Molecular cloning of canine excitatory amino acid transporter 5 and its detection in primary lens epithelial cells

Experimental Animals

Volumn 59, Issue 4, 2010, Pages 449-457

  Ochiai, Hideharu ^a   Saito, Miyoko ^a   Maruo, Takuya ^a   Kanemaki, Nobuyuki ^a  

^a AZABU UNIVERSITY   (Japan)

Author keywords

Amino acid transporter; Cdna sequence; Dog; Retina

Indexed keywords

EXCITATORY AMINO ACID TRANSPORTER 1; EXCITATORY AMINO ACID TRANSPORTER 2; EXCITATORY AMINO ACID TRANSPORTER 3; EXCITATORY AMINO ACID TRANSPORTER 4; EXCITATORY AMINO ACID TRANSPORTER 5; COMPLEMENTARY DNA; MESSENGER RNA;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CEREBELLUM; CONTROLLED STUDY; DOG; FEMALE; HUMAN VERSUS ANIMAL COMPARISON; LENS EPITHELIUM; MALE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; RETINA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; WESTERN BLOTTING; ANIMAL; CELL CULTURE; CHEMISTRY; EPITHELIUM CELL; GENE EXPRESSION; GENETICS; HUMAN; LENS; METABOLISM; MOLECULAR GENETICS; PROCEDURES; RAT; SPECIES DIFFERENCE;

CANIS FAMILIARIS; RATTUS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELLS, CULTURED; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DOGS; EPITHELIAL CELLS; EXCITATORY AMINO ACID TRANSPORTER 5; GENE EXPRESSION; HUMANS; LENS, CRYSTALLINE; MOLECULAR SEQUENCE DATA; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPECIES SPECIFICITY;

EID: 77957240033     PISSN: 13411357     EISSN: None     Source Type: Journal    
DOI: 10.1538/expanim.59.449     Document Type: Article

References (20)

1. Arriza, J., Eliasof, S., Kavanaugh, M.P., and Amara, S. 1997. Excitatory amino acid transorter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. U.S.A. 94: 4155-4160.
2. Bhat, S.P. 2001. The ocular lens epithelium. Biosci. Rep. 21: 537-563.
3. Boehmer, C., Rajamanickam, J., Schniepp, R., Kohler, K., Wulff, P., Kuhl, D., Palmada, M., and Lang, F. 2005. Regulation of the excitatory amino acid transporter EAAT5by the serum and glucocorticoid dependent kinases SKG1 and SKG3. Biochem. Biophys. Res. Commun. 329: 738-742.
4. Denker, B.M., Kwon, E.D., Kuhajda, F.P., and Agre, P. 1988. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 263: 15634-15642.
5. Fairman, W.A., Vandenberg, R.J., Arriza, J.L., Kavanaugh, M.P., and Amara, S.G. 1995. An excitatory amino-acid transporter with properties of a ligand-gated chloride channel. Nature 375: 599-603.
6. Fyk-Kolodziej, B., Qin, P., Dzhagaryan, A., and Pourcho, R.G. 2004. Differential cellular and subcellular distribution of glutamaters in the cat retina. Vis. Neurosci. 21: 551-565.
7. Harada, T., Harada, C., Nakamura, K., Quiah, H.A., Okumura, A., Namekata, K., Saeki, T., Aihara, M., Yoshida, H., Mitani, A., and Tanaka, K. 2007. The potential role of glutamate transporters in the pathogenesis of normal tension glaucoma. J. Clin. Inv. 117: 1763-1770.
8. Kanai, Y. and Hedger, M.A. 1992. Primary structure and functional characterization of a high-affinity glutamate transporter. Nature 360: 467-471.
9. Lim, J., Lam, Y.C., Kisler, J., and Donaldson, P.J. 2005. Molecular characterization of the cystine/glutamate exchanger and excitatory amino acid transporters in the rat lens. Inv. Ophthal. Vis. Sci. 46: 2869-2877.
10. Long, A.C., Aglar, A., Colitz, C.M., Zhang, J., Hayek, M.G., Failla, M.L., and Bomser, J.A.2008. Isolation and characterization of primary canine lens epithelial cells. Vet. Ophthal. 11: 38-42.
11. Maede, Y., Kasai, N., and Yaniguchi, N. 1982. Hereditary high concentration of glutathione in canine erythrocytes associated with high accumulation of glutamate, glutamine, and aspartate. Blood 59: 883-889.
12. Ochiai, H., Hishiyama, N., Hisamatsu, S., and Kanemaki, N. 2008. Aquaporin 1 expression in tissues of canines possessing inherited high K erythrocytes. J. Vet. Sci. 9: 203-205.
13. Pines, G., Danbolt, N.C., Bjoras, M., Zhang, Y., Bendahan, A., Eide, L., Koepsell, H., Storm, M.J., Seeberg, E., and Kannar, B.I. 1992. Cloning and expression of a rat brain L-glutamate transporter. Nature 360: 464-467.
14. Pow, D.V. and Barnett, N.L. 2000. Developmental expression of excitatory amino acid transporter 5: a photoreceptor and bipolar cell glutamate transporter in rat retina. Neurosci. Lett. 280: 21-24.
15. Pow, D.V., Barnett, N.L., and Penfold, P. 2000. Are neuronal transporters relavant in retinal glutamate homeostasis? Neurochem. Int. 37: 191-198.
16. Sato, K., Inaba, M., Suwa, Y., Matsuu, A., Hikasa, Y., Ono, K., and Kagota, K. 2000. Inherited defects of sodium- dependent glutamate transport mediated by glutamate/aspartate transporter in canine red cells due to a decreased level of transporter protein expression. J. Biol. Chem. 275: 6620-6627.
17. Sheng, M. 1996. PDZs and receptor/channel clustering: rounding up the latest suspects. Neuron 17: 575-578.
18. Slaughter, M.R., Thakkar, H., and O'Brien, P.J. 2003. Differential expression of the lenticular antioxidant system in laboratory animals: a determinant of species predilection to oxidative stress-induced ocular toxicity. Cur. Eye Res. 26: 15-23.
19. Storck, T., Schulte, S., Hoffman, K., and Stoffel, W. 1992. Structure, expression and functional analysis of a Na- dependent glutamate/aspartate transporter from rat brain. Proc. Natl. Acad. Sci. U.S.A. 89: 10955-10959.
20. Valencia, E., Marin, A., and Hardy, G. 2001. Glutathione- nutritional and pharmacological viewpoint. Nutrition 17: 485-486.