Luciferase assay

Cold Spring Harbor Protocols

Volumn 5, Issue 5, 2010, Pages

  Smale, Stephen T ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; DETERGENT; LUCIFERASE; PLASMID VECTOR;

ACCURACY; ARTICLE; CELL ADHESION; CELL ASSAY; CELL LYSATE; CENTRIFUGATION; CLINICAL PROTOCOL; ENZYME ACTIVITY; ENZYME ASSAY; EXPERIMENTAL DESIGN; FLUORESCENT LIGHTING; FREEZE THAWING; GENETIC TRANSFECTION; PHOTOMETER; PROCESS DESIGN; REPORTER GENE; ROOM TEMPERATURE; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; TRANSIENT TRANSFECTION;

EID: 77956279729     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5421     Document Type: Article

References (9)

1. Ausubel FM, Brent RE, Kingston E, Moore DD, Seidman JG, Smith JA, Struhl K. 1994. Current protocols in molecular biology. Wiley, New York.
2. de Wet JR, Wood KV, DeLuca M, Helinski DR, Subramani S. 1987. Firefly luciferase gene: Structure and expression in mammalian cells. Mol Cell Biol 7: 725-737.
3. Harlow E, Lane D. 2006. Bradford assay. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4644.
4. Nguyen VT, Morange M, Bensaude O. 1988. Firefly luciferase luminescence assays using scintillation counters for quantitation in transfected mammalian cells. Anal Biochem 171: 404-408.
5. Promega Corporation. 1996. Protocols and applications guide, 3rd ed. Promega, Madison, WI.
6. Smale ST. 2010a. Chloramphenicol acetyltransferase assay. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5422.
7. Smale ST. 2010b. Calcium phosphate transfection of 3T3 fibroblasts. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5372.
8. Smale ST. 2010c. DEAE-dextran transfection of lymphocyte cell lines. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5373.
9. Smale ST. 2010d. Transfection by electroporation of RAW 264.7 macrophages. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5374.