Purification of recombinant growth hormone by clear native gels for conformational analyses: Preservation of conformation and receptor binding

Amino Acids

Volumn 39, Issue 3, 2010, Pages 859-869

  Chen, Wei Qiang ^a   Salmazo, Anita ^b   Myllykoski, Matti ^c   Sjöblom, Björn ^b   Bidlingmaier, Martin ^d   Pollak, Arnold ^a   Baumgärtel, Peter ^e   Djinovic Carugo, Kristina ^f   Kursula, Petri ^c,g   Lubec, Gert ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF VIENNA   (Austria)

^c UNIVERSITY OF OULU   (Finland)

^d LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^e HAHN MEITNER INSTITUT   (Germany)

^f UNIVERSITY OF LJUBLJANA   (Slovenia)

^g DESY   (Germany)

Author keywords

Conformation; Electroelution; Function; Growth hormone; SRCD

Indexed keywords

HUMAN GROWTH HORMONE; GROWTH HORMONE; GROWTH HORMONE RECEPTOR; PROTEIN BINDING; RECOMBINANT PROTEIN;

ARTICLE; CIRCULAR DICHROISM; CLEAR NATIVE GEL ELECTROPHORESIS; CONTROLLED STUDY; COOLING; CRYSTAL STRUCTURE; ELECTROPHORESIS; ELUTION; GEL ELECTROPHORESIS; HORMONE RECEPTOR INTERACTION; MELTING POINT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION; RECEPTOR BINDING; STEREOCHEMISTRY; STRUCTURE ANALYSIS; SYNCHROTRON RADIATION; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; CHEMISTRY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GROWTH HORMONE; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; RECEPTORS, SOMATOTROPIN; RECOMBINANT PROTEINS;

EID: 77956904257     PISSN: 09394451     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00726-010-0542-2     Document Type: Article

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