Restriction site extension PCR: A novel method for high- throughput characterization of tagged DNA fragments and genome walking

PLoS ONE

Volumn 5, Issue 5, 2010, Pages

  Ji, Jiabing ^a   Braam, Janet ^a  

^a RICE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; ENDONUCLEASE KPNL; ENDONUCLEASE NSIL; ENDONUCLEASE PSTL; ENDONUCLEASE SACL; GENE PRODUCT; GENOMIC DNA; MUTANT PROTEIN; PROTEIN TOUCH4; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARABIDOPSIS; ARTICLE; DNA FLANKING REGION; DNA SEQUENCE; GENE AMPLIFICATION; GENE INSERTION; NONHUMAN; PLANT GENOME; POLYMERASE CHAIN REACTION; RESTRICTION SITE EXTENSION POLYMERASE CHAIN REACTION; TEMPERATURE;

ARABIDOPSIS; BASE SEQUENCE; CHROMOSOME WALKING; DNA RESTRICTION ENZYMES; DNA, BACTERIAL; DNA, INTERGENIC; DNA, PLANT; GENES, PLANT; GENETIC VECTORS; GENOME, PLANT; HIGH-THROUGHPUT SCREENING ASSAYS; MUTAGENESIS, INSERTIONAL; MUTATION; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY; TRANSFORMATION, GENETIC; ZEA MAYS;

ARABIDOPSIS; ZEA MAYS;

EID: 77956412667     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0010577     Document Type: Article

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