   Navarro, Andrine Maria do Carmo ^d   Vieira, Luiz da Silva ^c  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c EMBRAPA CERRADOS   (Brazil)

^d UNIVERSIDADE ESTADUAL VALE DO ACARAÚ   (Brazil)

Author keywords

Control genes; Gastrointestinal nematodes; Ovine; Real time RT PCR

Indexed keywords

COMPLEMENTARY DNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; RIBOSOME PROTEIN; RIBOSOME PROTEIN LO; SUCCINATE DEHYDROGENASE; SUCCINATE DEHYDROGENASE COMPLEX SUBUNIT A; UNCLASSIFIED DRUG;

ARTICLE; BRAZIL; CULTURE MEDIUM; FECES ANALYSIS; GASTROINTESTINAL INFECTION; GENE EXPRESSION; GENE SEQUENCE; GENETIC STABILITY; LYMPH NODE; NEMATODE; NEMATODIASIS; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REFERENCE VALUE; RUMINANT STOMACH; SHEEP; SMALL INTESTINE;

ANIMALIA; NEMATODA; OVIS; OVIS ARIES;

EID: 77957304052     PISSN: 14154757     EISSN: 16784685     Source Type: Journal    
DOI: 10.1590/S1415-47572010000300018     Document Type: Article

References (20)

1. Andersen CL, Jensen JL and Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245-5250.
2. Bricarello AP, Zaros LG, Coutinho LL, Rocha RA, Silva MB, Kooyman FNJ, De Vries E, Yatsuda AP and Amarante AFT (2008) Immunological responses and cytokine gene expression analysis to Cooperia punctata infections in resistant and susceptible Nelore cattle. Vet Parasitol 155:95-103.
3. Bustin SA (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR); trends and problems. J Mol Endocrinol 29:23-39.
4. Bustin SA, Benes A, Nolan T and Pfaffl MW(2005) Quantitative real-time RT-PCR - A perspective. J Mol Endocrinol 34:597-601.
5. Cao H, Kabaroff LC, You Q, Rodrigues A, Boermans H and Karrow NA (2006) Characterization of ovine hepatic gene expression profiles in response to E. coli lipopolysaccharide using a bovine cDNA microarray. BMC Vet Res 2:34.
6. Chomczynski P and Sacchi N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
7. Garcia-Crespo D, Juste RA and Hurtado A (2005) Selection of ovine housekeeping genes for normalization by real-time RT-PCR; analysis of PrP gene expression and genetic susceptibility to scrapie. BMC Vet Res 1:746-6148-1-3.
8. Giulietti A, Overbergh L, Valckx D, Dacallonne B, Bouillon R and Mathieu C (2001) An overview of real time quantitative PCR: Applications to quantify cytokine gene expression. Methods 25:386-401.
9. Ingham A, Reverter A, Windon R, Hunt P and Menzies M (2008) Gastrointestinal nematode challenge induces some conserved gene expression changes in the gut mucosa of genetically resistant sheep. Int J Parasitol 38:431-442.
10. Jasmer DP, Lahmers KK and Brown WC (2007) Haemonchos contortus intestine: A proeminent source of mucosal antigens. Parasitol Imunol 29:139-151.
11. Ke LD, Chen Z and Yung WKA (2000) A reliability test of standard- based quantitative PCR: Exogenous vs. endogenous standards. Mol Cell Probes 14:127-135.
12. Mulubamba K, Smeed J, Gossner A, Watkins C, Dalziel R and Hopkins J (2008) Differential expression of pattern recognition receptors in the three pathological forms of sheep paratuberculosis. Microbes Infect 10:598-604.
13. Pfaffl MW(2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:2002-2007.
14. Regitano LCA, Ibelli AMG, Gasparin G, Miyata M, Azevedo ALS, Coutinho LL, Teodoro RL, Machado MA, Silva MVGB, Nakata LC et al. (2008) On the search for markers of tick resistance in bovines. Dev Biol 132:225-230.
15. SAS (1991) Statistical Analysis System, SAS Procedures Guide, v. 6.11. Cary Institute Inc, New York.
16. Smeed JA, Watkins CA, Rhind SM and Hopkins J (2007) Differential cytokine gene expression profiles in the three pathological forms of sheep paratuberculosis. BMC Vet Parasitol Res 3:18.
17. Suzuki T, Higgens PJ and Crawford DR (2000) Control selection for RNA quantification. Biotechniques 29:332-337.
18. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A and Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:e34.
19. Wilkening S and Bader A (2004) Quantitative real-time polymerase chain reaction: Methodical analysis and mathematical model. J Biomol Tech 15:107-111.
20. Zaros LG, Bricarello PA, Amarante AFT and Coutinho LL (2007) Quantification of bovine cytokine expression using realtime RT-PCR methodology. Genet Mol Biol 30:575-579.