Construction of luciferase reporter gene vector for human MUC5AC gene promoter and analysis of its transcriptional activity

Journal of Central South University (Medical Sciences)

Volumn 35, Issue 8, 2010, Pages 792-799

  Li, Shengjin ^a   Zhou, Xiangdong ^a  

^a CHONGQING MEDICAL UNIVERSITY   (China)

Author keywords

Gene sequence analysis; Human MUC5AC gene; Promoter; Transcriptional activity analysis

Indexed keywords

IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LUCIFERASE; MUC5AC PROTEIN, HUMAN; MUCIN 5AC;

ARTICLE; DNA SEQUENCE; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HUMAN; LUNG TUMOR; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOLOGY; PROMOTER REGION; REPORTER GENE; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION; TUMOR CELL LINE;

BASE SEQUENCE; CELL LINE, TUMOR; GENES, REPORTER; GENETIC VECTORS; HUMANS; LUCIFERASES; LUNG NEOPLASMS; MOLECULAR SEQUENCE DATA; MUCIN 5AC; MUTAGENESIS, SITE-DIRECTED; NF-KAPPA B; PROMOTER REGIONS, GENETIC; SEQUENCE ANALYSIS, DNA; TRANSCRIPTIONAL ACTIVATION; TRANSFECTION;

EID: 77956697901     PISSN: 16727347     EISSN: None     Source Type: Journal    
DOI: 10.3969/j.issn.1672-7347.2010.08.004     Document Type: Article

References (12)

1. Shao M X , Nadel J A. Neutrophil elastase induces MUC5 AC mucin production in human airway epithelial cells via a cascade involving protein kinase C, reactive oxygen species, and TNF - alpha - converting enzyme [J]. J Immunol, 2005, 175(6) : 4009-4016.
2. Wang B , Lim D J , Han J , et al. Novel cytoplasmic proteins of nontypeable Haemophilus influenzae up-regulate human MUC 5 AC mucin transcription via a positive p38 mitogen-activated protein kinase pathway and a negative phosphoinositide 3-kinase-Akt pathway [J] . J Biol Chem , 2002 , 277 (2) : 949-957. (Pubitemid 34968839)
3. Kim S , Nadel J A. Role of neutrophils in mucus hypersecretion in COPD and implications for therapy [J]. Treat Respir Med, 2004,3(3) : 147-159. (Pubitemid 39004400)
4. Jonckheere N , Van Der Sluis M , Velghe A. et al. Transcriptional activation of the murine Muc5 ac mucin gene in epithelial cancer cells by TGF-β/Smad4 signalling pathway is potentiated by Sp-1 [J]. Biochem J , 2004 , 377 (Pt 3) : 797-808. (Pubitemid 38221854)
5. Li D Z , Gallup M , Fan N , et al. Cloning of the amino - terminal and 5′-flanking region of the human MUC5 AC mucin gene and transcriptional up - regulation by bacterial exoproducts [J]. J Biol Chem, 1998, 273 (12) : 6812-6820. (Pubitemid 28160344)
6. Kato S , Hokari R , Crawley S , et al. MUC5 AC mucin gene regulation in pancreatic cancer cells [J]. Int J Oncol, 2006, 29(1 ) : 33-40.
7. Kadam S , Emerson B M. Mechanisms of chromatin assembly and transcription [J]. Curr Opin Cell Biol, 2002, 14 (3) : 262-268.
8. Boeger H , Bushnell D A , Davis R , et al. Structural basis of eukaryotic gene transcription [J]. FEBS Lett, 2005, 579(4) : 99-903.
9. Romberg R D. The eukaryotic gene transcription machinery [J]. Biol Chem, 2001 ,382 (8): 1103-1107.
10. Schumacher M A , Lau A O, Johnson P J. Structural basis of core promoter recognition in a primitive eukaryote [J]. Cell, 2003, 115(4) : 413-424.
11. Chen Y, Nickola T J , DiFronzo N L, et al. Dexamethasone - mediated repression of MUC 5 AC gene expression in human lung epithelial cells [J]. Am Respir Cell Mol Biol, 2006, 34(3) : 338-347. (Pubitemid 43320715)
12. Hewson C A , Edbrooke M R, Johnston S L. PMA induces the MUC 5 AC respiratory mucin in human bronchial epithelial cells, via PKC, EGF/TGF-alpha, Ras/Raf, MEK, ERK and Sp-I- dependent mechanisms [J]. J Mol Biol, 2004, 344(3) : 683-695.