NOR1 enhaced 5516376in HepG2 is dependent on Grb2 mediated MAPK pathway

2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010

Volumn , Issue , 2010, Pages

  Gui, Rong ^a   Liu, Jing ^a   Li, Dengqing ^b   Tang, Hua ^b   Feng, Zhenqian ^c   Nie, Xinmin ^a,c  

^a THE THIRD XIANGYA HOSPITAL OF CENTRAL SOUTH UNIVERSITY   (China)

^b Central South University   (China)

^c MCGILL UNIVERSITY   (Canada)

Author keywords

Grb2; MAPK; Nor1; ShRNA

Indexed keywords

CANCER CELLS; CELL KILLING; CELL LINES; GENESTEIN; GRB2; HEPG2 CELLS; MAPK; NASOPHARYNGEAL CARCINOMA; NITRO GROUP; NITROREDUCTASES; NOR1; OVER-EXPRESSION; PROTEIN EXPRESSIONS; QUANTITATIVE REAL TIME PCR; SHRNA; TYROSINE KINASE INHIBITOR; WESTERN-BLOT ANALYSIS;

AMINO ACIDS; BIOINFORMATICS; CELL CULTURE; NUCLEIC ACIDS; PHOSPHATASES; SIGNAL TRANSDUCTION; TOXICITY;

GENE EXPRESSION;

EID: 77956150832     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/ICBBE.2010.5516376     Document Type: Conference Paper

References (22)

1. Zhang BC, Nie XM, Xiao BY, et al. Identification of tissue -specific genes in nasopharyngeal epithelial tissue and differentially expressed genes in nasopharyngeal carcinoma by suppression subtractive hybridization and cDNA microarray. Genes, chromosomes & Cancer. 2003,38(1):80-90.
2. Nie XM ,Zhang BC, Li XL, et al. Cloning, Expression and Mutation analysis of NOR1, a novel human gene down-regulated in HNE1 nasopharyngeal carcinoma cell line. J Cancer Res Clin Oncol. 2003,129 (7) : 410-414.
3. Nie XM, Zhou M,Tang K, et al. Molecular Cloning and Characterization of a Novel NitroreductaseGene.NOR1, Possibly Involved in Chemical Carcinogenesis of NPC.Chin J Biochemistry and Molecular Biology. 2003,19(4):423-428.
4. Poirier S, Bouvier G, Malaveille C, et al. Volatile nitrosamine levels and genotoxicity of food samples from high-risk areas for nasopharyngeal carcinoma before and after nirosation.Int J Cancer, 1989,44(6): 1088-1094.
5. Wang Y, Ausman LM, Greenberg AS.et al. Nonalcoholic steatohepatitis induced by a high-fat diet promotes diethylnitrosamine-initiate early hepatocarcinogenesis in rats. Int J Cancer,2009,124(3):540-546.
6. Liu C, Russell RM. Nutrition and gastric cancer risk .NutrRev. 2008 ,66(5):237-49.
7. Bartsch H, Pignatelli B, Calmeis S, et al. Inhibition of nitrosation.Basic Life Sci, 1993, 61:27-44.
8. Lowenstein EJ, Daly RJ, Batzer AG, et al. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell ,1992, 70(3): 431-442.
9. Rozakis-Adcock M, Fernley R, Wade J et al. The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1. Nature, 1993, 363(6424): 83-85.
10. Skolnik EY, Batzer A, Li N et al. The function of GRB2 in linking the insulin receptor to Ras signaling pathways. Science, 1993, 260(5116): 1953-1955.
11. Daly RJ, Gu H, Parmar J,et al. The docking protein Gab2 is overexpressed and estrogen regulated in human breast cancer. Oncogene.,2002,21(33):5175-5181.
12. Nguyen DH, Webb DJ, Catling AD,et al. Urokinase-type plasminogen activator stimulates the Ras/Extracellular signal-regulated kinase (ERK) signaling pathway and MCF-7 cell migration by a mechanism that requires focal adhesion kinase, Src, and Shc. Rapid dissociation of GRB2/Sps-She complex is associated with the transient phosphorylation of ERK in urokinase-treated cells. J Biol Chem. 2000 ,275(25):19382-19388.
13. Normanno N, Campiglio M, Maiello MR ,et al.Breast cancer cells with acquired resistance to the EGFR tyrosine kinase inhibitor gefitinib show persistent activation of MAPK signaling. Breast Cancer Res Treat, 2008, 112(1):25-33.
14. Martinez N, García-Domínguez CA, Domingo B, et al. Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction. Cell Signal. 2007, 19(11):2277-2285.
15. Diwan BA, Ramakrishna G, Anderson LM, et al. Overexpression of Grb2 in inflammatory lesions and preneoplastic foci and tumors induced by N-nitrosodimethylamine in Helicobacter hepaticus-infected and -noninfected A/J mice. Toxicol Pathol,2000,28(4):548-54.
16. Li DQ, Tang H, Gui R,et al. Changes in global gene expression induced by NOR1 over expression in HepG2 cells. Prog.Biochem. Biophys,2008,35(4):457-464
17. Dachs GU, Tupper J, Tozer GM. From bench to bedside for gene-directed enzyme prodrug therapy of cancer. Anticancer Drugs. 2005 ,16(4):349-359.
18. Djeha HA, Todryk SM, Pelech S,et al.Antitumor immune responses mediated by adenoviral GDEPT using nitroreductase/CB1954 is enhanced by high-level coexpression of heat shock protein 70. Cancer Gene Ther. 2005, 12(6):560-571.
19. Helsby NA, Ferry DM, Patterson AV,et al. 2-Amino metabolites are key mediators of CB 1954 and SN 23862 bystander effects in nitroreductase GDEPT. Br J Cancer. 2004 ,90(5): 1084-92.
20. Tang MH, Helsby NA, Wilson WR, et al. Aerobic 2and 4-nitroreduction of CB 1954 by human liver. Toxicology, 2005, 216(2-3):129-39
21. Lowenstein EJ, Daly RJ, Batzer AG, et al. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell ,1992, 70(3):431-42.
22. Clark S, Stern M, Horvitz H. C elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains. Nature, 1992,356: 340-344.