Purification and characterization of a serine protease extracellularly produced by Aspergillus fumigatus in a shrimp and crab shell powder medium

Enzyme and Microbial Technology

Volumn 36, Issue 5-6, 2005, Pages 660-665

  Wang, San Lang ^a,d   Chen, Yen Hsu ^b   Wang, Chuan Lu ^c   Yen, Yue Horng ^b   Chern, Ming Kai ^a  

^a TAMKANG UNIVERSITY   (Taiwan)

^b DA YEH UNIVERSITY   (Taiwan)

^c LAN YANG INSTITUTE OF TECHNOLOGY   (Taiwan)

^d NONE   (Taiwan)

Author keywords

Aspergillus fumigatus; Chitinase; Protease; Shrimp and crab shell

Indexed keywords

BIODIVERSITY; ELECTROPHORESIS; FUNGI; GROWTH KINETICS; MARINE APPLICATIONS; MOLECULAR WEIGHT; PH EFFECTS; PURIFICATION; SOILS; THERMODYNAMIC STABILITY;

MARINE WASTE; PROTEASE; SERINE PROTEASE; SHRIMP AND CRAB SHELL POWDER (SCSP);

ENZYMES;

CHITINASE; FUNGAL ENZYME; PROTEINASE; SERINE PROTEINASE;

ARTICLE; ASPERGILLUS FUMIGATUS; CRAB; ENZYME ACTIVITY; ENZYME INHIBITION; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS ISOLATION; MARINE ENVIRONMENT; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SHRIMP; SUPERNATANT; TEMPERATURE; THERMOSTABILITY; WASTE;

ASPERGILLUS FUMIGATUS; DECAPODA (CRUSTACEA); FUNGI;

EID: 14544270524     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2004.10.006     Document Type: Article

References (20)

1. G.P. Bodey, and S. Vartivarian Aspergillosis Eur J Clin Microbiol Infect Dis 8 1989 413 437
2. U. Reichard, M. Monod, and R. Ruchel Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from Aspergillus fumigatus FEMS Microbiol Lett 130 1995 69 74
3. A.G. Jonsson, and S.M. Martin Protease production by Aspergillus fumigatus Agric Biol Chem 28 1964 734 739
4. U. Reichard, S. Buttner, H. Eiffert, F. Staib, and R. Ruchel Purification and characterization of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue J Med Microbiol 33 1990 243 251
5. Y.S. Oh, I.L. Shih, Y.M. Tzeng, and S.L. Wang Protease produced by Pseudomonas aeruginosa K-187 and its application in the deproteinization of shrimp and crab shell wastes Enzyme Microb Technol 27 2000 3 10
6. S.L. Wang, and W.T. Chang Purification and characterization of two bifunctional chitinases/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium Appl Environ Microbiol 63 1997 380 386
7. U. Reichard, S. Buttner, H. Eiffert, F. Staib, and R. Ruchel Purification and characterization of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue J Med Microbiol 33 1990 243 251
8. G. Larcher, J.P. Bouchara, V. Annaix, F. Symoens, F. Chabasse, and G. Tronchin Purification and characterization of a fibrinogenolytic serine proteinase from Aspergillus fumigatus culture filtrate FEBS Lett 308 1992 65 69
9. M. Monod, G. Togni, L. Rahalison, and E. Frenk Isolation and characterization of an extracellular alkaline protease of Aspergillus fumigatus J Med Microbiol 35 1991 23 28
10. U. Reichard, M. Monod, and R. Ruchel Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from Aspergillus fumigatus FEMS Microbiol Lett 130 1995 69 74
11. U. Reichard, H. Eiffert, and R. Ruchel Purification and characterization of an extracellular aspartic proteinase from Aspergillus fumigatus J Med Vet Mycol 32 1994 427 436
12. M. Monod, S. Paris, D. Sanglard, K. Jaton-Ogay, J. Bille, and J.P. Latge Isolation and characterization of a secreted metalloprotease of Aspergillus fumigatus Infect Immun 61 1993 4099 4104
13. A. Markaryan, I. Morozova, H. Yu, and P.E. Kolattukudy Purification and characterization of an elastinolytic metalloprotease from Aspergillus fumigatus and immunoelectron microscopic evidence of secretion of this enzyme by the fungus invading the murine lung Infect Immun 62 1994 2149 2157
14. C. Jeniaux Chitinase Methods Enzymol 8 1966 644 650
15. E.W. Todd Quantitative studies on the total plasmin and trypsin inhibitor of human blood serum J Exp Med 39 1949 295 308
16. C. Jeniaux Recherches sur les chitinases. I. Dosage nephelometrique et production de chitinase par des Streptomyces Arch Int Physiol Biochem 66 1958 408 427
17. T. Imoto, and K. Yagishita A simple activity measurement by lysozyme Agric Biol Chem 35 1971 1154 1156
18. K. Weber, and M. Osborn The reliability of molecular weight determination by dodesylsulfate-polyacrylamide gel electrophoresis J Biol Chem 244 1969 4406 4412
19. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
20. A. Beauvais, M. Monod, J.P. Debeaupuis, M. Diaquin, H. Kobayashi, and J.P. Latge Biochemical and antigenic characterization of a new dipeptidyl-peptidase isolated from Aspergillus fumigatus J Biol Chem 272 1997 6238 6244