Delayed processing of blood samples influences time to positivity of blood cultures and results of gram stain-acridine orange leukocyte cytospin test

Journal of Clinical Microbiology

Volumn 45, Issue 8, 2007, Pages 2691-2694

  Schwetz, I ^a   Hinrichs, G ^a   Reisinger, E C ^b   Krejs, G J ^a   Olschewski, H ^a   Krause, R ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACRIDINE ORANGE;

ARTICLE; BACTERIUM IDENTIFICATION; BLOOD ANALYSIS; BLOOD CULTURE; BLOOD SAMPLING; BLOOD STORAGE; CALCULATION; CRYOPRESERVATION; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; FUNGUS; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; GRAM STAIN ACRIDINE ORANGE LEUKOCYTE CYTOSPIN TEST; GRAM STAINING; NONHUMAN; PRIORITY JOURNAL; ROOM TEMPERATURE; TIME;

BACTEREMIA; BACTERIOLOGICAL TECHNIQUES; FALSE NEGATIVE REACTIONS; FALSE POSITIVE REACTIONS; HUMANS; SPECIMEN HANDLING; TEMPERATURE; TIME FACTORS;

EID: 34548102726     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00085-07     Document Type: Article

References (9)

1. Bjornson, H. S., R. Colley, R. H. Bower, V. P. Duty, J. T. Schwartz-Fulton, and J. E. Fischer. 1982. Association between microorganism growth at the catheter insertion site and colonization of the catheter in patients receiving total parenteral nutrition. Surgery 92:720-727.
2. Blot, F., G. Nitenberg, E. Chachaty, B. Raynard, N. Germann, S. Antoun, A. Laplanche, C. Brun-Buisson, and C. Tancrede. 1999. Diagnosis of catheter-related bacteraemia: a prospective comparison of the time to positivity of hub-blood versus peripheral-blood cultures. Lancet 354:1071-1077.
3. Brun-Buisson, C., F. Abrouk, P. Legrand, Y. Huet, S. Larabi, and M. Rapin. 1987. Diagnosis of central venous catheter-related sepsis. Critical level of quantitative tip cultures. Arch. Intern. Med. 147:873-877.
4. Cleri, D. J., M. L. Corrado, and S. J. Seligman. 1980. Quantitative culture of intravenous catheters and other intravascular inserts. J. Infect. Dis. 141:781-786.
5. Crump, J. A., and P. J. Collignon. 2000. Intravascular catheter-associated infections. Eur. J. Clin. Microbiol. Infect. Dis. 19:1-8.
6. Kite, P., B. M. Dobbins, M. H. Wilcox, and M. J. McMahon. 1999. Rapid diagnosis of central-venous-catheter-related bloodstream infection without catheter removal. Lancet 354:1504-1507.
7. Raad, I., H. A. Hanna, B. Alakech, I. Chatzinikolaou, M. M. Johnson, and J. Tarrand. 2004. Differential time to positivity: a useful method for diagnosing catheter-related bloodstream infections. Ann. Intern. Med. 140:18-25.
8. Raad, I. I., M. F. Sabbagh, K. H. Rand, and R. J. Sherertz. 1992. Quantitative tip culture methods and the diagnosis of central venous catheter-related infections. Diagn. Microbiol. Infect. Dis. 15:13-20.
9. Seifert, H., O. Cornely, K. Seggewiss, M. Decker, D. Stefanik, H. Wisplinghoff, and G. Fätkenheuer. 2003. Bloodstream infection in neutropenic cancer patients related to short-term nontunnelled catheters determined by quantitative blood cultures, differential time to positivity, and molecular epidemiological typing with pulsed-field gel electrophoresis. J. Clin. Microbiol. 41:118-123.