Primary human osteoblast culture on 3D porous collagen-hydroxyapatite scaffolds

Journal of Biomedical Materials Research - Part A

Volumn 94, Issue 4, 2010, Pages 1244-1250

  Jones, Gemma L ^a   Walton, Robin ^b   Czernuszka, Jan ^b   Griffiths, Sarah L ^a   El Haj, Alicia J ^a   Cartmell, Sarah H ^a  

^a KEELE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Bone tissue engineering; Collagen; Hydroxyapatite; Osteoblasts; Scaffold

Indexed keywords

3D SCAFFOLDS; ALKALINE PHOSPHATASE; ATELOCOLLAGEN; BONE TISSUE ENGINEERING; COLLAGEN MATRICES; DNA ASSAYS; EXTRACELLULAR; EXTRACELLULAR MATRICES; HA POWDERS; MATRIX; MICRO CT; OSTEOBLAST PHENOTYPE; POROUS SCAFFOLD; PRIMARY HUMAN OSTEOBLASTS; SAMPLE VOLUME; VIABILITY STAINING; WEIGHT RATIOS;

ADHESION; APATITE; ASSAYS; BONE; CELL ADHESION; CELL CULTURE; CELL PROLIFERATION; COLLAGEN; COMPUTERIZED TOMOGRAPHY; HYDROXYAPATITE; OSTEOBLASTS; PHOSPHATASES; THREE DIMENSIONAL; TISSUE ENGINEERING;

SCAFFOLDS;

ALKALINE PHOSPHATASE; COLLAGEN; HYDROXYAPATITE; TISSUE SCAFFOLD;

ADULT; ARTICLE; BONE TISSUE; CELL CULTURE; CELL PROLIFERATION; CELL VIABILITY; DNA DETERMINATION; HUMAN; HUMAN CELL; MALE; MICRO-COMPUTED TOMOGRAPHY; OSTEOBLAST; PHENOTYPE; TISSUE ENGINEERING;

ALKALINE PHOSPHATASE; ANIMALS; CATTLE; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COLLAGEN; DNA; DURAPATITE; HUMANS; MALE; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; OSTEOBLASTS; POROSITY; TISSUE SCAFFOLDS; X-RAY MICROTOMOGRAPHY;

EID: 77956483423     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.32805     Document Type: Article

References (28)

1. Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 2005;26:5474-5491.
2. Sachlos E, Reis N, Ainsley C, Derby B, Czernuszka JT. Novel collagen scaffolds with predefined internal morphology made by solid freeform fabrication. Biomaterials 2003;24:1487-1497. (Pubitemid 36069115)
3. Salgado AJ, Coutinho OP, Reis RL. Bone tissue engineering: state of the art and future trends. Macromol Biosci 2004;4:743-765.
4. Sommerfeldt DW, Rubin CT. Biology of bone and how it orchestrates the form and function of the skeleton. Eur Spine J 2001;10(Suppl 2):S86-S95.
5. Buckwalter JA, Glimcher MJ, Cooper RR, Recker R. Bone biology. I: Structure, blood supply, cells, matrix, and mineralization. Instr Course Lect 1996;45:371-386.
6. Alsberg E, Kong HJ, Hirano Y, Smith MK, Albeiruti A, Mooney DJ. Regulating bone formation via controlled scaffold degradation. J Dent Res 2003;82:903-908.
7. Liao S, Wang W, Uo M, Ohkawa S, Akasaka T, Tamura K, Cui F, Watari F. A three-layered nano-carbonated hydroxyapatite/collagen/ PLGA composite membrane for guided tissue regeneration. Biomaterials 2005;26:7564-7571. (Pubitemid 41187556)
8. Middleton JC, Tipton AJ. Synthetic biodegradable polymers as orthopedic devices. Biomaterials 2000;21:2335-2346.
9. Kim SS, Sun Park M, Jeon O, Yong Choi C, Kim BS. Poly(lactideco- glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering. Biomaterials 2005;27:1399-1409.
10. Zhang R, Ma PX. Poly(alpha-hydroxyl acids)/hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology. J Biomed Mater Res 1999;44:446-455.
11. Wahl DA, Czernuszka JT. Collagen-hydroxyapatite composites for hard tissue repair. Eur Cell Mater 2006;11:43-56.
12. Wei G, Ma PX. Structure and properties of nano-hydroxyapatite/ polymer composite scaffolds for bone tissue engineering. Biomaterials 2004;25:4749-4757.
13. Rodrigues CV, Serricella P, Linhares AB, Guerdes RM, Borojevic R, Rossi MA, Duarte ME, Farina M. Characterization of a bovine collagen-hydroxyapatite composite scaffold for bone tissue engineering. Biomaterials 2003;24:4987-4997.
14. Lawson AC, Czernuszka JT. Collagen-calcium phosphate composites. Proc Inst Mech Eng Part H-J Eng Med 1998;212:413-425.
15. Scabbia A, Trombelli L. A comparative study on the use of a HA/ collagen/chondroitin sulphate biomaterial (Biostite) and a bovine-derived HA xenograft (Bio-Oss) in the treatment of deep intra-osseous defects. J Clin Periodontol 2004;31:348-355. (Pubitemid 38624026)
16. Pek YS, Gao S, Arshad MS, Leck KJ, Ying JY. Porous collagen-apatite nanocomposite foams as bone regeneration scaffolds. Biomaterials 2008;29:4300-4305.
17. Anselme K. Osteoblast adhesion on biomaterials. Biomaterials 2000;21:667-681.
18. Bacakova L, Filova E, Rypacek F, Svorcik V, Stary V. Cell adhesion on artificial materials for tissue engineering. Physiol Res 2004;53(Suppl 1):S35-S45.
19. Bender SA, Bumgardner JD, Roach K, Ong JL. Effect of protein on the dissolution of HA coatings. Biomaterials 2000;21:299-305.
20. Boyan BD, Hummert TW, Dean DD, Schwartz Z. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials 1996;17:137-146. (Pubitemid 26031369)
21. Chesmel KD, Black J. Cellular responses to chemical and morphologic aspects of biomaterial surfaces. I. A novel in vitro model system. J Biomed Mater Res 1995;29:1089-1099.
22. Hubbell JA. Biomaterials in tissue engineering. Biotechnol (NY) 1995;13:565-576.
23. Cartmell S, Huynh K, Lin A, Nagaraja S, Guldberg R. Quantitative microcomputed tomography analysis of mineralization within three-dimensional scaffolds in vitro. J Biomed Mater Res A 2004;69:97-104.
24. Schweizer S, Hattendorf B, Schneider P, Aeschlimann B, Gauckler L, Muller R, Gunther D. Preparation and characterization of calibration standards for bone density determination by micro-computed tomography. Analyst 2007;132:1040-1045.
25. Jaecques SV, Van Oosterwyck H, Muraru L, Van Cleynenbreugel T, De Smet E, Wevers M, Naert I, Vander Sloten J. Individualised, micro CT-based finite element modelling as a tool for biomechanical analysis related to tissue engineering of bone. Biomaterials 2004;25:1683-1696. (Pubitemid 38091547)
26. Li WJ, Tuli R, Okafor C, Derfoul A, Danielson KG, Hall DJ, Tuan RS. A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells. Biomaterials 2005;26:599-609. (Pubitemid 38988302)
27. Kikuchi M, Itoh S, Ichinose S, Shinomiya K, Tanaka J. Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo. Biomaterials 2001;22:1705-1711.
28. Rose FR, Cyster LA, Grant DM, Scotchford CA, Howdle SM, Shakesheff KM. In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel. Biomaterials 2004;25:5507-5514.