Shine-Dalgarno sequence enhances the efficiency of lacZ repression by artificial anti-lac antisense RNAs in Escherichia coli

Journal of Bioscience and Bioengineering

Volumn 110, Issue 5, 2010, Pages 523-528

  Stefan, Alessandra ^a,b   Schwarz, Flavio ^a   Bressanin, Daniela ^a   Hochkoeppler Alejandro, A ^a,b  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF FLORENCE   (Italy)

Author keywords

Antisense RNAs; Escherichia coli; Lac operon; Shine Dalgarno sequence; Silencing

Indexed keywords

ANTISENSE; ANTISENSE RNA; CULTURE MEDIUM; E. COLI; EXPRESSION VECTORS; GALACTOSIDASES; IN-CELL; IN-VIVO; LAC OPERON; REVERSE ORIENTATION; SDS-PAGE; SHINE-DALGARNO SEQUENCE; SILENCING; SIMILAR ANALYSIS; SITE-SPECIFIC; WESTERN BLOTTING;

CELL CULTURE; TRANSCRIPTION;

ESCHERICHIA COLI;

ARABINOSE; BETA GALACTOSIDASE; COMPLEMENTARY RNA;

ARTICLE; BACTERIAL GENE; CELL TRANSFORMATION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CONSTRUCT; GENE EXPRESSION; GENE REPRESSION; GENE SEQUENCE; GENE SILENCING; GENETIC TRANSCRIPTION; IN VIVO STUDY; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; RNA STABILITY; SHINE DALGARNO REGION; SILENCER GENE; WESTERN BLOTTING;

BASE SEQUENCE; BETA-GALACTOSIDASE; DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI; GENE SILENCING; GENETIC ENGINEERING; GENETIC VECTORS; LAC OPERON; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEIC ACID CONFORMATION; RNA, ANTISENSE; TRANSFORMATION, GENETIC;

ESCHERICHIA COLI; PROKARYOTA;

EID: 77957293076     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.05.012     Document Type: Article

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