Gene cloning and expression of a detergent stable alkaline protease from Aspergillus clavatus ES1

Process Biochemistry

Volumn 45, Issue 10, 2010, Pages 1746-1752

  Hajji, Mohamed ^a   Hmidet, Noomen ^a   Jellouli, Kemel ^a   Vallaeys, Tatiana ^b   Nasri, Moncef ^a   Sellami Kamoun, Alya ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

^b UNIV MONTPELLIER   (France)

Author keywords

Alkaline protease; Aspergillus clavatus ES1; Detergent; Expression; Gene cloning

Indexed keywords

ALKALINE PROTEASE; AMINO ACID RESIDUES; ASPERGILLUS CLAVATUS ES1; CATALYTIC EFFICIENCIES; EXPRESSION; GENE CLONING; GENE CLONING AND EXPRESSIONS; GENOMIC DNA; MATURE PROTEINS; OPEN READING FRAME; SDS-PAGE; SEQUENCE ANALYSIS; SIGNAL PEPTIDE;

AMINO ACIDS; CLONING; DETERGENTS; DNA; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR WEIGHT; ORGANIC ACIDS; PEPTIDES;

GENE ENCODING;

ASPERGILLUS; ASPERGILLUS CLAVATUS; ESCHERICHIA COLI;

EID: 77956171445     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.07.005     Document Type: Article

References (29)

1. Handel RA, Rhode J, Rodger R. Proteolytic fungal enzyme food supplement composition. Patent 5387422 (February 7, 1995).
2. Rao M.B., Tanksale A.M., Ghatge M.S., Deshpande V.V. Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 1998, 62:597-635.
3. Kumar C.G., Takagi H. Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnol Adv 1999, 17:561-594.
4. Horikoshi K. Alkalophiles: from an industrial point of view. FEMS Microbiol 1996, 18:259-270.
5. Maurer K.H. Detergent proteases. Curr Opin Biotechnol 2004, 15:330-334.
6. Mitchell D.A., Lonsane B.K. Definition, characteristics and potential in solid state cultivation. Applied biotechnology series 1992, 1-16. Elsevier, Amsterdam. H.W. Doelle, S.A. Mitchell, C.E. Rolz (Eds.).
7. Phadatare S.U., Deshpande V.V., Srinivasan M.C. High activity alkaline protease from Conidiobolus coronatus (NCL 86.8.20): enzyme production and compatibility with commercial detergents. Enzyme Microb Technol 1993, 15:72-76.
8. Tunlid A., Rosen S., Ek B., Rask L. Purification and characterization of an extracellular serine protease from the nematode-trapping fungus Arthrobotrys oligospora. Microbiology 1994, 140:1687-1695.
9. Dunaevsky T.E., Gruban T.N., Beliokova G.A., Belozersky M.A. Enzymes secreted by filamentous fungi, regulation and purification of an extracellular protease of Trichoderma harzianum. Biochemistry (Moscow) 2000, 65:723-727.
10. Tsuchiya K., Arai T., Seki K., Kimura T. Purification and some properties of alkaline proteinases from Cephalosporium sp. KM388. Agric Biol Chem 1987, 51:2959-2965.
11. Wang S.L., Chen Y.H., Wang C.L., Yen Y.H., Chern M.K. Purification and characterization of a serine protease extracellularly produced by Aspergillus fumigatus in a shrimp and crab shell powder medium. Enzyme Microb Technol 2005, 36:660-665.
12. Laxman R.S., Sonawane A.P., More S.V., Rao B.S., Rele M.V., Jogdand V.V., et al. Optimization and scale up of production of alkaline protease from Conidiobolus coronatus. Process Biochem 2005, 40:3152-3158.
13. Gupta R., Beg Q.K., Lorenz P. Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol 2002, 59:15-32.
14. Outtrup H, Dambmann C, Christiansen M, Aaslyng D. Bacillus sp. JP 395, method of making and detergent composition. United States Patent Number 5466594 (1995).
15. Berka R.M., Ward M., Wilson L.J., Hayenga K.J., Kodama K.H., Carlomango L.P., et al. Molecular cloning and deletion of the gene encoding aspergillopepsin A from Aspergillus awamori. Gene 1990, 86:153-162.
16. Li J., Chi Z., Wang X. Cloning of SAP6 gene of Metschnikowia reukaufii and its heterologous expression and characterization in Escherichia coli. Microbiol Res 2010, 165:173-182.
17. Guo J.P., Ma Y. High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris. Protein Exp Purif 2008, 58:301-308.
18. Katia J.O., Mark S., Reto C., Rocco F., Abdelouahad F., Michel M. Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease from Aspergillus fumigatus. FEMS Microbiol Lett 1992, 92:163-168.
19. Hajji M., Kanoun S., Nasri M., Gharsallah N. Purification and characterization of an alkaline serine-protease produced by a new isolated Aspergillus clavatus ES1. Process Biochem 2007, 42:791-797.
20. Sambrook J., Fritsch E.F., Maniatis T. A laboratory manual 1989, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 2nd edition.
21. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J Mol Biol 1990, 215:403-410.
22. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
23. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
24. Kembhavi A.A., Kulkarni A., Pant A. Salt-tolerant and thermostable alkaline protease from Bacillus subtilis NCIM No. 64. Appl Biochem Biotechnol 1993, 38:83-92.
25. Lineweaver H., Burk D. The determination of enzyme dissociation constant. J Am Chem Sci 1934, 56:665-666.
26. Katz M.E., Rice R.N., Cheetham B.F. Isolation and characterization of an Aspergillus nidulans gene encoding alkaline protease. Gene 1994, 150:287-292.
27. Ni X., Chi Z., Ma C.L., Madzak C. Cloning, characterization and expression of the gene encoding alkaline protease in the marine yeast Aureobasidium pullulans 10. Mar Biotechnol 2008, 10:319-327.
28. Ni X., Yue L., Chi Z., Li J., Wang X., Madzak C. Alkaline protease gene cloning from the marine yeast Aureobasidium pullulans HN2-3 and the protease surface display on Yarrowia lipolytica for bioactive peptide production. Mar Biotechnol 2009, 11:81-89.
29. Galagan J.E., Calvo S.E., Cuomo C., Ma L.J., Wortman J.R., Batzoglou S., et al. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 2005, 438:1105-1115.