G-Less cassette in vitro transcription using HeLa cell nuclear extracts

Cold Spring Harbor Protocols

Volumn 5, Issue 3, 2010, Pages

  Carey, Michael F ^a   Peterson, Craig L ^a   Smale, Stephen T ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT; CIRCULAR DNA; GUANINE; GUANOSINE TRIPHOSPHATE; MESSENGER RNA; PHOSPHORUS 32; RIBONUCLEASE T1; RNA POLYMERASE II; URIDINE TRIPHOSPHATE;

ARTICLE; AUTORADIOGRAPHY; CLINICAL PROTOCOL; DNA SEQUENCE; DNA TEMPLATE; GENE CASSETTE; HELA CELL; IN VITRO STUDY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN FUNCTION; PROTEIN STRUCTURE; RNA ANALYSIS; RNA TRANSCRIPTION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

EID: 77956293370     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5387     Document Type: Article

References (5)

1. II60 of Drosophila. Genes & Dev 11: 3020-3031.
2. Carey MF, Peterson CL, Smale ST. 2009a. Dignam and Roeder nuclear extract preparation. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5330.
3. Carey MF, Peterson CL, Smale ST. 2009b. In vitro transcription using HeLa cell extracts and primer extension. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5331.
4. Sambrook J, Russell DW. 2006. Preparation of denaturing polyacrylamide gels. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3793.
5. Sawadogo M, Roeder RG. 1985. Factors involved in specific transcription by human RNA polymerase II: Analysis by a rapid and quantitative in vitro assay. Proc Natl Acad Sci 82: 4394-4398.