Identification of the 3C-protease-mediated 2A/2B and 2B/2C cleavage sites in the nonstructural polyprotein precursor of a dicistrovirus lacking the NPGP motif

Archives of Virology

Volumn 155, Issue 9, 2010, Pages 1477-1482

  Nakashima, Nobuhiko ^a   Ishibashi, Jun ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

3C PROTEASES; CYSTEINE PROTEINASE; POLYPROTEIN; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; DICISTROVIRIDAE; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN MOTIF; PROTEIN PROCESSING; SEQUENCE ALIGNMENT;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BASE SEQUENCE; CYSTEINE ENDOPEPTIDASES; DICISTROVIRIDAE; MOLECULAR SEQUENCE DATA; POLYPROTEINS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SEQUENCE ALIGNMENT; VIRAL NONSTRUCTURAL PROTEINS; VIRAL PROTEINS;

DICISTROVIRIDAE; PICORNAVIRIDAE; PLAUTIA STALI INTESTINE VIRUS;

EID: 77957018661     PISSN: 03048608     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00705-010-0723-z     Document Type: Article

References (18)

1. de Jong AS, de Mattia F, van Dommelen MM, Lanke K, Melchers WJ, Willems PH, van Kuppeveld FJ (2008) Functional analysis of picornavirus 2B proteins: effects on calcium homeostasis and intracellular protein trafficking. J Virol 82: 3782-3790.
2. Djikeng A, Kuzmickas R, Anderson NG, Spiro DJ (2009) Metagenomic analysis of RNA viruses in a fresh water lake. PloS One 4: e7264.
3. Gorbalenya AE, Koonin EV (1989) Viral proteins containing the purine NTP-binding sequence pattern. Nucleic Acids Res 17: 8413-8440.
4. Jan E (2006) Divergent IRES elements in invertebrates. Virus Res 119: 16-28.
5. Jones DT, Taylor WR, Thornton JM (1994) A model recognition approach to the prediction of all-helical membrane protein structure and topology. Biochemistry 33: 3038-3049.
6. Le Gall O, Christian P, Fauquet CM, King AM, Knowles NJ, Nakashima N, Stanway G, Gorbalenya AE (2008) Picornavirales, a proposed order of positive-sense single-stranded RNA viruses with a pseudo-T = 3 virion architecture. Arch Virol 153: 715-727.
7. Leong LEC, Cornell CT, Semler BL (2002) Processing determinants and functions of cleavage products of picornavirus polyproteins. In: Semler BL, Wimmer E (eds) Molecular biology of picornaviruses. ASM Press, Washington DC, pp 187-197.
8. Luke GA, de Felipe P, Lukashev A, Kallioinen SE, Bruno EA, Ryan MD (2008) Occurrence, function and evolutionary origins of '2A-like' sequences in virus genomes. J Gen Virol 89: 1036-1042.
9. Nakashima N, Nakamura Y (2008) Cleavage sites of the "P3 region" in the nonstructural polyprotein precursor of a dicistrovirus. Arch Virol 153: 1955-1960.
10. Nakashima N, Sasaki J, Tsuda K, Yasunaga C, Noda H (1998) Properties of a new picorna-like virus of the brown-winged green bug, Plautia stali. J Invertebr Pathol 71: 151-158.
11. Nakashima N, Shibuya N (2006) Multiple coding sequences for the genome-linked virus protein (VPg) in dicistroviruses. J Invertebr Pathol 92: 100-104.
12. Nakashima N, Uchiumi T (2009) Functional analysis of structural motifs in dicistroviruses. Virus Res 139: 137-147.
13. Rueckert RR, Wimmer E (1984) Systematic nomenclature of picornavirus proteins. J Virol 50: 957-959.
14. Sasaki J, Nakashima N, Saito H, Noda H (1998) An insect picorna-like virus, Plautia stali intestine virus, has genes of capsid proteins in the 3′ part of the genome. Virology 244: 50-58.
15. Skern T, Hampolz B, Guarne A, Fita I, Bergmann E, Petersen J, James MNG (2002) Structure and function of picornavirus proteinases. In: Semler BL, Wimmer E (eds) Molecular biology of picornaviruses. ASM Press, Washington DC, pp 199-212.
16. Terribilini M, Lee JH, Yan C, Jernigan RL, Honavar V, Dobbs D (2006) Prediction of RNA-binding sites in proteins from amino acid sequence. RNA 12: 1450-1462.
17. van Rij RP, Saleh MC, Berry B, Foo C, Houk A, Antoniewski C, Andino R (2006) The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Genes Dev 20: 2985-2995.
18. Wang XH, Aliyari R, Li WX, Li HW, Kim K, Carthew R, Atkinson P, Ding SW (2006) RNA interference directs innate immunity against viruses in adult Drosophila. Science 312: 452-454.