Genetic detection and quantification of Nosema apis and N. ceranae in the honey bee

Journal of Invertebrate Pathology

Volumn 103, Issue 1, 2010, Pages 53-58

  Bourgeois, A Lelania ^a   Rinderer, Thomas E ^a   Beaman, Lorraine D ^a   Danka, Robert G ^a  

^a U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

Genetic detection; Nosema apis; Nosema ceranae; Real time PCR

Indexed keywords

FUNGAL DNA;

ANIMAL; ARTICLE; BEE; FUNGUS SPORE; GENETICS; INCIDENCE; ISOLATION AND PURIFICATION; MICROBIOLOGY; MICROSPORIDIOSIS; NOSEMA; SEASON;

ANIMALS; BEES; DNA, FUNGAL; INCIDENCE; MICROSPORIDIOSIS; NOSEMA; SEASONS; SPORES, FUNGAL;

APIS MELLIFERA; APOIDEA; BACTERIA (MICROORGANISMS); NOSEMA; NOSEMA APIS; NOSEMA CERANAE;

EID: 72049130798     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2009.10.009     Document Type: Article

References (24)

1. Altschul S.F., Madden T.L., Schiñffer A.A., Zhang J., Zhang Z., Miller W., and Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25 (1997) 3389-3402
2. Bailey, L., 1981. Honey bee pathology. Honey Bee Pathology.
3. Bilodeau A.L., Waldbieser G.C., Wolters W.R., Terhune J.S., and Wise D.J. A real-time polymerase chain reaction assay of the bacterium Edwardsiella ictaluri in channel catfish. Journal of Aquatic Animal Health 15 (2003) 80-86
4. Chauzat M.P., Higes M., Martin-Hernandez R., Meana A., Cougoule N., and Faucon J.P. Presence of Nosema ceranae in French honey bee colonies. Journal of Apicultural Research 46 (2007) 127-128
5. Chen Y., Evans J.D., Smith I.B., and Pettis J.S. Nosema ceranae is a long-present and wide-spread microsporidian infection of the European honey bee (Apis mellifera) in the United States. Journal of Invertebrate Pathology 97 (2008) 186-188
6. Fries I., Ekbohm G., and Villumstad E. Nosema apis, sampling techniques and honey yield. Journal of Apicultural Research 23 (1984) 102-105
7. Fries I., Feng F., Da Silva A., Slemenda S.B., and Pieniazek N.J. Nosema ceranae n. sp. (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honey bee Apis cerana (Hymenoptera, Apidae). European Journal of Protistology 32 (1996) 356-365
8. Fries I., Martin-Hernandez R., Meana A., Palencia P., and Higes M. Natural infections of Nosema ceranae in European honey bees. Journal of Apicultural Research 45 (2006) 230-233
9. Hall T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Research 41 (1999) 95-98
10. Higes M., Martin-Hernandez R., Botias C., Bailon E.G., Gonzalez-Porto A.V., Barrios L., Del Nozal M.J., Bernal J.L., Jimenez J.J., Palencia P.G., and Meana A. How natural infection by Nosema ceranae causes honeybee colony collapse. Environmental Microbiology 10 (2008) 2659-2669
11. Higes M., Martin-Hernandez R., Garrido B., Palencia P., and Meana A. Detection of infective Nosema ceranae (Microsporidia) spores in corbicular pollen of forager honeybees. Journal of Invertebrate Pathology 97 (2008) 76-78
12. Higes M., Martin-Hernandez R., and Meana A. Nosema ceranae, a new microsporidian parasite in honeybees in Europe. Journal of Invertebrate Pathology 92 (2006) 81-83
13. Higes M., Palencia P., Martin-Hernandez R., and Meana A. Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia). Journal of Invertebrate Pathology 94 (2007) 211-217
14. Huang W.F., Jiang J.H., Chen Y.W., and Wang C.H. A Nosema ceranae isolate from the honeybee Apis mellifera. Apidologie 38 (2007) 30-37
15. Kurkela S., and Brown D.W.G. Molecular diagnostic techniques. Medicine 37 (2009) 535-540
16. Malone L.A., Giacon H.A., and Newton M.R. Comparison of the responses of some New Zealand and Australian honey bees (Apis mellifera L.) to Nosema apis Z. Apidologie 26 (1995) 495-502
17. Malone L.A., and Stefanovic D. Comparison of the response of two races of honeybees to infection with Nosema apis Zander. Apidologie 30 (1999) 375-382
18. Martin-Hernandez R., Meana A., Prieto L., Salvador A.M., Garrido B., and Higes M. Outcome of colonization of Apis mellifera by Nosema ceranae. Applied and Environmental Microbiology 73 (2007) 6331-6338
19. Matheson A. World bee health report. Bee World 74 (1993) 176-212
20. Paxton R.J., Klee J., Korpela S., and Fries I. Nosema ceranae has infected Apis mellifera in Europe since at least 1998 and may be more virulent than Nosema apis. Apidologie 38 (2007) 558-565
21. Rinderer T.E., and Sylvester H.A. Variation in response to Nosema apis, longevity, and hoarding behavior in a free-mating population of the honey bee. Annals of the Entomological Society of America 71 (1978) 372-374
22. Topolska G., and Kasprzak S. First cases of Nosema ceranae infection in bees in Poland. Medycyna Weterynaryjna 63 (2007) 1504-1506
23. Williams G.R., Shafer A.B.A., Rogers R.E.L., Shutler D., and Stewart D.T. First detection of Nosema ceranae, a microsporidian parasite of European honey bees (Apis mellifera), in Canada and central USA. Journal of Invertebrate Pathology 97 (2008) 189-192
24. Yan D., Tang K.F.J., and Lightner D.V. Development of a real-time PCR assay for detection of monodon baculovirus (MBV) in penaeid shrimp. Journal of Invertebrate Pathology 102 (2009) 97-100