Comparison of XTT and Alamar blue assays in the assessment of the viability of various human cancer cell lines by AT-101 (-/- gossypol)

Toxicology Mechanisms and Methods

Volumn 20, Issue 8, 2010, Pages 482-486

  Uzunoglu, Selim ^a   Karaca, Burcak ^b   Atmaca, Harika ^a   Kisim, Asli ^a   Sezgin, Canfeza ^b   Karabulut, Bulent ^b   Uslu, Ruchan ^b  

^a CELAL BAYAR UNIVERSITY   (Turkey)

^b EGE UNIVERSITY   (Turkey)

Author keywords

Alamar blue assay; AT 101; Human cancer cells; Viability; XTT assay

Indexed keywords

2,3 BIS (2 METHOXY 4 NITRO 5 SULFOPHENYL) 5 [(PHENYLAMINO)CARBONYL] 2H TETRAZOLIUM HYDROXIDE; ALAMAR BLUE; DYE; GOSSYPOL; ISOSORBIDE; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; CANCER CELL CULTURE; CELL STRAIN MCF 7; CELL VIABILITY; COMPARATIVE STUDY; CONTROLLED STUDY; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; FEMALE; GOSSYPOL; HUMANS; INDICATORS AND REAGENTS; MALE; NEOPLASMS; OXAZINES; REPRODUCIBILITY OF RESULTS; TETRAZOLIUM SALTS; TUMOR STEM CELL ASSAY; XANTHENES;

GOSSYPIUM HIRSUTUM;

EID: 77956843545     PISSN: 15376516     EISSN: 15376524     Source Type: Journal    
DOI: 10.3109/15376516.2010.508080     Document Type: Article

References (18)

1. Ahmed SA, Gogal RM, Walsh JE. 1994. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J Immunol Methods 170:211-224.
2. Band V, Hoffer AP, Band H, Rhinehardt AE, Knapp RC, Matlin SA, Anderson DC. 1989. Antiproliferative effect of gossypol and its optical isomers on human reproductive cancer cell lines. Gynecol Oncol 32:273-277.
3. Buttke TM, McCubrey JA, Owen TC. 1993. Use of an aqueous soluble tetrazolium/ formazan assay to measure viability and proliferation of lymphokine- dependent cell lines. J Immunol Methods 157:233-240.
4. Coutinho EM. 2002. Gossypol: a contraceptive for men. Contraception 65:259-263.
5. De Fries R, Mitsuhashi M. 1995. Quantification of mitogen induced human lymphocyte proliferation: comparison of the Alamar blue assay to 3Hthymidine incorporation assay. J Clin Lab Anal 9:89-95.
6. Debaun RM, De Stevens G. 1951. On the mechanism of enzyme action. XLIV. Codetermination of resazurin and resorufin in enzymatic dehydrogenation experiments. Arch Biochem 31:300-308.
7. Dodou K. 2005. Investigations on gossypol: past and present developments. Expert Opin Investig Drugs 14:1419-1434.
8. Ishyiama MSM, Sasamoto K, Mizoguchi M, He P. 1993. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem Pharm Bull 41:1118-1122.
9. Larson EM, Doughman DJ, Gregerson DS, Obritsch WF. 1997. A new, simple, non-radioactive, non-toxic in vitro assay to monitor corneal endothelial cell viability. Invest Ophthalmol Vis Sci 38:1929-1933.
10. Meshulam T, Levitz SM, Christin L, Diamond RD. 1995. A simplified new assay for assessment of fungal cell damage with the tetrazolium dye, (2,3)-bis- (2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanil ide (XTT). J Infect Dis 172:1153-1156.
11. Nakayama GR, Caton MC, Nova MP, Parandoosh Z. 1997. Assessment of the Alamar blue assay for cellular growth and viability in vitro. J Immunol Methods 204:205-208.
12. Page B, Page M, Noël C. 1993. A new fluorometric assay for cytotoxicity measurements in vitro. Int J Oncol 3:473-476.
13. Roehm NW, Rodgers GH, Hatfield SM, Glasebrook AL. 1991. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J Immunol Methods 142:257-265.
14. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Thierney S, Nofziger TH, Currens MJ, Seniff D, Boyd MR. 1988. Evaluation of a soluble tetrazolium/ formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 48:4827-4833.
15. Shahan TA, Siegel PD, Sorenson WG, Kuschner WG, Lewis DM. 1994. A sensitive new bioassay for tumor necrosis factor. J Immunol Methods 175:181-187.
16. Tuszynski GP, Cossu G. 1984. Differential cytotoxic effect of gossypol on human melanoma, colon carcinoma and other tissue culture cell lines. Cancer Res 44:768-771.
17. Voytik-Harbin SL, Brightman AO, Waisner B, Lamar CH, Badylak SF. 1998. Application and evaluation of the Alamar blue assay for cell growth and survival of fibroblasts. In Vitro Cell Dev Biol Anim 34:239-246.
18. White MJ, DiCaprio MJ, Greenberg DA. 1996. Assessment of neuronal viability with Alamar blue in cortical and granule cell cultures. J Neurosci Methods 70:195-200.