Transcriptional regulation of the mexEF-oprN multidrug efflux pump operon by MexT and an unidentified repressor in nfxC-type mutant of Pseudomonas aeruginosa

FEMS Microbiology Letters

Volumn 311, Issue 1, 2010, Pages 36-43

  Maseda, Hideaki ^a   Uwate, Maki ^a   Nakae, Taiji ^b  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b KITASATO INSTITUTE   (Japan)

Author keywords

efflux pump; mexEF oprN; mexT; nod box; Pseudomonas aeruginosa

Indexed keywords

BETA GALACTOSIDASE; NORFLOXACIN; REPRESSOR PROTEIN; RNA POLYMERASE; SPACER DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GROWTH; BACTERIUM MUTANT; BINDING SITE; GEL MOBILITY SHIFT ASSAY; GENE; INVERTED REPEAT; MEXE GENE; MEXEF OPRN OPERON; MEXT GENE; NODULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS AERUGINOSA; REPORTER GENE; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION REGULATION;

BACTERIAL OUTER MEMBRANE PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MUTATION; OPERON; PROTEIN BINDING; PSEUDOMONAS AERUGINOSA; REPRESSOR PROTEINS; TRANSCRIPTION, GENETIC;

PSEUDOMONAS AERUGINOSA;

EID: 77956408650     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02063.x     Document Type: Article

References (21)

1. Fukuda H, Hosaka M, Hirai K Iyobe S (1990) New norfloxacin resistance gene in Pseudomonas aeruginosa PAO. Antimicrob Agents Ch 34 : 1757 1761.
2. Fukuda H, Hosaka M, Iyobe S, Gotoh N, Nishino T Hirai K (1995) nfxC-type quinolone resistance in a clinical isolate of Pseudomonas aeruginosa. Antimicrob Agents Ch 39 : 790 792.
3. Goethals K, Van Montagu M Holsters M (1992) Conserved motifs in a divergent nod box of Azorhizobium caulinodans ORS571 reveal a common structure in promoters regulated by LysR-type proteins. P Natl Acad Sci USA 89 : 1646 1650.
4. Gotoh N, Tsujimoto H, Tsuda M et al 1998) Characterization of the MexC-MexD-OprJ multidrug efflux system in ΔmexA-mexB-oprM mutants of Pseudomonas aeruginosa. Antimicrob Agents Ch 42 : 1938 1943. (Pubitemid 28363073)
5. Köhler T, Michea-Hamzehpour M, Henze U, Gotoh N, Curty LK Pechere JC (1997) Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa. Mol Microbiol 23 : 345 354.
6. Köhler T, Epp SF, Curty LK Pechere JC (1999) Characterization of MexT, the regulator of the MexE-MexF-OprN multidrug efflux system of Pseudomonas aeruginosa. J Bacteriol 181 : 6300 6305.
7. Köhler T, van Delden C, Curty LK, Hamzehpour MM Pechere JC (2001) Overexpression of the MexEF-OprN multidrug efflux system affects cell-to-cell signaling in Pseudomonas aeruginosa. J Bacteriol 183 : 5213 5222.
8. Li XZ, Nikaido H Poole K (1995) Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob Agents Ch 39 : 1948 1953.
9. Maseda H, Saito K, Nakajima A Nakae T (2000) Variation of the mexT gene, a regulator of the MexEF-oprN efflux pump expression in wild-type strains of Pseudomonas aeruginosa. FEMS Microbiol Lett 192 : 107 112.
10. Maseda H, Sawada I, Saito K, Uchiyama H, Nakae T Nomura N (2004) Enhancement of the mexAB-oprM efflux pump expression by a quorum-sensing autoinducer and its cancellation by a regulator, MexT, of the mexEF-oprN efflux pump operon in Pseudomonas aeruginosa. Antimicrob Agents Ch 48 : 1320 1328.
11. Masuda N, Sakagawa E, Ohya S, Gotoh N, Tsujimoto H Nishino T (2000) Contribution of the MexX-MexY-oprM efflux system to intrinsic resistance in Pseudomonas aeruginosa. Antimicrob Agents Ch 44 : 2242 2246.
12. Okazaki T Hirai K (1992) Cloning and nucleotide sequence of the Pseudomonas aeruginosa nfxB gene, conferring resistance to new quinolones. FEMS Microbiol Lett 76 : 197 202.
13. Poole K, Gotoh N, Tsujimoto H et al 1996) Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa. Mol Microbiol 21 : 713 724.
14. Potvin E, Sanschagrin F Levesque RC (2008) Sigma factors in Pseudomonas aeruginosa. FEMS Microbiol Rev 32 : 38 55.
15. Rist M Kertesz MA (1998) Construction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other gram-negative bacteria. FEMS Microbiol Lett 169 : 179 183.
16. Sambrook J, Fritsch EF Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Shiba T, Ishiguro K, Takemoto N, Koibuchi H Sugimoto K (1995) Purification and characterization of the Pseudomonas aeruginosa NfxB protein, the negative regulator of the nfxB gene. J Bacteriol 177 : 5872 5877.
18. Sobel ML, Neshat S Poole K (2005) Mutations in PA2491 (mexS) promote MexT-dependent mexEF-oprN expression and multidrug resistance in a clinical strain of Pseudomonas aeruginosa. J Bacteriol 187 : 1246 1253. (Pubitemid 40299449)
19. Stover CK, Pham XQ, Erwin AL et al 2000) Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature 406 : 959 964.
20. Tian ZX, Fargier E, Mac Aogain M, Adams C, Wang YP O'Gara F (2009) Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa. Nucleic Acids Res 37 : 7546 7559.
21. Yoneyama H, Ocaktan A, Tsuda M Nakae T (1997) The role of mex-gene products in antibiotic extrusion in Pseudomonas aeruginosa. Biochem Bioph Res Co 233 : 611 618.