Characterization of T-DNA insertion sites in Arabidopsis thaliana and the implications for saturation mutagenesis

OMICS A Journal of Integrative Biology

Volumn 6, Issue 2, 2002, Pages 163-174

  Krysan, Patrick J ^a   Young, Jeffery C ^b   Jester, Peter J ^a   Monson, Sean ^a   Copenhaver, Greg ^c   Preuss, Daphne ^c   Sussman, Michael R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b WESTERN WASHINGTON UNIVERSITY   (United States)

^c UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLANT DNA; TRANSFER RNA;

ARABIDOPSIS; ARTICLE; CHROMOSOME 4; DNA RESPONSIVE ELEMENT; GENE INSERTION; GENETIC TRANSFECTION; KNOCKOUT GENE; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER; TRANSGENIC PLANT;

ARABIDOPSIS; BASE SEQUENCE; CHROMOSOME MAPPING; CHROMOSOMES, PLANT; DNA, BACTERIAL; GENE DELETION; GENES, PLANT; GENOME, PLANT; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PLANTS, GENETICALLY MODIFIED; TRANSFORMATION, GENETIC;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ARABIDOPSIS; ARABIDOPSIS THALIANA; INSERTION SEQUENCES;

EID: 0036407705     PISSN: 15362310     EISSN: None     Source Type: Journal    
DOI: 10.1089/153623102760092760     Document Type: Article

References (11)

1. Copenhaver, G.P., Nickel, K., Kuromori, T., et al. (1999). Genetic definition and sequence analysis of Arabidopsis centromeres. Science 286, 2468-2474.
2. Hajdukiewicz, P., Svab, Z., and Maliga, P. (1994). The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol 25, 989-994.
3. Initiative, T.A.G. (2000). Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796-815.
4. Krysan, P.J., Young, J.C., Tax, F., et al. (1996). Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport. Proc Natl Acad Sci USA 93, 8145-8150.
5. Krysan, P.J., Young, J.C., and Sussman, M.R. (1999). T-DNA as an insertional mutagen in Arabidopsis. Plant Cell 11, 2283-2290.
6. Liu, Y.G., and Whittier, R.F. (1995). Thermal asymmetric interlaced PCR: Automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25, 674-681.
7. Mahalingam, R., and Fedoroff, N. (2001). Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays. Proc Natl Acad Sci USA 98, 7420-7425.
8. Parinov, S., and Sundaresan, V. (2000). Functional genomics in Arabidopsis: Large-scale insertional mutagenesis complements the genome sequencing project. Curr Opin Biotechnol 11, 157-161.
9. Spradling, A.C., Stern, D., Beaton, A., et al. (1999). The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics 153, 135-177.
10. Tissier, A.F., Marillonnet, S., Klimyuk, V., et al. (1999). Multiple independent defective suppressor-mutator transposon insertions in Arabidopsis: A tool for functional genomics. Plant Cell 11, 1841-1852.
11. Walbot, V. (2000). Saturation mutagenesis using maize transposons. Curr Opin Plant Biol 3, 103-107.