Development and preliminary evaluation of a realtime polymerase chain reaction for the identification of Culicoides obsoletus sensu strictu, C. scoticus and C. montanus in the Obsoletus Complex in Italy

Veterinaria Italiana

Volumn 46, Issue 2, 2010, Pages 215-220

  Monaco, Federica ^a   Benedetto, Leonardo ^a   di Marcello, Valeria ^a   Lelli, Rossella ^a   Goffredo, Maria ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL ABRUZZO E DEL MOLISE G CAPORALE   (Italy)

Author keywords

Bluetongue; Culicoides; Culicoides montanus; Culicoides obsoletus; Culicoides scoticus; Italy; Obsoletus complex; PCR; Polymerase chain reaction; Virus

Indexed keywords

CHIRONOMIDAE; CULICOIDES MONTANUS; CULICOIDES OBSOLETUS; CULICOIDES SCOTICUS;

ANIMAL; ARTICLE; CERATOPOGONIDAE; CLASSIFICATION; ITALY; METHODOLOGY; POLYMERASE CHAIN REACTION;

ANIMALS; CERATOPOGONIDAE; ITALY; POLYMERASE CHAIN REACTION;

EID: 77956214252     PISSN: 0505401X     EISSN: 18281427     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Campbell J.A. & Pelham-Clinton E.C. 1960. A taxonomic review of the British species Culicoides Latreille (Diptera, Ceratopogonidae). Proc R Soc Edinb (B), 67, 181-302.
2. Caracappa S., Torina A., Guercio A., Vitale F., Calabrò A., Purpari G., Ferratelli V., Vitale M. & Mellor P.S. 2003. Identification of a novel bluetongue virus vector species of Culicoides in Sicily. Vet Rec, 153, 71-74.
3. Cetre-Sossah C., Baldet T., Delécolle J.-C., Mathieu B., Perrin A., Grillet C. & Albina E. 2004. Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in Africa and Europe. Vet Res, 35, 325-337.
4. Cetre-Sossah C., Mathieu B., Setier-Rio M.L., Grillet C., Baldet T., Delécolle J.-C. & Albina E. 2008. Development and evaluation of a real-time PCR assay for Culicoides imicola, one of the main vectors of bluetongue (BT) and African horse sickness (AHS) in Africa and Europe. Res Vet Sci, 85(2), 372-382.
5. De Liberato C., Scavia G., Lorenzetti R., Scaramozzino P., Amaddeo D., Cardati G., Scicluna M., Ferrari G. & Autorino G.L. 2005. Identification of Culicoides obsoletus (Diptera: Ceratopogonidae) as a vector of bluetongue virus in central Italy. Vet Rec, 156, 301-304.
6. Delécolle J.-C. 1985. Nouvelle contribution à l'étude systématique et iconographique des espèces du genre Culicoides (Diptera: Ceratopogonidae) du nord-est de la France. PhD thesis, Université Louis Pasteur de Strasbourg, UER des sciences de la vie et de la terre, Strasbourg, 238 pp (bluetongue.cirad.fr/FichiersComplementaires/1985_Delecolle_TheseOriginaleCulicoides.pdf accessed on 1 June 2010).
7. de Mattos C.A., de Mattos C.C., Osburn B.I. & Maclachlan N.J. 1994. Evolution of the L2 gene of strains of bluetongue virus serotype 10 isolated in California. Virology, 201, 173-177.
8. Dijkstra E., van der Ven I.J.K., Hölzel D.R., Van Rijn P.A. & Meiswinkel R. 2008. Culicoides chiopterus as a potential vector of bluetongue virus in Europe. Vet Rec, 162, 422.
9. Erasmus B.J. 1990. Bluetongue. In Virus infection of ruminants, Vol. 3 (Z. Dinter & B. Morein, eds). Elsevier Science, Amsterdam, 227-237.
10. Gibbs E.P. & Greiner E.C. 1994. The epidemiology of bluetongue. Comp Immunol Microbiol Infect Dis, 17, 207-220.
11. Goffredo M. & Meiswinkel R. 2004. Entomological surveillance of bluetongue in Italy: methods of capture, catch analysis and identification of Culicoides biting midges. In Bluetongue, Part I. Proceedings Third International Symposium, Taormina, 26-29 October 2003 (N.J. Maclachlan & J.E. Pearson, eds). Vet Ital, 40, 260-265.
12. Goffredo M., Benedetto L., Di Marcello V. & Monaco F. 2008. Identification of C. obsoletus, C. scoticus, C. montanus and C. dewulfi: an integrated morphological and genetic approach. In Proc. Bluetongue Satellite Symposium Bluetongue in Europe: back to the future!, 7 June, Brescia. Epizone, Brescia, 81.
13. Gomulski L.M., Meiswinkel R., Delécolle J.-C., Goffredo M. & Gasperi G. 2005. Phylogenetic relationships of the subgenus Avaritia Fox, 1955 including Culicoides obsoletus (Diptera, Ceratopogonidae) in Italy based on internal transcribed spacer 2 ribosomal DNA sequences. Syst Entomol, 30, 619-631.
14. Italian Ministry of Health 2000. Nota del 6 ottobre 2000 n. 600.6/24461/82N/3903 Sistema Nazionale di Sorveglianza per Bluetongue. Italian Ministry of Health, Rome.
15. Mathieu B., Perrin A., Baldet T., Delécolle J.-C., Albina E. & Cetre-Sossah C. 2007. Molecular identification of Western European species of obsoletus complex (Diptera: Ceratopogonidae) by an internal transcribed spacer-1 rDNA multiplex polymerase chain reaction assay. J Med Entomol, 44(6), 1019-1025.
16. Meiswinkel R., Gomulski L.M., Delécolle J.-C., Goffredo M. & Gasperi G. 2004. The taxonomy of Culicoides vector complexes - unfinished business. In Bluetongue, Part I. Proceedings Third International Symposium, Taormina, 26-29 October 2003 (N.J. Maclachlan & J.E. Pearson, eds). Vet Ital, 40, 151-159.
17. Meiswinkel R., Van Rijn P., Leijs P. & Goffredo M. 2007. Potential new Culicoides vector of bluetongue virus in northern Europe. Vet Rec, 161, 564-565.
18. Nolan D.V., Carpenter S., Barber J., Mellor P.S., Dallas J.F., Mordue A.J. & Piertney S.B. 2007. Rapid diagnostic assays for members of the Culicoides obsoletus and Culicoides pulicaris species complexes, implicated vectors of bluetongue virus in Europe. Vet Microbiol, 124, 82-94.
19. Savini G., Goffredo M., Monaco F., Di Gennaro A., Cafiero M.A., Baldi L., De Santis P., Meiswinkel R. & Caporale V. 2005. Bluetongue virus isolations from midges belonging to the Obsoletus Complex (Culicoides, Diptera: Ceratopogonidae) in Italy. Vet Rec, 157, 133-143.
20. Stephan A., Clausen P.H., Bauer B. & Steuber S. 2009. PCR identification of Culicoides dewulfi midges (Diptera: Ceratopogonidae), potential vectors of bluetongue in Germany. Parasitol Res, 105, 367- 371
21. Thompson J.D., Gibson T.J., Plewiak F., Jeanmougin F. & Higgins D.G. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 25, 4876-4882.