Antimicrobial resistance and virulence gene profiles in multi-drug resistant enterotoxigenic Escherichia coli isolated from pigs with post-weaning diarrhoea

Veterinary Microbiology

Volumn 145, Issue 3-4, 2010, Pages 299-307

  Smith, M G ^a   Jordan, D ^b   Chapman, T A ^c   Chin, J J C ^c   Barton, M D ^d   Do, T N ^e   Fahy, V A ^f   Fairbrother, J M ^g   Trott, D J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b Industry and Investment New South Wales   (Australia)

^c NSW DEPARTMENT OF PRIMARY INDUSTRIES   (Australia)

^d UNIVERSITY OF SOUTH AUSTRALIA   (Australia)

^e NATIONAL INSTITUTE OF VETERINARY RESEARCH   (Viet Nam)

^f DEPARTMENT OF PRIMARY INDUSTRIES   (Australia)

^g UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Antimicrobial; Diarrhoea; Multi drug resistance; Post weaning; Virulence

Indexed keywords

ADHESIN; ALPHA HEMOLYSIN; AMPICILLIN; APRAMYCIN; BETA LACTAMASE AMPC; BMAE PROTEIN; CEFTIOFUR; CHLORAMPHENICOL; COTRIMOXAZOLE; ENROFLOXACIN; FEDA PROTEIN; FIMH PROTEIN; FLORFENICOL; GENTAMICIN; IHA PROTEIN; IUTA PROTEIN; NEOMYCIN; OUTER MEMBRANE PROTEIN; PROTEIN; QUINOLINE DERIVED ANTIINFECTIVE AGENT; SFA PROTEIN; SPECTINOMYCIN; STREPTOMYCIN; TETC PROTEIN; TETRACYCLINE; TRAT PROTEIN; UNCLASSIFIED DRUG; VEROTOXIN 2; YJA PROTEIN;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; AUSTRALIA; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; BLOOD GROUP TYPING; CONTROLLED STUDY; DIARRHEA; ENTEROTOXIGENIC ESCHERICHIA COLI; GENOTYPE; INTESTINE; MULTIDRUG RESISTANCE; NONHUMAN; PHENOTYPE; PLASMID; POLYMERASE CHAIN REACTION; SEROTYPE; SWINE; WEANING;

ANIMALS; AUSTRALIA; CLUSTER ANALYSIS; DIARRHEA; DNA, BACTERIAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ENTEROTOXIGENIC ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SWINE; SWINE DISEASES; VIRULENCE;

ESCHERICHIA COLI; SUIDAE; SUS;

EID: 77957273967     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2010.04.004     Document Type: Article

References (29)

1. Bischoff K.M., White D.G., McDermott P.F., Zhao S., Gaines S., Maurer J.J., Nisbe D.J. Characterization of chloramphenicol resistance in beta-hemolytic Escherichia coli associated with diarrhea in neonatal swine. Journal of Clinical Microbiology 2002, 40:389-394.
2. Bjornsson L., Hugenholtz P., Tyson G.W., Blackall L.L. Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removal. Microbiology 2002, 148:2309-2318.
3. Boerlin P., Travis R., Gyles C. Antimicrobial resistance and virulence genes of Escherichia coli isolates from swine in Ontario. Applied and Environmental Microbiology 2005, 71:6753-6761.
4. Boerlin P., White D.G. Antimicrobial resistance and its epidemiology. Antimicrobial Therapy in Veterinary Medicine 2006, 27-49. Blackwell Publishing, Stafford. S. Giguere, J.F. Prescott, J.D. Baggot, R.D. Walker, P.M. Dowling (Eds.).
5. Chapman T.A., Wu X., Barchia I., Bettelheim K.A., Driesen S., Trott D., Wilson M., Chin J. Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine. Applied and Environmental Microbiology 2006, 72:4782-4795.
6. CLSI Performance and Standards for Antimicrobial Disk Diffusion and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standards 2008, vol. 28, M31-A3 Edition. Clinical and Laboratory Standard Institute, Wayne, Pennsylvania, USA. 3rd edition.
7. Do N.T., Cu H.P., Nguyen N., Nguyen X.H., Au X.T., Van T.H., Vu N.Q., Trott D.J. Antimicrobial resistance phenotypes of ETEC isolates from piglets with diarrhea in North Vietnam. Annals of the New York Academy of Sciences 2006, 1081:543-545.
8. EAGAR Importance Ratings and Summary of Antibiotic Uses in Humans in Australia 2006, Expert Advisory Group on Antimicrobial Resistance, Canberra.
9. Fahy V.A., Connaughton I.D., Driesen S.J., Spicer E.M. Postweaning colibacillosis. Manipulating Pig Production 1987, 189-201. Australasian Pig Science Association, Werribee. J.L. Barnett, E.S. Batterham (Eds.).
10. Fairbrother J.M., Nadeau E., Gyles C.L. Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types pathogenesis and prevention strategies. Animal Health Research Reviews 2005, 6:17-39.
11. Goldstein C., Lee M.D., Sanchez S., Hudson C., Phillips B., Register B., Grady M., Liebert C., Summers A.O., White D.G., Maurer J.J. Incidence of class 1 and 2 integrases in clinical and commensal bacteria from livestock, companion animals, and exotics. Antimicrobial Agents and Chemotherapy 2001, 45:723-726.
12. Goswami P.S., Gyles C.L., Friendship R.M., Poppe C., Kozak G.K., Boerlin P. Effect of plasmid pTENT2 on severity of porcine post-weaning diarrhoea induced by an O149 enterotoxigenic Escherichia coli. Veterinary Microbiology 2008, 131:400-405.
13. Johnson J.A., Kuskowskia M.A., Owensa K., Clabotsa C., Singerd R.S. Virulence genotypes and phylogenetic background of fluoroquinolone-resistant and susceptible Escherichia coli urine isolates from dogs with urinary tract infection. Veterinary Microbiology 2008, 136:108-114.
14. Jordan D., Chin J., Fahy V. Antimicrobial use in the Australian pig industry: results of a national survey. Australian Veterinary Journal 2009, 87:222-229.
15. Kim E.H., Aoki T. Sequence analysis of the florfenicol resistance gene encoded in the transferable R-plasmid of a fish pathogen, Pasteurella piscicida. Microbiology and Immunology 1996, 40:665-669.
16. Maynard C., Fairbrother J.M., Bekal S., Sanschagrin F., Levesque R.C., Brousseau R., Masson L., Larivière S., Harel J. Antimicrobial resistance genes in enterotoxigenic Escherichia coli O149:K91 isolates obtained over a 23-year period from pigs. Antimicrobial Agents and Chemotherapy 2003, 47:3214-3221.
17. Ngeleka M., Pritchard J., Appleyard G., Middleton D.M., Fairbrother J.M. Isolation and association of Escherichia coli AIDA-I/STb, rather than EAST1 pathotype, with diarrhea in piglets and antibiotic sensitivity of isolates. Journal of Veterinary Diagnostic Investigation 2003, 15:242-252.
18. Olasz F., Fekete P.Z., Blum-Oehler G., Boldogkoi Z., Nagy B. Characterization of an F18+ enterotoxigenic Escherichia coli strain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC. FEMS Microbiology Letters 2005, 244:281-289.
19. Perez-Perez F.J., Hanson N.D. Detection of plasmid-mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR. Journal of Clinical Microbiology 2002, 40:2153-2162.
20. Pitout J.D.D., Hossain A., Hanson N.D. Phenotypic and molecular detection of CTX-M-β-lactamases produced by Escherichia coli and Klebsiella spp. Journal of Antimicrobial Chemotherapy 2004, 42. 5715-5121.
21. Radstrom P., Swedberg G., Skold O. Genetic analyses of sulfonamide resistance and its dissemination in gram-negative bacteria illustrate new aspects of R-plasmid evolution. Antimicrobial Agents and Chemotherapy 1991, 35:1840-1848.
22. Rosengren L.B., Waldner C.L., Reid-Smith R.J., Checkley S.L., McFall M.E., Rajić A. Antimicrobial resistance of fecal Escherichia coli isolated from grow-finish pigs in 20 herds in Alberta and Saskatchewan, Canada. Canadian Journal of Veterinary Research 2008, 72:160-167.
23. Saenz Y., Brinas L., Dominguez E., Ruiz J., Zarazaga M., Vila J., Torres C. Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains of human, animal and food origins. Antimicrobial Agents and Chemotherapy 2004, 48:3996-4001.
24. Schierack P., Walk N., Ewers C., Wilking H., Steinrück H., Filter M., Wieler L.H. ExPEC-typical virulence-associated genes correlate with successful colonization by intestinal E. coli in a small piglet group. Environmental Microbiology 2008, 10:1742-1751.
25. Schwarz S., Kehrenberg C., Doublet B., Cloeckaert A. Molecular basis of bacterial resistance to chloramphenicol and florfenicol. FEMS Microbiology Reviews 2004, 28:519-542.
26. Sidjabat H.E., Townsend K.M., Lorentzen M., Gobius K.S., Fegan N., Chin J., Bettelheim K.A., Hanson N.D., Bensink J.C., Trott D.J. Emergence and spread of two distinct clonal groups of multidrug-resistant Escherichia coli in a veterinary teaching hospital in Australia. Journal of Medical Microbiology 2006, 55:1125-1134.
27. Skurnik D., Ruimy R., Andremont A., Amorin C., Rouquet P., Picard B., Denamur E. Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli. Journal of Antimicrobial Chemotherapy 2006, 57:1215-1219.
28. Stokes H.W., Hall R.M. A novel family of potentially mobile DNA elements site-specific gene-integration functions: integrons. Molecular Microbiology 1989, 3:1669-1683.
29. White D.G., Hudson C., Maurer J.J., Ayers S., Zhao S., Lee M.D., L., B., Foley T., Sherwood J. Characterization of chloramphenicol and florfenicol resistance in Escherichia coli associated with bovine diarrhea. Journal of Clinical Microbiology 2000, 38:4593-4598.