Designing a long-acting human growth hormone (hGH) by fusing the carboxyl-terminal peptide of human chorionic gonadotropin β-subunit to the coding sequence of hGH

Endocrinology

Volumn 151, Issue 9, 2010, Pages 4410-4417

  Fares, Fuad ^a   Guy, Rachel ^b   Bar Ilan, Ahuva ^b   Felikman, Yana ^b   Fima, Eyal ^b  

^a UNIVERSITY OF HAIFA   (Israel)

^b Weizmann Science Park   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

CHORIONIC GONADOTROPIN BETA SUBUNIT; HUMAN GROWTH HORMONE; HYBRID PROTEIN;

ANIMAL; AREA UNDER THE CURVE; CHEMISTRY; CHO CELL LINE; CRICETULUS; DRUG EFFECTS; GENETICS; HAMSTER; HUMAN; HYPOPHYSECTOMY; MALE; METABOLIC CLEARANCE RATE; METABOLISM; OPEN READING FRAME; RAT; SPRAGUE DAWLEY RAT; TIME; WEIGHT GAIN; WESTERN BLOTTING;

ANIMALS; AREA UNDER CURVE; BLOTTING, WESTERN; CHO CELLS; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; CRICETINAE; CRICETULUS; HUMAN GROWTH HORMONE; HUMANS; HYPOPHYSECTOMY; MALE; METABOLIC CLEARANCE RATE; OPEN READING FRAMES; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS; TIME FACTORS; WEIGHT GAIN;

EID: 77956041902     PISSN: 00137227     EISSN: 00137227     Source Type: Journal    
DOI: 10.1210/en.2009-1431     Document Type: Article

References (38)

1. Altszuler N 1974 Action of growth hormone on carbohydrate metabolism. In: Knobil E, Sawyer WH, eds. Handbook of physiology. Section 7, Vol. 4. Endocrinology. Washington, DC: American Physiology Society; 233-252
2. Ohlsson C, Bengtsson BA, Isaksson OG, Andreassen TT, Slootweg MC 1998 Growth hormone and bone. Endocr Rev 19:55-79
3. Theill LE, Karin M 1993 Transcriptional control of GH expression and anterior pituitary development. Endocr Rev 14:670-689 (Review)
4. de Vos AM, Ultsch M, Kossiakoff AA 1992 Human growth hormone and extracellular domain of its receptor: crystal structure of the complex. Science 255:306-312
5. Abdel-Meguid SS, Shieh HS, Smith WW, Dayringer HE, Violand BN, Bentle LA 1987 Three-dimensional structure of a genetically engineered variant of porcine growth hormone. Proc Natl Acad Sci USA 84:6434-6437
6. Lesniak MA, Roth J, Gorden P, Gavin 3rd JR 1973 Human growth hormone radioreceptor assay using cultured human lymphocytes. Nat New Biol 241:20-22
7. Waters MJ 1999 The GH receptor. In: Kostoyo JL, Goodman HM, eds. Handbook of physiology. Oxford, UK: Oxford University Press; 397-444
8. Carter-Su C, Rui L, Herrington J 2000 Role of the tyrosine kinase JAK2 in signal transduction by growth hormone. Pediatr Nephrol 14:550-557 (Pubitemid 30394427)
9. Raben MS 1958 Treatment of a pituitary dwarf with human growth hormone. J Clin Endocrinol Metab 18:901-903
10. de Boer H, Blok GJ, Van der Veen EA 1995 Clinical aspects of growth hormone deficiency in adults. Endocr Rev 16:63-86
11. Carroll PV, Christ ER, Bengtsson BA, Carlsson L, Christiansen JS, Clemmons D, Hintz R, Ho K, Laron Z, Sizonenko P, Sönksen PH, Tanaka T, Thorne M 1998 Growth hormone deficiency in adulthood and the effects of growth hormone replacement: a review. J Clin Endocrinol Metab 83:382-395
12. Drake WM, Howell SJ, Monson JP, Shalet SM 2001 Optimizing GH therapy in adults and children. Endocr Rev 22:425-450
13. Jørgensen JO, Rubeck KZ, Nielsen TS, Clasen BF, Vendelboe M, Hafstrøm TK, Madsen M, Lund S 2010 Effects of GH in human muscle and fat. Pediatr Nephrol 25:705-709
14. Bright GM, Veldhuis JD, Iranmanesh A, Baumann G, Masheshwari H, Lima J 1999 Appraisal of growth (GH) secretion: evaluation of a composite pharmacokinetic model that discriminates multiple of GH input. J Clin Endocrinol Metab 84:3301-3308
15. Baumann G, Amburn KD, Buchanan TA 1987 The effect of circulating growth hormone binding protein on metabolic clearance, distribution and degradation of human growth hormone. J Clin Endocrinol Metab 64:657-660 (Pubitemid 17044528)
16. Baumann G, Shaw MA, Buchanan TA 1989 In vivo kinetics of a covalent growth hormone-binding protein complex. Metab Clin Exp 38:330-333
17. Zamiri C, Groves MJ 2005 Stabilization of somatropin by heparin. J Pharm Pharmacol 57:555-564
18. Govardhan C, Khalaf N, Jung CW, Simeone B, Higbie A, Qu S, Chemmalil L, Pechenov S, Basu SK, Margolin AL 2005 Novel long-acting crystal formulation of human growth hormone. Pharm Res 22:1461-1470
19. Fares FA, Suganuma N, Nishimori K, LaPolt PS, Hsueh AJ, Boime I 1992 Design of a long-acting follitropin agonist by fusing the C-terminal sequence of the chorionic gonadotropin β subunit to the follitropin β subunit. Proc Natl Acad Sci USA 89:4304-4308
20. LaPolt PS, Nishimori K, Fares FA, Perlas E, Boime I, Hsueh AJW 1992 Enhanced stimulation of follicle maturation and ovulatory potential by long acting follicle-stimulating hormone agonists with extended carboxyl-terminal peptides. Endocrinology 131:2514-2520
21. Furuhashi M, Shikone T, Fares FA, Sugahara T, Hsueh AJ, Boime I 1995 Fusing the carboxy-terminal peptide of the chorionic gonadotropin (CG) β-subunit to the common α-subunit: retention of O-linked glycosylation and enhanced in vivo bioactivity of chimeric human CG. Mol Endocrinol 9:54-63
22. Joshi L, Murata Y, Wondisford FE, Szkudlinski MW, Desai R, Weintraub BD 1995 Recombinant thyrotropin containing a β-subunit chimera with the human chorionic gonadotropin-β carboxy terminus is biologically active, with a prolonged plasma half-life: role of carbohydrate in bioactivity and metabolic clearance. Endocrinology 136:3839-3848
23. Fares F, Ganem S, Hajouj T, Agai E 2007 Development of a long acting erythropoietin by fusing the carboxyl-terminal peptide of human chorionic gonadotropin β subunit to the coding sequence of human erythropoietin. Endocrinology 148:5081-5087
24. 4. Nature 227:680-685
25. Matzuk MM, Hsueh AW, Lapolt P, Tsafriri A, Keene JL, Boime I 1990 The biological role of the carboxyl-terminal extension of human chorionic gonadotropin [corrected] β-subunit. Endocrinology 126:376-383
26. Jentoft N 1990 Why are proteins O-glycosylated? Trends Biochem Sci 15:291-294
27. Fares F 2006 The role of O-linked and N-linked oligosaccharides on the structure-function of glycoprotein hormones: development of agonists and antagonists. Biochim Biophys Acta 1760:560-567
28. Kalyan NK, Bahl OP 1983 Role of carbohydrate inhumanchorionic gonadotropin. Effect of deglycosylation on the subunit interaction and on its in vitro and in vivo biological properties. J Biol Chem 258:67-74
29. Wide L 1986 The regulation of metabolic clearance rate of human FSH in mice by variation of the molecular structure of the hormone. Acta Endocrinol (Copenhagen) 112:336-344 (Pubitemid 16061983)
30. Amin HK, Hunter WM 1970 Human pituitary follicle-stimulating hormone: distribution, plasma clearance and urinary excretion as determined by radioimmunoassay. J Endocrinol 48:307-317
31. Högler W Shaw N 2010 Childhood growth hormone deficiency, bone density, structures and fractures: scrutinizing the evidence. Clin Endocrinol (Oxf) 72:281-289
32. Walsh G 2004 Second-generation biopharmaceuticals. Eur J Pharm Biopharm 58:185-196
33. Clark R, Olson K, Fuh G, Marian M, Mortensen D, Teshima G, Chang S, Chu H, Mukku V, Canova-Davis E, Somers T, Cronin M, Winkler M, Wells JA 1996 long-acting growth hormone produced by conjugation with polyethylene glycol. J Biol Chem 271:21969-21977
34. Cox GN, Rosendahl MS, Chlipala EA, Smith DJ, Carlson SJ, Doherty DH 2007 A long-acting, mono-PEGylated human growth hormone analog is a potent stimulator of weight gain and bone growth in hypophysectomized rats. Endocrinology 148:1590-1597
35. Bouloux PM, Handelsman DJ, Jockenhövel F, Nieschlag E, Rabinovici J, Frasa WL, de Bie JJ, Vootman G, Itskovitz-Eldor J 2001 First human exposure to FSH-CTP in hypogonadotrophic hypogonadal males. Hum Reprod 16:1592-1597
36. Beckers NG, Macklon NS, Devroey P, Platteau P, Boerrigter PJ, Fauser BC 2003 First live birth after ovarian stimulation using a chimeric long-acting human recombinant follicle-stimulating hormone (FSH) agonist (recFSH-CTP) for in vitro fertilization. Fertil Steril 79:621-623 (Pubitemid 36293463)
37. Birken S, Canfield R, Lauer R, Agosto G, Gabel M 1980 Immunochemical determinants unique to human chorionic gonadotropin: importance of sialic acid for antisera generated to the human chorionic gonadotropin beta-subunit COOH-terminal peptide. Endocrinology 106:1659-1664
38. Yakar S, Rosen CJ, Beamer WG, Ackert-Bicknell CL, Wu Y, Liu JL, Ooi GT, Setser J, Frystyk J, Boisclair YR, LeRoith D 2002 Circulating levels of IGF-1 directly regulate bone growth and density. J Clin Invest 110:771-781