Kinetic resolution of α-methylbenzylamine by recombinant pichia pastoris expressing ω-transaminase

Biotechnology and Bioprocess Engineering

Volumn 15, Issue 3, 2010, Pages 429-434

  Bea, Han Seop ^a   Seo, Young Man ^a   Cha, Min Ho ^b   Kim, Byung Gee ^b   Yun, Hyungdon ^a  

^a Yeungnam University   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

transaminase; Chiral amines; Kinetic resolution; Recombinant Pichia pastoris

Indexed keywords

1-PHENYLETHANOL; ACETOPHENONES; CELL REACTIONS; CHIRAL AMINES; KINETIC RESOLUTION; OXIDOREDUCTASES; P. PASTORIS; PICHIA PASTORIS; PRODUCT INHIBITION; REACTION CONDITIONS; WHOLE CELL BIOCATALYSTS;

AMINES; GLUCOSE; KETONES; METHANOL;

ETHANOL;

1 PHENYLETHANOL; ACETOPHENONE; ALPHA METHYLBENZYLAMINE; AMINOTRANSFERASE; GLUCOSE; HYDROCHLORIC ACID; METHANOL; OMEGA TRANSAMINASE; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYSIS; BIOTECHNOLOGICAL PROCEDURES; CONTROLLED STUDY; FUNGAL METABOLISM; FUNGAL STRAIN; FUNGUS GROWTH; FUNGUS MUTANT; KINETICS; NEGATIVE FEEDBACK; NONHUMAN; PICHIA PASTORIS; PROCESS OPTIMIZATION; REDUCTION;

PICHIA PASTORIS;

EID: 77956215295     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-009-3093-1     Document Type: Article

References (16)

1. Shin, J. -S. and B. -G. Kim (1997) Kinetic resolution of a-methylbenzylamine with ω-transaminase screened from soil microorganisms: Application of a biphasic system to overcome product inhibition. Biotechnol. Bioeng. 55: 348-358.
2. Yun, H., B. -K. Cho, and B. -G. Kim (2004) Kinetic resolution of (R,S)-sec-butylamine using omega-transaminase from Vibrio fluvialis JS17 under reduced pressure. Biotechnol. Bioeng. 87: 772-778.
3. Hwang, B. -Y., B. -K. Cho, H. Yun, K. Koteshwar, and B. -G. Kim (2005) Revisit of aminotransferase in the genomic era and its application to biocatalysis. J. Mol. Cat. B: Enzymatic 37: 47-55.
4. Yun, H. and B. -G. Kim (2008) Asymmetric synthesis of (S)- alpha-methylbenzylamine by recombinant Escherichia coli coexpressing omega-transaminase and acetolactate synthase. Biosci. Biotechnol. Biochem. 72: 3030-3033.
5. Truppo, M. D., N. J. Turner, and J. D. Rozzell (2009) Efficient kinetic resolution of racemic amines using a transaminase in combination with an amino acid oxidase. Chem. Commun. 28: 2127-2129.
6. Höhne, M., S. Kühl, K. Robins, and U. T. Bornscheuer (2008) Efficient asymmetric synthesis of chiral amines by combining transaminase and pyruvate decarboxylase. ChemBioChem 9: 363-365.
7. Iwasaki, A., Y. Yamada, N. Kizaki, Y. Ikenaka, and J. Hasegawa (2006) Microbial synthesis of chiral amines by (R)-specific transamination with Arthrobacter sp. KNK168. Appl. Microbiol. Biotechnol. 69: 499-505.
8. Yun, H., Y. -H. Yang, B. -K. Cho, B. -Y. Hwang, and B. -G. Kim (2003) Simultaneous synthesis of enantiomerically pure (R)-1- phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine using omega-transaminase/alcohol dehydrogenase/glucose dehydrogenase coupling reaction. Biotechnol. Lett. 25: 809-814.
9. Shin, J.-S., H. Yun, J.-W. Jang, I. Park, and B.-G. Kim (2003) Purification, characterization, and molecular cloning of a novel amine: Pyruvate transaminase from Vibrio fluvialis JS17. Appl. Microbiol. Biotechnol. 61: 463-471.
10. Cho, B.-K., J.-H. Seo, J. Kim, C.-S. Lee, and B.-G. Kim (2006) Asymmetric synthesis of unnatural L-amino acids using thermophilic aromatic L-amino acid transaminase. Biotechnol. Bioproc. Eng. 11: 299-305.
11. Koszelewski, D., D. Clay, K. Faber, and W. Kroutil (2009) Synthesis of 4-phenylpyrrolidin-2-one via dynamic kinetic resolution catalyzed by ?-transaminases. J. Mol. Cat. B: Enzymatic 60: 191-194.
12. Schell, U., R. Wohlgemuth, and J. M. Ward (2009) Synthesis of pyridoxamine 5'-phosphate using an MBA: Pyruvate transaminase as biocatalyst. J. Mol. Cat. B: Enzymatic 59: 279-285.
13. Yu, X. -W., L. -L. Wang, and Y. Xu (2009) Rhizopus chinensis lipase: Gene cloning, expression in Pichia pastoris and properties. J. Mol. Cat. B: Enzymatic 57: 304-311.
14. Sambrook, J., E. F. Fritsch, and T. Maniatis (1989) Molecular Cloning: A Laboratory Manual. 2nd ed., pp. 00-00. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
15. Ning, D., X. Junjian, Z. Qing, X. Sheng, C. Wenyin, R. Guirong, and W. Xunzhang (2005) Production of recombinant humanized anti-HBsAg Fab fragment from Pichia pastoris by fermentation. J. Biochem. Mol. Biol. 38: 294-299.
16. Hartner, F. S. and A. Glieder (2006) Regulation of methanol utilization pathway genes in yeasts. Microb. Cell Fact. 5: 39.