Immiscible phase nucleic acid purification eliminates PCR inhibitors with a single pass of paramagnetic particles through a hydrophobic liquid

Journal of Molecular Diagnostics

Volumn 12, Issue 5, 2010, Pages 620-628

  Sur, Kunal ^a   McFall, Sally M ^a   Yeh, Emilie T ^a   Jangam, Sujit R ^a   Hayden, Mark A ^b   Stroupe, Stephen D ^b   Kelso, David M ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b Abbott Molecular Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; GENOMIC DNA; MAGNETIC NANOPARTICLE; VIRUS RNA;

AQUEOUS SOLUTION; ARTICLE; CHLAMYDIA TRACHOMATIS; DNA PURIFICATION; HUMAN IMMUNODEFICIENCY VIRUS 1; HYDROPHOBICITY; NEISSERIA GONORRHOEAE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; URINE; VIRUS DETECTION;

EID: 77956852246     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.2353/jmoldx.2010.090190     Document Type: Article

References (30)

1. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J: Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990, 28:495-503 (Pubitemid 20078123)
2. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162:156-159
3. Papell SS, inventor; United State of America as Represented by the Administrator of the National Aeronautics and Space Administration, assignee. Low viscosity magnetic fluid obtained by the colloidal suspension of magnetic particles. United States patent US 3,215,572.1965 Nov
4. Reimers GW, Khalafalla SE, inventors; United States of America as Represented by the Secretary of the Interior, assignee. Production of magnetic fluids by petization techniques. United States patent US 3,843,540. 1974 Oct
5. Landfester K, Ramírez LP: Encapsulated magnetite particles for biomedical application. J Phys Condens Matter 2003, 15:S1345-S1361
6. Molday RS, Mackenzie D: Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells. J Immunol Methods 1982, 52:353
7. Sangregorio C, Wiemann JK, O'Connor CJ, Rosenzweig Z: A new method for the synthesis of magnetoliposomes. J Appl Phys 1999, 85:5699
8. Pardoe H, Chua-anusorn W, St. Pierre TG, Dobson J: Structural and magnetic properties of nanoscale iron oxide particles synthesized in the presence of dextran or polyvinyl alcohol. J Magn Magn Mater 2001, 225:41-46
9. Rembaum A, inventor; California Institute of Technology, assignee. Polyglutaraldehyde synthesis and protein bonding substrates. United States patent US 4,369,226. 1983
10. Lee J, Isobe T, Senna M: Magnetic properties of ultrafine magnetite particles and their slurries prepared via in-situ precipitation. Colloids Surf A Physicochem Eng Asp 1996, 109:121-127 (Pubitemid 26157117)
11. Amellal B, Murphy R, Maiga A, Brucker G, Katlama C, Calvez V, Marcelin AG: Stability of HIV RNA in plasma specimens stored at different temperatures. HIV Med 2008, 9:790-793
12. Swanson P, Holzmayer V, Huang S, Hay P, Adebiyi A, Rice P, Abravaya K, Thamm S, Devare SG, Hackett JJ: Performance of the automated Abbott RealTime HIV-1 assay on a genetically diverse panel of specimens from London: comparison to VERSANT HIV-1 RNA 3.0, AMPLICOR HIV-1 MONITOR v1.5, and LCx HIV RNA Quantitative assays. J Virol Methods 2006, 137:184-192
13. Erickson D, Li D: Integrated microfluidic devices. Anal Chim Acta 2004, 507:11-26
14. Mitchell P: Microfluidics-downsizing large-scale biology. Nature Biotechnol 2001, 19:717-721
15. Easley CJ, Karlinsey JM, Bienvenue JM, Legendre LA, Roper MG, Feldman SH, Hughes MA, Hewlett EL, Merkel TJ, Ferrance JP, Landers JP: A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability. Proc Natl Acad Sci USA 2006, 103:19272-19277
16. Claassen M, van Zyl GU, Preiser W: Extraction buffer contaminated bacterially as a cause of invalid HIV-1 viral load results on the NucliSens EasyQ® system. J Virol Meth 2008, 150:80-81
17. Berensmeier S: Magnetic particles for the separation and purification of nucleic acids. Appl Microbiol Biotechnol 2006, 73:495-504
18. Fang X, Willis RC, Burrell A, Evans K, Hoang Q, Xu W, Bounpheng M: Automation of nucleic acid isolation on kingfisher magnetic particle processors. J Assoc Lab Automat 2007, 12:195-201
19. Huang S, Salituro J, Tang N, Luk K-C, Hackett J Jr, Swanson P, Cloherty G, Mak W-B, Robinson J, Abravaya K: Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucl Acids Res 2007, 35:e101
20. Folks TM, Powell D, Lightfoote M, Koenig S, Fauci AS, Benn S, Rabson A, Daugherty D, Gendelman HE, Hoggan MD, Venkatesan S, Martin MA: Biological and biochemical characterization of a cloned Leu-3-cell surviving infection with the acquired immune deficiency syndrome retrovirus. J Exp Med 1986, 164:280-290 (Pubitemid 16069238)
21. Marshall R, Chernesky M, Jang D, Hook EW, Cartwright CP, Howell-Adams B, Ho S, Welk J, Lai-Zhang J, Brashear J: Characteristics of the m2000 automated sample preparation and multiplex real-time PCR System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol 2007, 45:747-751
22. Gundling G: Nucleic acid isolation method and kit. Abbott Laboratories; 2005
23. Latham G, Fang X, Conrad R, Kemppainen J, Setterquist R, Pasloske B: Modified surfaces as solid supports for nucleic acid purification. USPTO, Ambion Inc., 2005
24. Nargessi, RD, Pourfarzaneh, M, inventors; Cortex Biochem, Inc., assignee. Isolation and purification of nucleic acids. United States patent US 7,264,927. 2007 Sep. 4
25. Mylonakis E, Paliou M, Rich J: Plasma viral load testing in the management of HIV infection. Am Fam Physician 2001, 63:483-490
26. Dineva MA, Mahilum-Tapay L, Lee H: Sample preparation: a challenge in the development of point-of-care nucleic acid-based assays for resource-limited settings. Analyst 2007, 132:1193-1199 (Pubitemid 350145319)
27. Jagodzinski LL, Wiggins DL, McManis JL, Emery S, Overbaugh J, Robb M, Bodrug S, Michael NL: Use of calibrated viral load standards for group M subtypes of human immunodeficiency virus type 1 to assess the performance of viral RNA quantitation tests. J Clin Microbiol 2000, 38:1247-1249
28. Swanson P, Huang S, Holzmayer V, Bodelle P, Yamaguchi J, Brennan C, Badaro R, Brites C, Abravaya K, Devare SG: Performance of the automated Abbott RealTime HIV-1 assay on a genetically diverse panel of specimens from Brazil. J Virol Methods 2006, 134:237-243
29. Read JS, Committee on Pediatric AIDS, American Academy of Pediatrics: Diagnosis of HIV-1 infection in children younger than 18 months in the United States. Pediatrics 2007, 120:e1547-e1562
30. Fiscus SA, Cheng B, Crowe SM, Demeter L, Jennings C, Miller V, Respess R, Stevens W: Forum for Collaborative HIV Research Alternative Viral Load Assay Working Group: HIV-1 viral load assays for resource-limited settings. PLoS Med 2006, 3:e47