Optimization of podophyllotoxin extraction method from Linum album cell cultures

Pharmaceutical Biology

Volumn 48, Issue 12, 2010, Pages 1421-1425

  Yousefzadi, Morteza ^a   Sharifi, Mozafar ^a   Chashmi, Najmeh Ahmadian ^a   Behmanesh, Mehrdad ^a   Ghasempour, Alireza ^b  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b SHAHID BEHESHTI UNIVERSITY   (Iran)

Author keywords

Cell culture; Extraction; HPLC; Linum album; Podophyllotoxin

Indexed keywords

ALCOHOL; BUFFER; DICHLOROMETHANE; METHANOL; PODOPHYLLOTOXIN;

ACCURACY; ANGIOSPERM; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG ISOLATION; DRUG STRUCTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; INTERMETHOD COMPARISON; ISOLATION PROCEDURE; LINUM ALBUM; NONHUMAN; PHYTOTOXICITY; PLANT CELL CULTURE; PODOPHYLLUM; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; ULTRASOUND;

CELL CULTURE TECHNIQUES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLAX; METHANOL; METHYLENE CHLORIDE; PODOPHYLLOTOXIN; SOLVENTS; TIME FACTORS;

BERBERIDACEAE; LINACEAE; LINUM ALBUM; PODOPHYLLUM;

EID: 77956927893     PISSN: 13880209     EISSN: 17445116     Source Type: Journal    
DOI: 10.3109/13880209.2010.489564     Document Type: Article

References (16)

1. Baldi A, Srivastava AK, Bisaria VS. (2008). Improved podophyl-lotoxin production by transformed cultures of Linum album. Biotechnol J, 3, 1256-1263.
2. Canel C, Dayan FE, Canzera M, Khan IA, Rimando A, Burandt CL, Moraes RM. (2001). High yield of podophyllotoxin from leaves of Podophyllum peltatum by in situ conversion of podophyllotoxin 4-O-β-glucopyranoside. Planta Med, 67, 97-99.
3. Farkya S, Bisaria VS, Srivastava AK. (2004). Biotechnological aspects of the production of the anticancer drug podophyllotoxin. Appl Microbiol Biotechnol 65: 504-519.
4. Fuss E (2003): Lignans in plant cell and organ cultures: An overview. Phytochem Rev, 2, 307-320.
5. Chattopadhyay SA, Bisaria VS, Srivastava AK. (2004). Cytotoxicity of in vitro produced podophyllotoxin from Podophyllum hexandrum on human cancer cell line. Nat Prod Res, 18, 51-57.
6. Izadifar M, Baik OD. (2008). An optimum ethanol-water solvent system for extraction of podophyllotoxin: Experimental study, difusivity determination and modeling. Sep Purif Technol, 63, 53-60.
7. Meagher LP, Beecher GR, Flanagan VP, Li BV. (1999). Isolation and characterization of the lignans, isolariciresinol and pinoresinol in faxseed. J Agric Food Chem, 47, 31173-31180.
8. Kartal M, Konuklugil B, Indrayanto G, Alfermann AW. (2004). Comparison of diferent extraction methods for the determination of podophyllotoxin and 6-methoxypodophyllotoxin in Linum species. J Pharm Biomed Anal, 35, 441-447.
9. Pandey H, Nandi SK, Kumar A, Palni UT, Palni S. (2007). Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altitude. Acta Physiol Plant, 29, 121-126.
10. Petersen M, Alfermann AW. (2001). Te production of cytotoxic lignans by plant cell cultures. Appl Microbiol Biotechnol, 55, 135-142.
11. Seidel V, Windhovel J, Eaton G, Alfermann AW, Arroo RJ, Medarde M, Petersen M, Wolley JG. (2002). Biosynthesis of podophyllotoxyn in Linum album cell culture. Planta, 215, 1031-1039.
12. Sharifnia F, Assadi M. (2001). Flora of Iran; Linaceae, No. 34. Tehran: Research Institute of Forests and Rangelands.
13. Smonlly T, Wichers H, Kalenberg S, Shahsavari A, Peterson M, Alferman AW. (1998). Accumulation of podophyllotoxin and related lignans cell suspension of Linum album. Phytochemistry, 48, 975-979.
14. Van Uden W, Pras N, Visser J F, Malingre M. (1989). Detection and iden-tifcation of podophyllotoxin produced by cell cultures derived from Podophyllum hexandrum Royle. Plant Cell Rep, 8, 165-168.
15. Westcott ND, Muir AD. (1998). Process for extraction of lignans from faxseed. United States Patent 5705618.
16. Zhang ZS, Li D, Wang LJ, Ozkanc N, Chena ZD, Maoa ZH, Yang H. (2007). Optimization of ethanol water extraction of lignans from faxseed. Sep Purif Technol, 57, 17-24.