ERK 1/2 and PI-3 kinase pathways as a potential mechanism of ghrelin action on cell proliferation and apoptosis in the porcine ovarian follicular cells

Journal of Physiology and Pharmacology

Volumn 61, Issue 4, 2010, Pages 451-458

  Rak Mardyla, A ^a   Gregoraszczuk, E L ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Apoptosis; Cell proliferation; Extracellular signal regulated protein kinase (ERK) 1 2; Ghrelin; Phosphoinositide 3 kinase; Pig ovarian cells

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; CASPASE 3; GHRELIN; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PHOSPHATIDYLINOSITOL 3 KINASE; WORTMANNIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL PROLIFERATION; COCULTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA FRAGMENTATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; NONHUMAN; OVARY FOLLICLE CELL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; SWINE; WESTERN BLOTTING;

ANIMALS; APOPTOSIS; CELL PROLIFERATION; CELLS, CULTURED; FEMALE; GHRELIN; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; OVARIAN FOLLICLE; PHOSPHATIDYLINOSITOL 3-KINASE; SWINE;

EID: 77957071242     PISSN: 08675910     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Fernandez-Fernandez R, Martini AC, Navarro V, et al. Novel signals for the integration of energy balance and reproduction. Mol Cell Endocrinol 2006; 254-255: 127-132.
2. Tena-Sempere M. Exploring the role of ghrelin as novel regulator of gonadal function. Growth Horm IGF Res 2005; 15(2): 83-88.
3. Kojima M, Hosada Y, Date M, Nakazato H, Matsuo H, Kangawa K. Ghrelin a growth hormone releasing acylated peptide from stomach. Nature 1999; 402: 656-660.
4. Howard AD, Feighner SD, Cully DF, et al. A receptor in pituitary and hypothalamus that functions in growth hormone release. Science 1996; 273: 974-977.
5. Gnanapavan S, Kola B, Bustin S, et al. The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans. J Clin Endocrinol Metab 2002; 87: 2988-2991.
6. Gaytan F, Barreiro ML, Chopin LK, et al. Immunolocalization of ghrelin and its functional receptor, the type 1a growth hormone secretagogue receptor, in the cyclic human ovary. J Clin Endocrinol Metab 2003; 88: 879-887.
7. Rak A, Gregoraszczuk EL. Ghrelin levels in prepubertal pig ovarian follicles. Acta Vet Hung 2009; 57: 109-113.
8. Rak A, Gregoraszczuk EL. Modulatory effect of ghrelin in prepubertal porcine ovarian follicles. J Physiol Pharmacol 2008; 59: 781-793.
9. Rak A, Szczepankiewicz D, Gregoraszczuk EL. Expression of ghrelin receptor, GHSR-1a, and its functional role in the porcine ovarian follicles. Growth Horm IGF Res 2009; 19: 68-76.
10. Dudek H, Datta SR, Franke TF, et al. Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 1997; 275(5300): 628-630.
11. Kulik G, Klippel A, Weber MJ. Antiapoptotic signalling by the insulin-like growth factor I receptor, phosphatidylinositol 3-kinase, and Akt. Mol Cell Biol 1997; 17: 1595-1606.
12. Makarevich A, Sirotkin A, Chrenek P, Bulla J, Hetenyi L. The role of IGF-I, cAMP/protein kinase A and MAP-kinase in the control of steroid secretion, cyclic nucleotide production, granulosa cell proliferation and preimplantation embryo development in rabbits. J Steroid Biochem Mol Biol 2000; 73: 123-133.
13. Makarevich AV, Sirotkin AV, Franek J, Kwon HB, Bulla J. The role of oxytocin, protein kinase A, and ERK-related MAP-kinase in the control of porcine ovarian follicle functions. Exp Clin Endocrinol Diabetes 2004; 112: 108-114.
14. van Opstal A, Boonstra J. Inhibitors of phosphatidylinositol 3-kinase activity prevent cell cycle progression and induce apoptosis at the M/G1 transition in CHO cells. Cell Mol Life Sci 2006; 63: 220-228.
15. Choi K, Auersperg N, Leung P. Mitogen-activated protein kinases in normal and (pre)neoplastic ovarian surface epithelium. Reprod Biol Endocrinol 2003; 1: 71.
16. Granata R, Settanni F, Biancone L, et al. Acylated and unacylated ghrelin promote proliferation and inhibit apoptosis of pancreatic beta-cells and human islets: involvement of 3',5'-cyclic adenosine monophosphate/protein kinase A, extracellular signal-regulated kinase 1/2, and phosphatidyl inositol 3-Kinase/Akt signaling. Endocrinology 2007; 148: 512-529.
17. Baldanzi G, Filigheddu N, Cutrupi S, et al. Ghrelin and desacyl ghrelin inhibit cell death in cardiomyocytes and endothelial cells through ERK1/2 and PI3-kinase/AKT. J Cell Biol 2003; 159: 29-37.
18. Kim S, Her S, Park SJ, et al. Ghrelin stimulates proliferation and differentiation and inhibits apoptosis in osteoblastic MC3T3-E1 cells. Bone 2005; 37: 359-369.
19. Nanzer A, Khalaf S, Mozid A, et al. Ghrelin exerts a proliferative effect on a rat pituitary somatotroph cell line via the mitogen-activated protein kinase pathway. Eur J Endocrinol 2004; 151: 233-240.
20. Andreis PG, Malendowicz L, Trejter M, et al. Ghrelin and growth hormone secretagogue receptor are expressed in the rat adrenal cortex: evidence that ghrelin stimulates the growth, but not the secretory activity of adrenal cells. FEBS Lett 2003; 536: 173-179.
21. Stoklosowa S, Bahr J, Gregoraszczuk E. Some morphological and functional characteristics of cells of the porcine theca interna in tissue culture. Biol Reprod 1978; 19: 712-719.
22. Stoklosowa S, Gregoraszczuk E, Channing CP. Estrogen and progesterone secretion by isolated cultured porcine theca and granulose cells. Biol Reprod 1982; 26: 943-952.
23. Alessi D, Cuenda A, Cohen P, Dudley D, Saltiel R. PD 098059 is a specific inhibitor of the activation of mitogenactivated protein kinase in vitro and in vivo. J Biol Chem 1995; 270: 27489-27494.
24. Dudley D, Pang L, Decker S, Bridges A, Saltiel A. A synthetic inhibitor of mitogen-activated protein kinase cascade. Proc Natl Acad Sci USA 1995; 92: 7686-7689.
25. Boulton S, Kyle S, Yalçintepe L, Durkacz B. Wortmannin is a potent inhibitor of DNA double strand break but not single strand break repair in Chinese hamster ovary cells. Carcinogenesis 1996; 17: 2285-2290.
26. Holleran J, Fourcade J, Egorin MJ, et al. In vitro metabolism of the phosphatidylinositol 3-kinase inhibitor, wortmannin, by carbonyl reductase. Drug Metab Dispos 2004; 32: 490-496.
27. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
28. Nicholson D, Ali A, Thornberry N, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 1995; 376: 37-43.
29. Zhang Y, Ying B, Shi L, et al. Ghrelin inhibit cell apoptosis in pancreatic beta cell line HIT-T15 via mitogen-activated protein kinase/phosphoinositide 3-kinase pathways. Toxicology 2007; 31: 194-202.
30. Kim M, Yoon C, Jang P, et al. The mitogenic and antiapoptotic actions of ghrelin in 3T3-L1 adipocytes. Mol Endocrinol 2004; 18: 2291-2301.
31. Delhanty P, van der Eerden B, van der Velde M, et al. Ghrelin and unacylated ghrelin stimulate human osteoblast growth via mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase (PI3K) pathways in the absence of GHS-R1a. J Endocrinol 2006; 188: 37-47.
32. Ahluwalia A, Li A, Cheng G, Deng X, Tarnawski AS. Reduced ghrelin in endothelial cells plays important mechanistic role in aging-related impairment of angiogenesis. J Physiol Pharmacol 2009; 60(2): 29-34.
33. Popelkova M, Sirotkin AV, Bezakova A, et al. Effect of IGFI, leptin, ghrelin and MAPK-ERK on the nuclear maturation of bovine oocytes. Bulletin Vet Res Inst Pulawy 2006; 50: 179-181.
34. Sirotkin A, Grossmann R. The role of ghrelin and some intracellular mechanisms in controlling the secretory activity of chicken ovarian cells. Comp Biochem Physiol A Mol Integr Physiol 2007; 147(1): 239-246.
35. Mousseaux D, Le Gallic L, Ryan J, et al. Regulation of ERK1/2 activity by ghrelin-activated growth hormone secretagogue receptor 1A involves a PLC/PKC epsilon pathway. Br J Pharmacol 2006; 148: 350-365.
36. Chung H, Kim E, Lee DH, et al. Ghrelin inhibits apoptosis in hypothalamic neuronal cells during oxygen-glucose deprivation. Endocrinology 2007; 148: 148-159.
37. Ceranowicz P, Warzecha Z, Dembinski A, et al. Treatment with ghrelin accelerates the healing of acetic acid-induced gastric and duodenal ulcers in rats. J Physiol Pharmacol 2009; 60: 87-98.
38. Graves L, Guy H, Kozlowski P, et al. Regulation of carbamoyl phosphate synthetase by MAP kinase. Nature 2000; 403: 328-332.
39. Downward J. PI 3-kinase, Akt and cell survival. Semin Cell Dev Biol 2004; 15: 177-182.
40. Fry M. Structure, regulation and function of phosphoinositide 3-kinases. Biochem Biophys Acta 1994; 1226: 237-268.
41. Camina J. Cell biology of the ghrelin receptor. J Neuroendocrinol 2006; 18: 65-76.
42. Cao JM, Ong H, Chen C. Effects of ghrelin and synthetic GH secretagogues on the cardiovascular system. Trends Endocrinol Metab 2006; 17: 13-18.
43. Van der Lely A, Tschop M, Heiman M, Ghigo E. Biological, physiological, pathophysiological and pharmacological aspects of ghrelin. Endocrinol Rev 2004; 25: 426-457.