Post-immobilization of modified macromolecular reagents using assembled penicillin acylase for microenvironmental regulation of nanopores and enhancement of enzyme stability

Biotechnology and Bioprocess Engineering

Volumn 15, Issue 3, 2010, Pages 376-382

  Zhou, Cheng ^a   Zhu, Shemin ^a   Wu, Xiuming ^a   Jiang, Bo ^a   Cen, Tao ^a   Shen, Shubao ^a  

^a NANJING TECH UNIVERSITY   (China)

Author keywords

Covalent modification; Immobilized penicillin acylase; Macromolecular crowding; Microenvironment modulation

Indexed keywords

AFTER-TREATMENT; CATALYTIC PERFORMANCE; COMMONLY USED; COVALENT MODIFICATIONS; ENZYME STABILITY; FUNCTIONALIZED; MACROMOLECULAR CROWDING; MICROENVIRONMENTS; OPTIMUM PH; OPTIMUM TEMPERATURE; P-BENZOQUINONE; PENICILLIN ACYLASE; RESIDUAL ACTIVITY; SPECIFIC ACTIVITY; ULTRA-VIOLET;

DEXTRAN; ENZYME IMMOBILIZATION; ENZYMES; GLUCOSE; NANOPORES;

MACROMOLECULES;

1,4 BENZOQUINONE; DEXTRAN; PENICILLIN AMIDASE; REAGENT; SILICATE;

ARTICLE; BACILLUS MEGATERIUM; BIOTECHNOLOGICAL PROCEDURES; CATALYSIS; CHEMICAL MODIFICATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME STABILITY; FOAM; INFRARED SPECTROSCOPY; NANOPORE; NONHUMAN; PROTEIN AGGREGATION; TEMPERATURE;

EID: 77956204999     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-009-0188-7     Document Type: Article

References (17)

1. Chow, Y., R. J. Li, J. C. Wu, S. M. Puah, S. W. New, W. Q. Chia, F. Lie, T. M. M. Rahman, and W. J. Choi (2007) Modeling and optimization of methanol as a cosolvent in amoxicillin synthesis and its advantage over ethylene glycol. Biotechnol. Bioproc. Eng. 12: 390-398.
2. Xue, P., F. Xu, and L. D. Xu (2008) Epoxy-functionalized mesostructured cellular foams as effective support for covalent immobilization of penicillin G acylase. Appl. Surf. Sci. 255: 1625-1630.
3. Chandel, A. K., L. Venkateswar Rao, M. Lakshimi Narasu, and O. V. Singh (2008) The realm of penicillin G acylase in β- lactam antibiotics. Enz. Microb. Technol. 42: 199-207.
4. Minton, A. P. (2001) The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media. J. Biol. Chem. 276: 10577-10580.
5. Cheung, M. S. and D. Thirumalai (2006) Nanopore-protein interactions dramatically alter stability and yield of the native state in restricted spaces. J. Mol. Biol. 357: 632-643.
6. Fernandez-Lafuente, R., C. M. Rosell, L. Caanan-Haden, L. Rodes, and J. M. Guisan (1999) Facile synthesis of artificial enzyme nano-environment via solid-phase chemistry of immobilized derivatives: Dramatic stabilization of penicillin acylase versus organic solvents. Enz. Microb. Technol. 24: 96-103.
7. Wilson, L., A. Illanes, O. Abian, B. C. C. Pessela, R. Fernandez- Lafuente, and J. M. Guisan (2004) Co-aggregation of penicillin G acylase and polyionic polymers: An easy methodology to prepare enzyme biocatalysts stable in organic media. Biomacromolecules 5: 852-857.
8. Zhou, C., A. M. Wang, Z. Q. Du, S. M. Zhu, and S. B. Shen (2009) Improvement of the catalytic performance of immobilized penicillin acylase through assembly of macromolecular reagents in nanpore to create a crowding environment. Korean J. Chem. Eng. 26: 1065-1069.
9. Schmidt-Winkel, P., W. W. Lukens, D. Y. Zhao, P. D. Yang, B. F. Chmelka, and G. D. Stucky (1999) Mesocellular siliceous foams with uniformly sized cells and windows. J. Am. Chem. Soc. 121: 254-255. (Pubitemid 29047147)
10. Wang, A. M., C. Zhou, Z. Q. Du, M. Q. Liu, S. M. Zhu, S. B. Shen, and P. K. Ouyang (2009) Enhancement of microwaveassisted covalent immobilization of penicillin acylase using macromolecular crowding and glycine quenching. J. Biosci. Bioeng. 107: 219-224.
11. Kim, H., J. C. Jung, S. H. Yeom, K. Y. Lee, J. Yi, and I. K. Song (2007) Immobilization of a heteropolyacid catalyst on the aminopropyl-functionalized mesostructured cellular foam (MCF) silica. Mater. Res. Bull. 42: 2132-2142. (Pubitemid 47552484)
12. Van Langen, L. M., M. H. A. Janssen, N. H. P. Oosthoek, S. R. M. Pereira, V. K. Svedas, F. V. Rantwijk, and R. A. Sheldon (2002) Active site titration as a tool for the evaluation of immobilization procedures of penicillin acylase. Biotechnol. Bioeng. 79: 224-228.
13. Shewale, J. G., K. K. Kumar, and G. R. Ambekar (1987) Evaluation of determination of 6-aminopenicillanic acid by pdimethyl aminobenzaldedyde. Biotechnol. Tech. 1: 69-72.
14. Tischer, W. and V. Kasche (1999) Immobilized enzymes: Crystals or carriers?. Trends Biotech. 17: 326-335.
15. Kallenberg, A. I., F. V. Rantwijk, and R. A. Sheldon (2005) Immobilization of penicillin G acylase: The key to optimum performance. Adv. Synth. Catal. 347: 905-926.
16. Chaijitrsakool, T., N. Tonanon, W. Tanthapanichakoon, H. Tamon, and S. Prichanont (2008) Effects of pore characters of mesoporous resorcinol- formaldehyde carbon gels on enzyme immobilization. J. Mol. Catal. B-Enzym. 55: 137-141.
17. Zhang, Z. D., Z. M. He, and M. X. He (2001) Stabilization mechanism of MPEG modified trypsin based on thermal inactivation kinetic analysis and molecular modeling computation. J. Mol. Catal. B-Enzym. 14: 85-94.