The F plasmid transfer activator TraJ is a dimeric helix-turn-helix DNA-binding protein

FEMS Microbiology Letters

Volumn 310, Issue 2, 2010, Pages 112-119

  Rodriguez Maillard, J Manuel ^a   Arutyunov, Denis ^a   Frost, Laura S ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

conjugation; desilencing; F plasmid; H NS; HTH motif; TraJ

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; DNA BINDING PROTEIN; F FACTOR; HELIX LOOP HELIX PROTEIN; HOMODIMER; METHIONINE; MUTANT PROTEIN; OLIGONUCLEOTIDE; TRAJ PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE DELETION; IMMUNOBLOTTING; MATING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN CROSS LINKING; PROTEIN DNA BINDING; PROTEIN FUNCTION; PROTEIN MOTIF; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; TRANSFER OPERON;

BACTERIAL OUTER MEMBRANE PROTEINS; DNA-BINDING PROTEINS; ESCHERICHIA COLI K12; HELIX-TURN-HELIX MOTIFS; SEQUENCE ALIGNMENT; SEQUENCE DELETION;

EID: 77956048953     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02064.x     Document Type: Article

References (26)

1. Achtman M, Willetts N Clark AJ (1971) Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol 106 : 529 538.
2. Aravind L, Anantharaman V, Balaji S, Babu MM Iyer LM (2005) The many faces of the helix-turn-helix domain: transcription regulation and beyond. FEMS Microbiol Rev 29 : 231 262.
3. Ellison DW Miller VL (2006) Regulation of virulence by members of the MarR/SlyA family. Curr Opin Microbiol 9 : 153 159.
4. Fang FC Rimsky S (2008) New insights into transcriptional regulation by H-NS. Curr Opin Microbiol 11 : 113 120.
5. Frost LS Koraimann G (2010) Regulation of bacterial conjugation: balancing opportunity with adversity. Future Microbiol 5 : 1057 1071.
6. Frost LS Manchak J (1998) F- phenocopies: characterization of expression of the F transfer region in stationary phase. Microbiology 144 : 2579 2587.
7. Frost LS, Ippen-Ihler K Skurray RA (1994) Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol Rev 58 : 162 210.
8. * background. J Bacteriol 184 : 5781 5788.
9. Guzman L, Belin MD, Carson MJ Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter. J Bacteriol 177 : 4121 4130.
10. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166 : 557 580.
11. Klimke WA, Rypien CD, Klinger B, Kennedy RA, Rodriguez-Maillard JM Frost LS (2005) The mating pair stabilization protein, TraN, of the F plasmid is an outer-membrane protein with two regions that are important for its function in conjugation. Microbiology 151 : 3527 3540.
12. Lau-Wong IC, Locke T, Ellison MJ, Raivio TL Frost LS (2008) Activation of the Cpx regulon destabilizes the F plasmid transfer activator, TraJ, via the HslVU protease in Escherichia coli. Mol Microbiol 67 : 516 527.
13. Pabo CO Sauer RT (1992) Transcription factors: structural families and principles of recognition. Annu Rev Biochem 61 : 1053 1095.
14. Perez JC, Latifi T Groisman EA (2008) Overcoming H-NS-mediated transcriptional silencing of horizontally acquired genes by the PhoP and SlyA proteins in Salmonella enterica. J Biol Chem 283 : 10773 10783.
15. Silverman PM Sholl A (1996) Effect of traY amber mutations on F-plasmid traY promoter activity in vivo. J Bacteriol 178 : 5787 5789.
16. Silverman PM, Wickersham E Harris R (1991) Regulation of the F plasmid traY promoter in Escherichia coli by host and plasmid factors. J Mol Biol 218 : 119 128.
17. Starcic M, Zgur-Bertok D, Jordi BJ, Wosten MM, Gaastra W van Putten P (2003) The cyclic AMP-cyclic AMP receptor protein complex regulates activity of the traJ promoter of the Escherichia coli conjugative plasmid pRK100. J Bacteriol 185 : 1616 1623.
18. Starcic-Erjavec MJ, van Putten P, Gaastra W, Jordi BJ, Grabnar M Zgur-Bertok D (2003) H-NS and Lrp serve as positive modulators of traJ expression from the Escherichia coli plasmid pRK100. Mol Genet Genomics 270 : 94 102.
19. Strahl-Bolsinger S, Hecht A, Luo K Grunstein M (1997) SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast. Gene Dev 11 : 83 93.
20. Strohmaier H, Noiges R, Kotschan S, Sawers G, Hogenauer G, Zechner EL Koraimann G (1998) Signal transduction and bacterial conjugation: characterization of the role of ArcA in regulating conjugative transfer of the resistance plasmid R1. J Mol Biol 277 : 309 316.
21. Taki K, Abo T Ohtsubo E (1998) Regulatory mechanisms in expression of the traY-I operon of sex factor plasmid R100: involvement of traJ and traY gene products. Genes Cells 3 : 331 345.
22. Tatum EL Lederberg J (1947) Gene recombination in the bacterium Escherichia coli. J Bacteriol 53 : 673 684.
23. Tran HJ, Heroven AK, Winkler L, Spreter T, Beatrix B Dersch P (2005) Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation. J Biol Chem 280 : 42423 42432.
24. Will WR Frost LS (2006) Characterization of the opposing roles of H-NS and TraJ in transcriptional regulation of the F-plasmid tra operon. J Bacteriol 188 : 507 514.
25. Will WR, Lu J Frost LS (2004) The role of H-NS in silencing F transfer gene expression during entry into stationary phase. Mol Microbiol 54 : 769 782.
26. Willetts N (1977) The transcriptional control of fertility in F-like plasmids. J Mol Biol 112 : 141 148.