Assessment of neuronal viability with Alamar blue in cortical and granule cell cultures

Journal of Neuroscience Methods

Volumn 70, Issue 2, 1996, Pages 195-200

  White, Melissa J ^a   DiCaprio, Michael J ^a   Greenberg, David A ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Alamar blue; apoptosis; cell culture; cerebellar granule cell; cortical neuron; excitotoxicity; hypoxia; neuronal death

Indexed keywords

DYE; EXCITOTOXIN; GLUTAMIC ACID; N METHYL DEXTRO ASPARTIC ACID;

ANIMAL CELL; APOPTOSIS; ARTICLE; BRAIN CORTEX; CELL CULTURE; CELL VIABILITY; CEREBELLUM; CONTROLLED STUDY; EMBRYO; GRANULE CELL; HYPOXIA; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CELL HYPOXIA; CELL SURVIVAL; CELLS, CULTURED; CEREBELLUM; CEREBRAL CORTEX; COLORING AGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; EXCITATORY AMINO ACID AGONISTS; GLUCOSE; GLUTAMIC ACID; L-LACTATE DEHYDROGENASE; N-METHYLASPARTATE; NEURONS; NEUROTOXINS; NITROPRUSSIDE; OXAZINES; POTASSIUM; RATS; RATS, SPRAGUE-DAWLEY; VASODILATOR AGENTS; XANTHENES;

EID: 0030605279     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(96)00118-5     Document Type: Article

References (18)

1. Bonfoco, E., Krainc, D., Ankarcrona, M., Nicotera, P. and Lipton, S.A. (1995) Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures, Proc. Natl. Acad. Sci. USA, 92: 7162-7166.
2. D'Mello, S.R., Galli, C., Ciotti, T. and Calissano, P. (1993) Induction of apoptosis in cerebellar granule neurons by low potassium: inhibition of death by insulin-like growth factor I and cAMP, Proc. Natl. Acad. Sci. USA, 90: 10989-10993.
3. Dawson, V.L., Dawson, T.M., London, E.D., Bredt, D.S. and Snyder, S.H. (1991) Nitric oxide mediates glutamate neurotoxicity in primary cortical cultures, Proc. Natl. Acad. Sci. USA, 88: 6368-6371.
4. Dessi, F., Charriaut-Marlangue, C., Khrestchatisky, M. and Ben-Ari, Y. (1993) Glutamate-induced neuronal death is not a programmed cell death in cerebellar culture, J. Neurochem., 60: 1953-1955.
5. Drian, M.-J., Kamenka, J.-M., Pirat, J.-L. and Privat, A. (1991) Noncompetitive antagonists of N-methyl-D-aspartate prevent spontaneous neuronal death in primary cultures of embryonic rat cortex, J. Neurosci. Res., 29: 133-138.
6. Frandsen, A. and Schousboe, A. (1990) Development of excitatory amino acid induced cytotoxicity in cultured neurons, Int. J. Dev. Neurosci., 8: 209-216.
7. Johnson, J.E. (1995) Methods for studying cell death and viability in primary neuronal cultures, Methods Cell Biol., 46: 243-276.
8. Koh, J.Y. and Choi, D.W. (1987) Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay, J. Neurosci. Methods, 20: 83-90.
9. Koretz, B., Ahern, K.v.B., Lustig, H.S. and Greenberg, D.A. (1994) Preand postsynaptic modulators of excitatory neurotransmission: comparative effects on hypoxia/hypoglycemia in cortical cultures, Brain Res., 643: 334-337.
10. Lustig, H.S., Chan, J. and Greenberg, D.A. (1992a) Comparative neurotoxic potential of glutamate, endothelins and platelet-activating factor in cerebral cortical cultures, Neurosci. Lett., 139: 15-18.
11. Lustig, H.S., Chan, J. and Greenberg, D.A. (1992b) Ethanol inhibits excitotoxicity in cerebral cortical cultures, Neurosci. Lett., 135: 259-261.
12. Lustig, H.S., Von Brauchitsch, K.L., Chan, J. and Greenberg, D.A. (1992c) Ethanol and excitotoxicity in cultured cortical neurons: Differential sensitivity of N-methyl-D-aspartate and sodium nitroprusside toxicity, J. Neurochem., 59: 2193-2200.
13. Mattson, M.P., Barger, S.W., Begley, J.G. and Mark, R.J. (1995) Calcium, free radicals, and excitotoxic neuronal death in primary cell culture, Methods Cell Biol., 46: 187-216.
14. Pagé, B., Pagé, M. and Noël, C. (1993) A new fluorometric assay for cytotoxicity measurements in vitro, Int. J. Oncol., 3: 473-476.
15. Yan, G.-M., Ni, B., Weller, M., Wood, K.A. and Paul, S.M. (1994) Depolarization or glutamate receptor activation blocks apoptotic cell death of cultured cerebellar granule neurons, Brain Res., 656: 43-51.
16. Yu, A.C.H., Chan, P.H. and Fishman, R.A. (1986) Effects of arachidonic acid on glutamate and γ-aminobutyric acid uptake in primary cultures of rat cerebral cortical astrocytes and neurons, J. Neurochem., 47: 1181-1189.
17. Yu, A.C.H., Chan, P.H. and Fishman, R.A. (1987) Arachidonic acid inhibits uptake of glutamate and glutamine but not of GABA in cultured cerebellar granule cells, J. Neurosci. Res., 17: 424-427.
18. Zhong, J., Russell, S.L., Pritchett, D.B., Molinoff, P.B. and Williams, K. (1994) Expression of mRNAs encoding subunits of the N-methyl-D-aspartate receptor in cultured cortical neurons, Mol. Pharmacol., 45: 846-853.