β-galactosidase assay

Cold Spring Harbor Protocols

Volumn 5, Issue 5, 2010, Pages

  Smale, Stephen T ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

4 NITROPHENYLGALACTOSIDE; BETA GALACTOSIDASE; CHLORAMPHENICOL ACETYLTRANSFERASE; LUCIFERASE; MAGNESIUM CHLORIDE; SODIUM DIHYDROGEN PHOSPHATE;

ARTICLE; BACTERIAL GENE; CELL ADHESION; CELL LYSATE; CENTRIFUGATION; CLINICAL PROTOCOL; COLORIMETRY; CONCENTRATION (PARAMETERS); CYTOLYSIS; ENHANCER REGION; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; EXPERIMENTAL DESIGN; FREEZE THAWING; NONHUMAN; PLASMID; PROCESS DESIGN; PROMOTER REGION; PROTEIN DETERMINATION; QUANTITATIVE ANALYSIS; REPORTER GENE; SPECTROPHOTOMETRY; STABLE TRANSFECTION; TRANSIENT TRANSFECTION; VIRUS GENE;

EID: 77956294977     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5423     Document Type: Article

References (8)

1. Ausubel FM, Brent RE, Kingston E, Moore DD, Seidman JG, Smith JA, Struhl K. 1994. Current protocols in molecular biology. Wiley, New York.
2. Harlow E, Lane D. 2006. Bradford assay. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4644.
3. Smale ST. 2010a. Luciferase assay. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5421.
4. Smale ST. 2010b. Chloramphenicol acetyltransferase assay. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5422.
5. Smale ST. 2010c. Calcium phosphate transfection of 3T3 fibroblasts. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5372.
6. Smale ST. 2010d. DEAE-dextran transfection of lymphocyte cell lines. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5373.
7. Smale ST. 2010e. Transfection by electroporation of RAW 264.7 macrophages. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5374.
8. Young DC, Kingsley SD, Ryan KA, Dutko FJ. 1993. Selective inactivation of eukaryotic β-galactosidase in assays for inhibitors of HIV-1 TAT using bacterial β-galactosidase as a reporter enzyme. Anal Biochem 215: 24-30.