In vivo genotoxicity and stress defences in three flatfish species exposed to CuSO4

Ecotoxicology and Environmental Safety

Volumn 73, Issue 6, 2010, Pages 1279-1285

  Chairi, H ^a   Fernandez Diaz C ^b   Navas, J I ^b   Manchado, M ^b   Rebordinos, L ^a   Blasco, J ^c  

^a UNIVERSITY OF CÁDIZ   (Spain)

^b IFAPA CENTRO EL TORUÑO   (Spain)

^c INSTITUTO DE CIENCIAS MARINAS DE ANDALUCÍA   (Spain)

Author keywords

Antioxidant enzymes; Comet assay; CuSO4; Flatfish; Genotoxicity; Stress protein

Indexed keywords

ANTIOXIDANT; CADMIUM; CATALASE; COPPER; COPPER SULFATE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUTATHIONE PEROXIDASE; GLUTATHIONE REDUCTASE; HEAT SHOCK PROTEIN 60; HEAT SHOCK PROTEIN 70; METALLOTHIONEIN; PHOSPHOGLUCONATE DEHYDROGENASE; SUPEROXIDE DISMUTASE; ZINC;

ANTIOXIDANT; CADMIUM; COPPER COMPOUND; ENZYME ACTIVITY; FLATFISH; GENOTOXICITY; METALLOTHIONEIN; PROTEIN; ZINC;

ADULT ANIMAL; ANIMAL EXPERIMENT; ANIMAL HEALTH; ANIMAL TISSUE; ANIMAL WELFARE; ANTIOXIDANT ACTIVITY; AQUACULTURE; ARTICLE; BIOACCUMULATION; COMET ASSAY; CONTENT ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; FLATFISH; GENOTOXICITY; HOST RESISTANCE; IN VIVO STUDY; LIVER LEVEL; NONHUMAN; OXIDATIVE STRESS; SPECIES COMPARISON;

ANIMALS; CATALASE; COMET ASSAY; COPPER SULFATE; DNA DAMAGE; FLATFISHES; HEAT-SHOCK PROTEINS; LIVER; METALLOTHIONEIN; MUTAGENS; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; SPECIES SPECIFICITY; SUPEROXIDE DISMUTASE; WATER POLLUTANTS, CHEMICAL;

DICOLOGLOSSA CUNEATA; PLEURONECTIFORMES; SCOPHTHALMUS RHOMBUS; SOLEA SENEGALENSIS;

EID: 77956191176     PISSN: 01476513     EISSN: 10902414     Source Type: Journal    
DOI: 10.1016/j.ecoenv.2010.07.028     Document Type: Article

References (52)

1. AbouChakra O.R., Joyeux M., Nerriere E., Strub M.P., Zmirou-Navier D. Genotoxicity of organic extracts of urban airborne particulate matter: an assessment within a personal exposure study. Chemosphere 2007, 66:1375-1381.
2. Aebi H. Catalase. Methods of Enzymatic Analysis. 1974, 671-684. Academic Press, London. H.U. Bergmayer (Ed.).
3. Arellano J.M., Storch V., Sarasquete C. Histological changes and copper accumulation in liver and gills of the Senegalese sole, Solea senegalensis. Ecotoxicol. Environ. Saf. 1999, 44:62-72.
4. Arkhipchuk V.V., Garanko N.N. Using the nucleolar biomarker and the micronucleus test on in vivo fish fin cells. Ecotoxicol. Environ. Saf. 2005, 62:42-52.
5. Ay Ö., Kalay M., Tamer L., Canli M. Copper and lead accumulation in tissues of a freshwater fish Tilapia zillii and its effects on the branchial Na, K-ATPase activity. Bull. Environ. Contam. Toxicol. 1999, 62:160-168.
6. Bagdonas E., Vosyliene M.Z. A study of toxicity and genotoxicity of copper, zinc and their mixture, to rainbow trout (Oncorhynchus mykiss). Biologia 2006, 1:8-13.
7. Basu N., Todgham A.E., Ackerman P.A., Bibeau M.R., Nakano K., Schulte P.M., Iwama G.K. Heat shock protein genes and their functional significance in fish. Gene 2002, 295:173-183.
8. Blasco J., Saénz V., Arias A.M., González-Pascual R., Campana O., Ransome T., Moreno-Garrido I., Hampel M. Efecto del accidente minero sobre los organismos residentes en el estuario del Guadalquivir y en las zonas de la plataforma próxima a la desembocadura. Contaminación por metales pesados del estuario del Guadalquivir. Efectos del accidente minero de Aznalcólar sobre el medio físico y los organismos marinos 2003, 165-204. Servicio Publicaciones Universidad de Cádiz. I. Sáenz, J.M. Forja, A. Gómez-Parra (Eds.).
9. Bonamusa P., Garcia de Frutos F., Baanante I.V. Nutritional effects on key glycolytic-gluconeogenic enzyme activities and metabolite levels in the liver of the teleost fish Sparus aurata. Mol. Mar. Biol. Biotechnol. 1992, 1:113-124.
10. Bradford M.B. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
11. Cabrita E., Robles V., Rebordinos L., Sarasquete C., Herráez M.P. Evaluation of DNA damage in rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) cryopreserved sperm. Cryobiology 2005, 50:144-153.
12. Cavas T., Garanko N.N., Arkhipchuk V.V. Induction of micronuclei and binuclei in blood, gill and liver cells of fishes subchronically exposed to cadmium chloride and copper sulphate. Food Chem. Toxicol. 2005, 43:569-574.
13. Cogun H.Y., Yuzereroglu T.A., Kargin F. Accumulation of copper and cadmium in small and large nile tilapia Oreochromis niloticus. Bull. Environ. Contam. Toxicol. 2003, 71:1265-1271.
14. Cohen M.B., Duvel D.L. Characterization of the inhibition of glutathione reductase and the recovery of enzyme activity in exponentially growing murine leukemia (L 1210) cells treated with 1,3-bis(2.chloroethyl-nitrosourea). Biochem. Pharmacol. 1988, 37:3317-3320.
15. Daugaard M., Rohde M., Jaattela M. The heat shock protein 70 family: highly homologous proteins with overlapping and distinct functions. FEBS Lett. 2007, 581:3702-3710.
16. De Boeck G., Meeus W., De Coen W., Blust R. Tissue-specific Cu bioaccumulation patterns and differences in sensitivity to waterborne Cu in three freshwater fish: rainbow trout (Oncorhynchus mykiss), common carp (Cyprinus carpio), and gibel carp (Carassius auratus gibelio). Aquat. Toxicol. 2004, 70:179-188.
17. Fader S.C., Zhongmo Y., Spotila J.R. Seasonal variation in heat shock proteins (HSP70) in stream fish under natural conditions. J. Therm. Biol. 1994, 19:335-341.
18. Feng Q., Boone A.N., Vijayan M.M. Copper impact on heat shock protein 70 expression and apoptosis in rainbow trout hepatocytes. Comp. Biochem. Physiol. 2003, 135:345-355.
19. Flohe L., Gunzler W.A. Assays of glutathione peroxidase. Methods Enzymol. 1984, 105:114-121.
20. Froese, R., Pauly, D., FishBase, 2007. World Wide Web electronic publication. version (01/2007). http://www.fishbase.org.
21. Gabbianelli R., Lupidi G., Villarini M., Falcioni G. DNA damage induced by copper on erythrocytes of Gilthead Sea Bream Sparus aurata and Mollusk Scapharca inaequivalvis. Arch. Environ. Contam. Toxicol. 2003, 45:350-356.
22. Galaris D., Evangelou A. The role of oxidative stress in mechanisms of metal-induced carcinogenesis. Crit. Rev. Oncol. Hematol. 2002, 42:93-103.
23. Garcia O., Romero I., Gonzalez J.E., Mandina T. Measurements of DNA damage on silver stained comets using free Internet software. Mutat. Res. 2007, 627:186-190.
24. George S.G., Todd K., Wright J. Regulation of metallothionein in teleosts: induction of MT mRNA and protein by cadmium in hepatic and extrahepatic tissues of a marine flatfish, the turbot (Scophthalmus maximus). Comp. Biochem. Physiol. 1996, 113(2):109-115.
25. Gravato C., Teles M., Oliveira M., Santos M.A. Oxidative stress, liver biotransformation and genotoxic effects induced by copper in Anguilla anguilla L.-the influence of pre-exposure to b-naphthoflavone. Chemosphere 2006, 65:1821-1830.
26. Helfman G., Collette B., Facey D. The Diversity of Fishes 1997, Blackwell Science, Malden, MA.
27. Jiménez-Cantizano R.M., Infante C., Martin-Antonio B., Ponce M., Hachero I., Navas J.I., Manchado M. Molecular characterization, phylogeny, and expression of c-type and g-type lysozymes in brill (Scophthalmus rhombus). Fish Shellfish Immunol. 2008, 25:57-65.
28. Konca K., Lankoff A., Banasik A., Lisowska H., Kuszewski T., Gozdz S., Koza Z., Wojcik A. A cross-platform public domain PC image-analysis program for the comet assay. Mutat. Res. 2003, 534:15-20.
29. Kopjar N., Garaj-Vrhovac V. Assessment of DNA damage in nuclear medicine personnel-comparative study with the alkaline comet assay and the chromosome aberration test. Int. J. Hyg. Environ. Health 2005, 208:179-191.
30. Lesser M.P. Oxidative stress in marine environments: biochemistry and physiological ecology. Ann. Rev. Physiol. 2006, 68:253-278.
31. Livingstone D.R. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar. Poll. Bull. 2001, 42(8):656-666.
32. Lörh, G., Waller, H., 1974. Methods of Enzymatic Analysis II, pp. 636-643.
33. Luza S.C., Speisky H.C. Liver copper storage and transport during development: implications for cytotoxicity. Am. J. Clin. Nutr. 1996, 63(5):812S-820S.
34. McCord J.M., Fridovich I. Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 1969, 244:6049-6055.
35. McFarland V.A., Inouye S., Lutz C.H., Jarvis A.S., Clarke J.U., McCant D.D. Biomarkers of oxidative stress and genotoxicity in livers of field collected brown bullhead, Ameirus nebulosus. Arch. Environ. Contam. Toxicol. 1999, 37:236-241.
36. Martín-Díaz M.L., Blasco J., González de Canales M.L., Sales D., DelValls T.A. Bioaccumulation and toxicity of dissolved heavy metals from the Guadalquivir Estuary after the Aznalcollar mining spill using Ruditapes philippinarum. Arch. Environ. Contam. Toxicol. 2005, 48:233-241.
37. Monteiro S.M., Rocha E., Mancera J.M., Fontaínhas-Fernandes A., Sousa M. A stereological study of copper toxicity in gills of Oreochromis niloticus. Ecotoxicol. Environ. Saf. 2009, 72:213-223.
38. Neri M., Ugolini D., Bonassi S., Fucic A., Holland N., Knudsen L.E., Sram R.J., Ceppi M., Bocchini V., Merlo D.F. Children's exposure to environmental pollutants and biomarkers of genetic damage-II. Results of a comprehensive literature search and meta-analysis. Mutat. Res. 2006, 612:14-39.
39. Nogueira P.R., Lourenc J., Mendo S., Rotchell J.M. Mutation analysis of ras gene in the liver of European eel (Anguilla anguilla L.) exposed to benzo[a]pyrene. Mar. Poll. Bull. 2006, 52:1611-1616.
40. Olafson R.W., Olsson P.E. Electrochemical detection of metallothionein, zinc and copper levels during an annual reproductive cycle in Rainbow trout (Salmo gairdneri). Fish Physiol. Biochem. 1987, 31:39-47.
41. Oliva M., Garrido M.C., Pérez E., González de Canales M.L. Evaluation of acute copper toxicity during early life stages of gilthead seabream, Sparus aurata. J. Environ. Sci. Health A: Tox. Hazard Subst. Environ. Eng. 2007, 42:525-533.
42. Oliva M., Garrido M.C., Marquez D.S., González de Canales M.L. Sublethal and lethal toxicity in juvenile Senegal sole (Solea senegalensis) exposed to copper: a preliminary toxicity range-finding test. Exp. Toxicol. Pathol. 2009, 61:113-121.
43. Oliveira M., Serafim A., Bebianno M.J., Pacheco M., Santos M.A. European eel (Anguilla anguilla L.) metallothionein, endocrine, metabolic and genotoxic responses to copper exposure. Ecotoxicol. Environ. Saf. 2008, 70:20-26.
44. Pruski A.M., Dixon D.R. Heat shock protein expression pattern (HSP70) in the hydrothermal vent mussel Bathymodiolus azoricus. Mar. Environ. Res. 2007, 64:209-224.
45. Rainbow P.S. Heavy metals in marine invertebrates. Heavy Metals in the Marine Environment 1990, 67-80. CRC Press, Boca Raton, FL. R.W. Furness, P.S. Rainbow (Eds.).
46. Roméo M., Cosson R.P., Gnassia-Barelli M., Risso C., Stien X., Lafaurie M. Metallothionein determination in the liver of the sea bass Dicentrarchus labrax treated with copper and B(a)P. Mar. Environ. Res. 1997, 44(3):275-284.
47. Roméo M., Bennani N., Gnasia-Barelli M., Lafaurie M., Girard J.P. Cadmium and copper display different responses towards oxidative stress in the kidney of the sea bass Dicentrachus labrax. Aquat. Toxicol. 2000, 48:185-194.
48. Solé M., Potrykus J., Fernández-Diaz C., Blasco J. Variations on stress defences and metallothionein levels in the Senegal sole, Solea senegalensis, during early larval stages. Fish Physiol. Biochem. 2004, 30:57-66.
49. Tice R.R. The single cell gel/comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells. Environmental Mutagenesis 1996, 315-339. BIOS Scientific Publishers, Oxford, UK. D.H. Phillips, S. Venitt (Eds.).
50. Viarengo A., Burlando B., Cavaletto M., Marchi B., Ponzano E., Blasco J. Role of metallothionein against oxidative stress in the mussel (Mytilus galloprovincialis). Am. J. Physiol. Regul. Integr. Comp. Physiol. 1999, 277:1612-1619.
51. Widianarko B., Van Gestel C.A.M., Verweij R.A., Van Straalen N.M. Associations between trace metals in sediment, water, and guppy, Poecilia reticulata (Peters), from urban streams of Semarang, Indonesia. Ecotoxicol. Environ. Saf. 2000, 46:101-107.
52. Zhou J.L., Salvador S.M., Liu Y.P., Sequeira M. Heavy metals in the tissues of common dolphins (Delphinus delphis) stranded on the Portuguese coast. Sci. Total Environ. 2001, 273:61-76.