RNA-mediated gene silencing of FUT1 and FUT2 influences expression and activities of bovine and human fucosylated nucleolin and inhibits cell adhesion and proliferation

Journal of Cellular Biochemistry

Volumn 111, Issue 1, 2010, Pages 229-238

  Palumberi, Domenico ^a   Aldi, Silvia ^a   Ermini, Leonardo ^a   Ziche, Marina ^a   Finetti, Federica ^a   Donnini, Sandra ^a   Rosati, Floriana ^a  

^a UNIVERSITY OF SIENA   (Italy)

Author keywords

Fucosylglycoconjugates; Fucosyltransferases; Nucleolin; siRNA

Indexed keywords

ENDOSTATIN; FUCOSYLTRANSFERASE; NUCLEOLIN; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; BIOSYNTHESIS; CELL ADHESION; CELL GROWTH; CELL PROLIFERATION; CELL SURFACE; COW; DOWN REGULATION; FUCOSYLATION; GENE SILENCING; GROWTH INHIBITION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CATTLE; CELL ADHESION; CELL LINE; CELL PROLIFERATION; FUCOSE; FUCOSYLTRANSFERASES; GENE EXPRESSION REGULATION; GENE SILENCING; HUMANS; ISOENZYMES; PHOSPHOPROTEINS; PROTEIN ISOFORMS; RNA; RNA, SMALL INTERFERING; RNA-BINDING PROTEINS;

BOVINAE; MOLLUSCA;

EID: 77957151285     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.22692     Document Type: Article

References (42)

1. Abrantes J, Posada D, Guillon P, Esteves PJ, Le Pendu J. 2009. Widespread gene conversion of alpha-2-fucosyltransferase genes in mammals. J Mol Evol 69:22-31.
2. Aldi S, Della Giovampaola C, Focarelli R, Armini A, Ziche M, Finetti F, Rosati F. 2009. A fucose-containing O-glycoepitope on bovine and human nucleolin. Glycobiology 19:337-343.
3. Aubert M, Panicot L, Crotte C, Gibier P, Lombardo D, Sadoulet MO, Mas E. 2000. Restoration of alpha (1,2) fucosyltransferase activity decreases adhesive and metastatic properties of human pancreatic cancer cells. 60:1449-1456.
4. Bates PJ, Kahlon JB, Thomas SD, Trent JO, Miller DM. 1999. Antiproliferative activity of G-rich oligonucleotides correlates with protein binding. J Biol Chem 274:26369-26377.
5. Bourbon HM, Lapeyre B, Amalric F. 1988. Structure of the mouse nucleolin gene. The complete sequence reveals that each RNA binding domain is encoded by two independent exons. J Mol Biol 200:627-638.
6. Carpentier M, Morelle W, Coddeville B, Pons A, Masson M, Mazurier J, Legrand D. 2005. Nucleolin undergoes partial N- and O-glycosylations in the extranuclear cell compartment. Biochemistry 44:5804-5815.
7. Christian S, Pilch J, Akerman ME, Porkka K, Laakkonen P, Ruoslahti E. 2003. Nucleolin expressed at the cell surface is a marker of endothelial cells in angiogenic blood vessels. J Cell Biol 163:871-878. (Pubitemid 37517893)
8. Cordel S, Goupille C, Hallouin F, Meflah K, Le Pendu J. 2000. Role for alpha 1, 2- fucosyltransferase and histo-blood group antigen H type 2 in resistance of rat colon carcinoma cells to 5-fluorouracil. Int J Cancer 85:142-148.
9. Dapić V, Abdomerović V, Marrington R, Peberdy J, Rodger A, Trent JO, Bates PJ. 2003. Biophysical and biological properties of quadruplex oligodeoxyribonucleotides. Nucleic Acids Res 31:2097-2107.
10. Defize LH, Arndt-Jovin TM, Boonstra J, Meisenhelder J, Hunter T, de Hey H, de Last SW. 1988. A431 cell variants lacking the blood group A antigen display increased high affinity epidermal growth factor-receptor number, protein-tyrosine kinase activity, and receptor turnover. J Cell Biol 107:939-949.
11. Derenzini M, Sirri V, Trerè D, Ochs RL. 1995. The quantity of nucleolar proteins nucleolin and protein B23 is related to cell doubling time in human cancer cells. Lab Invest 73:497-502.
12. Focarelli R, Rosati F. 1995. The 220-kDa vitelline coat glycoprotein mediates sperm binding in the polarized egg of Unio elongatulus through O-linked oligosaccharides. Dev Biol 171:606-614.
13. Focarelli R, Capone A, Ermini L, Del Buono F, Battista La Sala G, Balasini M, Rosati F. 2003. Immunoglobulins against gp273, the ligand for sperm - egg interaction in the mollusc bivalve Unio elongatulus, are directed against charged O-linked oligosaccharide chains bearing a Lewis-like structure and interact with epitopes of the human zona pellucida. Mol Reprod Dev 64:226-234.
14. Ginisty H, Sicard H, Roger B, Bouvet P. 1999. Structure and functions of nucleolin. J Cell Sci 112:761-772.
15. Goupille C, Hallouin F, Meflah K, Le Pendu J. 1997. Increase of rat colon carcinoma cells tumorigenicity by alpha(1-2) fucosyltransferase gene transfection. Glycobiology 7:221-229.
16. Goupille C, Marionneau S, Bureau V, Hallouin F, Meichenin M, Rocher J, Le Pendu J. 2000. Alpha1,2 fucosyltransferase increases resistance to apoptosis of rat colon carcinoma cells. Glycobiology 10:375-382.
17. Hakomori SI. 1985. Aberrant glycosylation in cancer cell membranes as focused on glycolipids: Overview and perspectives. Cancer Res 45:2405-2414.
18. Harms G, Kraft R, Grelle G, Volz B, Dernedde J, Tauber R. 2001. Identification of nucleolin as a new L-selectin ligand. Biochem J 360:531-538. (Pubitemid 34020257)
19. Hovanessian AG, Puvion-Dutilleul F, Nisole S, Svab J, Perret E, Deng JS, Krust B. 2000. The cell-surface-expressed nucleolin is associated with the actin cytoskeleton. Exp Cell Res 261:312-328.
20. Iwamori M, Tanaka K, Kubushiro K, Lin B, Kiguchi K, Ishiwata I, Tsukazaki K, Nozawa S. 2005. Alterations in the glycolipid composition and cellular properties of ovarian carcinoma-derived RMG-1 cells on transfection of the alpha 1,2-fucosyltransferase gene. Cancer Sci 96: 26-30.
21. Legrand D, Vigié K, Said EA, Elass E, Masson M, Slomianny MC, Carpentier M, Briand JP, Mazurier J, Hovanessian AG. 2004. Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells. Eur J Biochem 271:303-317.
22. Losfeld ME, Khoury DE, Mariot P, Carpentier M, Krust B, Briand JP, Mazurier J, Hovanessian AG, Legrand D. 2009. The cell surface expressed nucleolin is a glycoprotein that triggers calcium entry into mammalian cells. Exp Cell Res 315:357-369.
23. Marionneau S, Bureau V, Goupille C, Hallouin F, Rocher J, Vaydie B, Le Pendu J. 2000. Susceptibility of rat colon carcinoma cells to lymphokine activated killer-mediated cytotoxicity is decreased by alpha 1,2-fucosylation. Int J Cancer 86:713-717.
24. Mejias-Luque R, Lopez-Ferrer A, Garrido M, Fabra A, de Bolos C. 2007. Changes in the invasive and metastatic capacities of HT-29/M3 cells induced by the expression of fucyltransferase 1. Cancer Sci 98:1000-1005.
25. Moheler TM, Saver S, Witzel M, Garcia-Vallejo JJ, Grobhotz R, Willhauck- Fleckenstein M, Greiner A, Goldschmidt H, Schartz-Albiez R. 2008. Involvement of alpha 1-2-fucosyltransferase I (FUT1) and surface expressed Lewis(y) in first endothelial cell - cell contacts during angiogenesis. J Cell Physiol 215:27-36.
26. Mongelard F, Bouvet P. 2007. Nucleolin: A multiFACeTed protein. Trends Cell Biol 17:80-86.
27. Monzavi-Karbassi B, Luo P, Jousheghany F, Torres-Quiñones M, Cunto- Amesty G, Artaud C, Kieber-Emmons T. 2004. A mimic of tumor rejection antigen-associated carbohydrates mediates an antitumor cellular response. Cancer Res 64:2162-2166.
28. Oriol R, Candelier JJ, Mollicone R. 2000. Molecular genetics of H. Vox Sang 78:105-118.
29. Orrick LR, Olson MO, Busch H. 1973. Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis Proc Natl Acad Sci USA 70:1316-1320.
30. Rozen S, Skaletsky HJ. 2000. Primer 3 on the www for general users and for biologist programmers. In: Krawetz S, Misener S, editors. Bioinformatics methods and protocols: Methods in molecular biology. Totowa, NY: Human Press. pp. 365-386.
31. Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG. 2002. The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor. J Biol Chem 277:37492-37502.
32. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning. A laboratory manual, 2nd edition. New York: Cold Spring Harbor Laboratory Press .
33. Schelling ME, Meininger CJ, Hawker JR, Jr., Granger HJ. 1988. Venular endothelial cells from bovine heart. Am J Physiol 254:H1211-H1217.
34. Semenkovich CF, Ostlund RE, Jr., Olson MO, Yang JW. 1990. A protein partially expressed on the surface of HepG2 cells that binds lipoproteins specifically is nucleolin. Biochemistry 29:9708-9713.
35. Shi H, Huang Y, Zhou H, Song X, Yuan S, Fu Y, Luo Y. 2007. Nucleolin is a receptor that mediates antiangiogenic and antitumor activity of endostatin. Blood 110:2899-2906.
36. Sirri V, Roussel P, Gendron MC, Hernandez-Verdun D. 1997. Amount of the two major Ag-NOR proteins, nucleolin, and protein B23 is cell-cycle dependent. Cytometry 28:147-156. (Pubitemid 27237144)
37. Srivastava M, Pollard HB. 1999. Molecular dissection of nucleolin's role in growth and cell proliferation: New insights. FASEB J 13:1911-1922.
38. Takagi M, Absalon MJ, McLure KG, Kastan MB. 2005. Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Cell 123:536-537.
39. Tissot B, North SJ, Ceroni A, Pang P, Panico M, Rosati F, Capone C, Haslam SM, Dell A, Morris HR. 2009. Glycoproteomics: Past, present and future. FEBS Lett 583:1728-1735.
40. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354. (Pubitemid 9256559)
41. Ugrinova I, Monier K, Ivaldi C, Thiry M, Storck S, Mongelard F, Bouvet P. 2007. Inactivation of nucleolin leads to nucleolar disruption, cell cycle arrest and defects in centrosome duplication. BMC Mol Biol 8:66.
42. Zhang Z, Sun P, Liu J, Fu L, Jan J, Liu Y, Yu L, Wang X, Yan Q. 2008. Suppression of FUT1/4 expression by siRNA inhibits tumor growth. Biochem Biophys Acta 1783:287-296.