Quantification of the rice false smut pathogen Ustilaginoidea virens from soil in Japan using real-time PCR

European Journal of Plant Pathology

Volumn 128, Issue 2, 2010, Pages 221-232

  Ashizawa, Taketo ^a   Takahashi, Mami ^a   Moriwaki, Jouji ^b   Hirayae, Kazuyuki ^c  

^a FOOD RESEARCH INSTITUTE   (Japan)

^b Toyama Agricultural Research Center   (Japan)

^c NATIONAL AGRICULTURAL RESEARCH CENTER FOR KYUSHU OKINAWA REGION   (Japan)

Author keywords

LightCycler; Villosiclava

Indexed keywords

ANDOSOL; CORRELATION; DISEASE CONTROL; FUNGAL DISEASE; FUNGUS; GLEYSOL; LINEARITY; MITOCHONDRIAL DNA; OPTIMIZATION; PADDY FIELD; PATHOGEN; POLYMERASE CHAIN REACTION; REAL TIME; RICE; SOIL MICROORGANISM;

JAPAN;

BACTERIA (MICROORGANISMS); FUNGI; USTILAGINOIDEA VIRENS;

EID: 77956170239     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10658-010-9647-4     Document Type: Article

References (20)

1. Ashizawa, T., & Kataoka, Y. (2005). Detection of Ustilaginoidea virens in rice panicles before and after heading in the field using nested-PCR technique with species-specific primers. Japanese Journal of Phytopathology, 71, 16-19.
2. Bischoff, J. F., Sullivan, R. F., Kjer, K. M., & White, J. F., Jr. (2004). Phylogenetic placement of the anamorphic tribe Ustilaginoideae (Hypocreales, Ascomycota). Mycologia, 96, 1088-1094.
3. Brooks, S. A., Anders, M. M., & Yeater, K. M. (2009). Effect of cultural management practices on the severity of false smut and kernel smut of rice. Plant Disease, 93, 1202-1208. doi: 10. 1094/PDIS-93-11-1202.
4. Cullen, D. W., Lees, A. K., Toth, I. K., & Duncan, J. M. (2001). Conventional PCR and real-time quantitative PCR detection of Helminthosporium solani in soil and on potato tubers. European Journal of Plant Pathology, 107, 387-398.
5. Cullen, D. W., Lees, A. K., Toth, I. K., & Duncan, J. M. (2002). Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR. Plant Pathology, 51, 281-292.
6. Deng, G. S. (1989). Present status of research on false smut in China. Plant Protection Beijing, 15, 39-40.
7. Ikegami, H. (1963). Invasion of chlamydospores and hyphae of the false smut fungus into rice plants. Research Bulletin of the Faculty of Agriculture, Gifu University, 18, 54-60.
8. Kageyama, K., Komatsu, T., & Suga, H. (2003). Refined PCR protocol for detection of plant pathogens in soil. Journal of General Plant Pathology, 69, 153-160. doi: 10. 1007/s10327-002-0037-4.
9. Okubara, P. A., Schroeder, K. L., & Paulitz, T. C. (2005). Real-time polymerase chain reaction: applications to studies on soilborne pathogens. Canadian Journal of Plant Pathology, 27, 300-313.
10. Osada, S. (1995). Effect of false smut occurrence on yield and quality of rice. Annual Report of the Society of Plant Protection of North Japan, 46, 30-32.
11. Ou, S. H. (1972). Rice diseases. Kew: Commonwealth Mycological Institute.
12. Takahashi, Y. (1896). On Ustilago virens Cooke and a New Species of Tilletia parasitic on rice plant. Botanical Magagine, Tokyo, 10, 16-20.
13. Tanaka, E., & Tanaka, C. (2008). Phylogenetic study of clavicipitaceous fungi using acetaldehyde dehydrogenase gene sequences. Mycoscience, 49, 115-125. doi: 10. 1007/s10267-007-0401-5.
14. Tanaka, T., Ashizawa, T., Sonoda, R., & Tanaka, C. (2008). Villosiclava virens gen. nov., com. nov., teleomorph of Ustilaginoidea virens, the causal agent of rice false smut. Mycotaxon, 106, 491-501.
15. Tsuda, M., Sasahara, M., Ohara, T., & Kato, S. (2006). Optimal application timing of simeconazole granules for control of rice kernel smut and false smut. Journal of General Plant Patholology, 72, 301-304. doi: 10. 1007/s10327-006-0288-6.
16. Volossiouk, T., Robb, E. J., & Nazar, R. N. (1995). Direct DNA extraction for PCR-mediated assays of soil organisms. Applied and Environmental Microbiology, 61, 3972-3976.
17. White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315-322). California: San Diego.
18. White, J. F., Jr., Sullivan, R., Moy, M., Patel, R., & Duncan, R. (1990). An overview of some current problems in the classification of plant-parasitic Clavicipitaceae. Studies in Mycology, 45, 95-105.
19. Yaegashi, H., Fujita, Y., & Sonoda, R. (1989). Severe outbreak of false smut of rice in 1988. Plant Protection Tokyo, 43, 311-314.
20. Zhou, Y.-L., Izumitsu, K., Sonoda, R., Nakazaki, T., Tanaka, E., Tsuda, M., et al. (2003). PCR-based specific detection of Ustilaginoidea virens and Ephelis japonica. Journal of Phytopathology, 151, 513-518.