Improving efficiency of a small forensic DNA laboratory: Validation of robotic assays and evaluation of microcapillary array device

Croatian Medical Journal

Volumn 46, Issue 4, 2005, Pages 563-577

  Crouse, Cecelia ^a   Yeung, Stephanie ^b   Greenspoon, Susan ^c   McGuckian, Amy ^a   Sikorsky, Julie ^a   Ban, Jeff ^c   Mathies, Richard ^b  

^a Palm Beach County Sheriff's Office   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Virginia Division of Forensic Science   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CAPILLARY ELECTROPHORESIS; DEVICE; DNA DETERMINATION; DNA EXTRACTION; DNA FINGERPRINTING; FORENSIC MEDICINE; LABORATORY; MICROCAPILLARY ARRAY DEVICE; REAL TIME POLYMERASE CHAIN REACTION; ROBOTICS; SHORT TANDEM REPEAT;

EID: 24344466396     PISSN: 03539504     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. United States Department of Justice, Bureau of Justice Statistics Bulletin. Census of publicly funded forensic crime laboratories, February 2005 NCJ 207205. Available at: http://www.ojp.usdoj.gov/bis/whtsnw2.htm. Accessed: April 20, 2005.
2. Forensic Science Communications. Quality assurance standards for forensic DNA testing laboratories. Vol 2. 2000. Available at: http://www.fbi.gov/hq/lab/ fsc/back issu/july2000/codispre.htm. Accessed: July 7, 2005.
3. Federal Bureau of Investigation. Quality assurance standards for forensic DNA testing laboratories. October, 1998. Available at: http://www.fbi.gov/ congress/congress 02/forensicstd.htm. Accessed: July 7, 2005.
4. Sambrook J, Fritsch EF, Maniatis T. Bacteriophage lambda growth, purification and DNA extraction. In: Sambrook J, Fritsch EF, Maniatis T, editors. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1989. vol. 1. p. 2.60-2.81.
5. Greenspoon S, Ban J. Robotic extraction of mock sexual assault samples using the Biomek® 2000 and the DNA IQ™ System. Profiles in DNA. 2002;5:3-5.
6. Promega's DNA IQ* DNA isolation system automated on the Beckman Coulter Biomek® 2000 Laboratory Workstation. Available at: http://www.beckman.com/resourcecenter/eLabNotebook/ForensicID/dna-iq-bmk 2000/index.htm. Accessed: April 20, 2005.
7. Promega Corporation: DNA IQ™ System-Small Sample casework Protocol. Available at: http://www.promega. com. Accessed: July 7, 2005.
8. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988;239:487-91.
9. Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 1986;(51 Pt 1):263-73.
10. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5′>3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci U S A. 1991;88:7276-80.
11. Kline MC, Duewer DL, Redman JW, Butler JM. NIST Mixed Stain Study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity. Anal Chem. 2003;75:2463-9.
12. Nicklas JA, Buel E. Development of an Alu-based, real-time PCR method for quantitation of human DNA in forensic samples. J Forensic Sci. 2003;48:936-44.
13. Tomsey CS, Kurtz M, Kist F, Hockensmith M, Call P. Comparison of PowerPlex 16, PowerPlex1.1/2.1, and ABI AmpfISTR Profiler Plus/COfiler for forensic use. Croat Med J. 2001;42:239-43.
14. Krenke BE, Tereba A, Anderson SJ, Buel E, Culhane S, Finis CJ, et al. Validation of a 16-locus fluorescent multiplex system. J Forensic Sci. 2002;47:773-85.
15. Finis C. Megaplex STR analysis from a single amplification: validation of the PowerPlex® 16 system. Profiles in DNA. 2001;4:3-6.
16. Greenspoon SA, Ban JD, Pablo L, Grouse CA, Kist FG, Tomsey CS, et al. Validation and implementation of the PowerPlex 16 BIO System STR multiplex for forensic casework. J Forensic Sci. 2004;49:71-80.
17. Sutherland JC, Sutherland BM, Emrick A, Monteleone DC, Ribeiro EA, Trunk J, et al. Quantitative electronic imaging of gel fluorescence with CCD cameras: applications in molecular biology. Biotechniques. 1991;10: 492-7.
18. Edwards K, Logan J, Saunders N. Real-time PCR: an essential guide. Norfolk (UK): Horizon Bioscience; 2004.
19. Bernard PS, Wittwer CT. Real-time PCR technology for cancer diagnostics. Clin Chem. 2002;48:1178-85.
20. Higuchi R, Fockler C, Dollinger G, Watson R. Kinetic PCR Analysis: Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology (NY). 1993;11:1026-30.
21. Hayn S, Wallace MM, Prinz M, Shaler RC. Evaluation of an automated liquid hybridization method for DNA quantitation. J Forensic Sci. 2004;49:87-91.
22. Kline MC, Duewer DL, Redman JW, Butler JM, Boyer DA. Polymerase chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the "suitability for purpose" of four filter papers as archival media. Anal Chem. 2002;74:1863-9.
23. Richard ML, Frappier RH, Newman JC. Developmental validation of a real-time quantitative PCR assay for automated quantification of human DNA. J Forensic Sci. 2003;48:1041-6.
24. Rutledge RG, Cote C. Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res. 2003;31:e93.
25. Butler J, Vallone P. High-throughput genetic analysis through multiplexed PCR and multicapillary electrophoresis. In: Weissensteiner T, Griffin HG, Griffin A, editors. PCR Technologies: Current Innovations. 2nd ed. Boca Raton (FL): CRC Press; 2004. p. 111-20.
26. Paegel BM, Blazej RG, Mathies RA. Microfluidic devices for DNA sequencing: sample preparation and electrophoretic analysis. Curr Opin Biotechnol. 2003; 14:42-50.
27. Berti L, Medintz IL, Tom J, Mathies RA. Energy-transfer cassette labeling for capillary array electrophoresis short tandem repeat DNA fragment sizing. Bioconjug Chem. 2001;12:493-500.
28. Applied BioSystems. Quantifiler human DNA quantification kit and quantifiler Y human male DNA quantification Kit. User's manual. Available at: http://docs. appliedbiosystems.com/pebiodocs/04344790.pdf. Accessed: April 20, 2005.
29. PowerPlex® 16 BIO system. Technical manual. Madison (WI): Promega Corporation; 2001.
30. Gill P, Kirkham A. Development of a simulation model to assess the impact of contamination in casework using STRs. J Forensic Sci. 2004;49:485-91.
31. Kloosterman AD, Kersbergen P. Efficacy and limits of genotyping low copy number (LCN) DNA samples by multiplex PCR of STR loci. J Soc Biol. 2003;197:351-9.
32. Applied Biosystems. Quantifiler user's manual. Foster City (CA): AB; 2004.
33. Schoske R, Vallone PM, Ruitberg CM, Butler JM. Multiplex PCR design strategy used for the simultaneous amplification of 10 Y chromosome short tandem repeat (STR) loci. Anal Bioanal Chem. 2003;375:333-43.
34. Butler JM, Schoske R, Vallone PM. Highly multiplexed assays for measuring polymorphisms on the Y-chromosome. In: Brinkmann B, Carracedo A, editors. Progress in Forensic Genetics 9. Amsterdam: Elsevier Science, International Congress Series 1239; 2003. p. 301-5.