Purine restriction induces pronounced translational upregulation of the NT1 adenosine/pyrimidine nucleoside transporter in Leishmania major

Molecular Microbiology

Volumn 78, Issue 1, 2010, Pages 108-118

  Ortiz, Diana ^a   Valdés, Raquel ^a   Sanchez, Marco A ^a   Hayenga, Johanna ^a   Elya, Carolyn ^a   Detke, Siegfried ^b   Landfear, Scott M ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b UNIVERSITY OF NORTH DAKOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE; MESSENGER RNA; NUCLEOSIDE TRANSPORTER; NUCLEOSIDE TRANSPORTER 1; NUCLEOSIDE TRANSPORTER 2; NUCLEOSIDE TRANSPORTER 3; NUCLEOSIDE TRANSPORTER 4; PURINE; UNCLASSIFIED DRUG; URIDINE;

ARTICLE; CONTROLLED STUDY; GENE DELETION; LEISHMANIA MAJOR; NONHUMAN; PRIORITY JOURNAL; PROMASTIGOTE; PROTEIN EXPRESSION; PROTEIN METABOLISM; PURINE METABOLISM; RNA TRANSLATION; TRANSLATION REGULATION; UPREGULATION; WILD TYPE;

ADENOSINE; GENE DELETION; GENE EXPRESSION REGULATION; LEISHMANIA MAJOR; MEMBRANE TRANSPORT PROTEINS; MUTATION; PROTEIN BIOSYNTHESIS; PROTOZOAN PROTEINS; URIDINE;

LEISHMANIA MAJOR; PROTOZOA;

EID: 77957233776     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2010.07328.x     Document Type: Article

References (31)

1. Alleman, M.M. Gottlieb, M. (1996) Enhanced acquisition of purine nucleosides and nucleobases by purine-starved Crithidia luciliae. Mol Biochem Parasitol 76 : 279 287. (Pubitemid 26122741)
2. Carter, N.S., Drew, M.E., Sanchez, M., Vasudevan, G., Landfear, S.M. Ullman, B. (2000) Cloning of a novel inosine-guanosine transporter gene from Leishmania donovani by functional rescue of a transport-deficient mutant. J Biol Chem 275 : 20935 20941. (Pubitemid 30457690)
3. Carter, N.S., Yates, P., Arendt, C.S., Boitz, J.M. Ullman, B. (2008) Purine and pyrimidine metabolism in Leishmania. Adv Exp Med Biol 625 : 141 154.
4. Carter, N.S., Yates, P.A., Gessford, S.K., Galagan, S.R., Landfear, S.M. Ullman, B. (2010) Adaptive responses to purine starvation in Leishmania donovani. Mol Microbiol 78 : 92 107.
5. Clayton, C.E. (2002) Life without transcriptional control? From fly to man and back again. EMBO J 21 : 1881 1888.
6. Clayton, C. Shapira, M. (2007) Post-transcriptional regulation of gene expression in trypanosomes and leishmanias. Mol Biochem Parasitol 156 : 93 101. (Pubitemid 47600362)
7. Cohn, C.S. Gottlieb, M. (1997) The acquisition of purines by trypanosomatids. Parasitol Today 13 : 231 235. (Pubitemid 27263518)
8. Detke, S. (2007) TOR-induced resistance to toxic adenosine analogs in Leishmania brought about by the internalization and degradation of the adenosine permease. Exp Cell Res 313 : 1963 1978. (Pubitemid 46661845)
9. El-Sayed, N.M., Myler, P.J., Bartholomeu, D.C., Nilsson, D., Aggarwal, G., Tran, A.N., et al. (2005) The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science 309 : 409 415. (Pubitemid 40994675)
10. Gottlieb, M. (1985) Enzyme regulation in a trypanosomatid: effect of purine starvation on levels of 3′-nucleotidase activity. Science 227 : 72 74. (Pubitemid 15156220)
11. Goyard, S. Beverley, S.M. (2000) Blasticidin resistance: a new independent marker for stable transfection of Leishmania. Mol Biochem Parasitol 108 : 249 252. (Pubitemid 30317454)
12. Gueiros-Filho, F.J. Beverley, S.M. (1994) Loss-of-heterozygosity in Leishmania major: DHFR-TS as a negative marker. Woods Hole Molecular Parasitology Meeting Abstracts 5 : 133C.
13. Hall, S.T., Hillier, C.J. Gero, A.M. (1996) Crithidia luciliae: regulation of purine nucleoside transport by extracellular purine concentrations. Exp Parasitol 83 : 314 321.
14. Hall, S.T., Penny, J.I., Gero, A.M. Krishna, S. (1998) Crithidia luciliae: functional expression of nucleoside and nucleobase transporters in Xenopus laevis oocytes. Exp Parasitol 90 : 181 188. (Pubitemid 28471921)
15. Hammond, D.J. Gutteridge, W.E. (1984) Purine and pyrimidine metabolism in the Trypanosomatidae. Mol Biochem Parasitol 13 : 243 261.
16. Hassan, H.F. Coombs, G.H. (1986) A comparative study of the purine- and pyrimidine-metabolising enzymes of a range of trypanosomatids. Comp Biochem Physiol B 84 : 219 223.
17. de Koning, H.P., Watson, C.J., Sutcliffe, L. Jarvis, S.M. (2000) Differential regulation of nucleoside and nucleobase transporters in Crithidia fasciculata and Trypanosoma brucei brucei. Mol Biochem Parasitol 106 : 93 107. (Pubitemid 30089213)
18. Landfear, S.M., Ullman, B., Carter, N. Sanchez, M. (2004) Nucleoside and nucleobase transporters in parasitic protozoa. Eukaryotic Cell 3 : 245 254. (Pubitemid 38497964)
19. Ortiz, D., Sanchez, M.A., Pierce, S., Herrmann, T., Kimblin, N., Bouwer, H.G. Landfear, S.M. (2007) Molecular genetic analysis of purine nucleobase transport in Leishmania major. Mol Microbiol 64 : 1228 1243. (Pubitemid 46849014)
20. Ortiz, D., Sanchez, M.A., Koch, H.P., Larsson, H.P. Landfear, S.M. (2009a) An acid-activated nucleobase transporter from Leishmania major. J Biol Chem 284 : 16164 16169.
21. Ortiz, D., Sanchez, M.A., Quecke, P. Landfear, S.M. (2009b) Two novel nucleobase/pentamidine transporters from Trypanosoma brucei. Mol Biochem Parasitol 163 : 67 76.
22. Pfaffl, M.W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29 : e45.
23. Sacci, J.B., Jr., Campbell, T.A. Gottlieb, M. (1990) Leishmania donovani: regulated changes in the level of expression of the surface 3′-nucleotidase/nuclease. Exp Parasitol 71 : 158 168. (Pubitemid 20279486)
24. Sambrook, J., Fritsch, E.F. Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual.
25. Seyfang, A. Landfear, S.M. (1999) Substrate depletion upregulates uptake of myo-inositol, glucose and adenosine in Leishmania. Mol Biochem Parasitol 104 : 121 130. (Pubitemid 29518448)
26. Shi, W., Schramm, V.L. Almo, S.C. (1999) Nucleoside hydrolase from Leishmania major. Cloning, expression, catalytic properties, transition state inhibitors, and the 2.5-a crystal structure. J Biol Chem 274 : 21114 21120. (Pubitemid 129519617)
27. Stein, A., Vasudevan, G., Carter, N., Ullman, B., Landfear, S.M. Kavanaugh, M.P. (2003) Equilibrative nucleoside transporter family members from Leishmania donovani are electrogenic proton symporters. J Biol Chem 278 : 35127 35134. (Pubitemid 37102276)
28. Valdes, R.M., Arastu-Kapur, S.A., Landfear, S.M. Shinde, U. (2009) An Ab initio structural model of a nucleoside permease predicts functionally important residues. J Biol Chem 284 : 19067 19076.
29. Valdes, R., Vasudevan, G., Conklin, D. Landfear, S.M. (2004) Transmembrane domain 5 of the LdNT1.1 nucleoside transporter is an amphipathic helix that forms part of the nucleoside translocation pathway. Biochemistry 43 : 6793 6802. (Pubitemid 38697578)
30. Vasudevan, G., Carter, N.S., Drew, M.E., Beverley, S.M., Sanchez, M.A., Seyfang, A., et al. (1998) Cloning of Leishmania nucleoside transporter genes by rescue of a transport-deficient mutant. Proc Natl Acad Sci USA 95 : 9873 9878. (Pubitemid 28415262)
31. Walrad, P., Paterou, A., Acosta-Serrano, A. Matthews, K.R. (2009) Differential trypanosome surface coat regulation by a CCCH protein that co-associates with procyclin mRNA cis-elements. PLoS Pathog 5 : e1000317.