Comparison of fluorescence in situ hybridisation using peptide nucleic acid probes, Gram stain/acridine orange leukocyte cytospin and differential time to positivity methods for detection of catheter-related bloodstream infection in patients after haematopoietic stem cell transplantation

Clinical Microbiology and Infection

Volumn 16, Issue 10, 2010, Pages 1591-1593

  Krause, R ^a   Salzer, H F ^a   Honigl M ^a   Valentin, T ^a   Auner, H W ^b   Zollner Schwetz, I ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACRIDINE ORANGE; PEPTIDE NUCLEIC ACID;

ADULT; AGED; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BLOOD CULTURE; CATHETER INFECTION; CENTRAL VENOUS CATHETER; CLINICAL ARTICLE; COAGULASE NEGATIVE STAPHYLOCOCCUS; COLONY FORMING UNIT; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; DIAGNOSTIC TEST; DIFFERENTIAL TIME TO POSITIVITY; ESCHERICHIA COLI; FEBRILE NEUTROPENIA; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; GRAM STAINING; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; INTERMETHOD COMPARISON; MALE; NUCLEIC ACID PROBE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS MITIS;

EID: 77957035784     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2010.03154.x     Document Type: Article

References (8)

1. Safdar N, Fine JP, Maki DG. Meta-analysis: methods for diagnosing intravascular device-related bloodstream infection. Ann Intern Med 2005, 142:451-466.
2. Mermel LA, Allon M, Bouza E. Clinical practice guidelines for the diagnosis and management of intravascular catheter-related infection: 2009 Update by the Infectious Diseases Society of America. Clin Infect Dis 2009, 49:1-45.
3. Kite P, Dobbins BM, Wilcox MH, McMahon MJ. Rapid diagnosis of central-venous-catheter-related bloodstream infection without catheter removal. Lancet 1999, 354:1504-1507.
4. Krause R, Auner HW, Gorkiewicz G. Detection of catheter-related bloodstream infections by the differential-time-to-positivity method and gram stain-acridine orange leukocyte cytospin test in neutropenic patients after hematopoietic stem cell transplantation. J Clin Microbiol 2004, 42:4835-4837.
5. Stender H. PNA FISH: an intelligent stain for rapid diagnosis of infectious diseases. Expert Rev Mol Diagn 2003, 3:649-655.
6. Malgrange VB, Escande MC, Theobald S. Validity of earlier positivity of central venous blood cultures in comparison with peripheral blood cultures for diagnosing catheter-related bacteremia in cancer patients. J Clin Microbiol 2001, 39:274-278.
7. Brun-Buisson C, Abrouk F, Legrand P, Huet Y, Larabi S, Rapin M. Diagnosis of central venous catheter-related sepsis. Critical level of quantitative tip cultures. Arch Intern Med 1987, 147:873-877.
8. Schwetz I, Hinrichs G, Reisinger EC, Krejs GJ, Olschewski H, Krause R. Delayed processing of blood samples influences time to positivity of blood cultures and results of Gram stain-acridine orange leukocyte Cytospin test. J Clin Microbiol 2007, 45:2691-2694.