Efficient silkworm expression of single-chain variable fragment antibody against ginsenoside Re using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its application in enzyme-linked immunosorbent assay for quality control of total ginsenosides

Journal of Biochemistry

Volumn 148, Issue 3, 2010, Pages 335-340

  Sakamoto, Seiichi ^a   Pongkitwitoon, Benyakan ^a   Nakamura, Seiko ^a   Maenaka, Katsumi ^b   Tanaka, Hiroyuki ^a   Morimoto, Satoshi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Bombyx mori nucleopolyhedrovirus; enzyme linked immunosorbent assay; ginsenoside Re; silkworm; single chain variable fragment antibody

Indexed keywords

GINSENOSIDE RE; MELITTIN; SINGLE CHAIN VARIABLE FRAGMENT ANTIBODY; UNCLASSIFIED DRUG; VIRUS ANTIBODY; VIRUS DNA;

ARTHROPOD LARVA; ARTICLE; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; BOMBYX MORI NUCLEAR POLYHEDROSIS VIRUS; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HEMOLYMPH; HONEYBEE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PROTEIN EXPRESSION; QUALITY CONTROL; SILKWORM;

ANIMALS; BACULOVIRIDAE; BOMBYX; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENETIC VECTORS; GINSENOSIDES; NUCLEOPOLYHEDROVIRUS; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES;

APIS MELLIFERA; BOMBYX MORI; BOMBYX MORI NPV; ESCHERICHIA COLI;

EID: 77956428396     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvq072     Document Type: Article

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