Making temperature-sensitive mutants

Methods in Enzymology

Volumn 470, Issue C, 2010, Pages 181-204

  Ben Aroya, Shay ^a   Pan, Xuewen ^b   Boeke, Jef D ^c   Hieter, Philip ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 PROPANOL; AGAR; AMMONIUM ACETATE; AMMONIUM SULFATE; AMPICILLIN; CANAVANINE; CARBENICILLIN; DNA; ENDONUCLEASE; GLUTAMATE SODIUM; ICE; MACROGOL; MESYLIC ACID METHYL ESTER; NITROGEN; PRIMER DNA; ZINC ACETATE;

ALLELE; ARTICLE; BACTERIAL SPORE; BACTERIAL VIABILITY; BACTERIUM COLONY; BACTERIUM TRANSFORMATION; CELL DENSITY; CENTROMERE; CHROMOSOME; DIPLOIDY; DNA FLANKING REGION; DNA MODIFICATION; DNA SEQUENCE; ESCHERICHIA COLI; FUNGAL STRAIN; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE DELETION; GENETIC RECOMBINATION; GENETIC SCREENING; GENETIC TRANSFORMATION; GENOME; HAPLOIDY; HETEROZYGOTE; INOCULATION; INSTRUMENT STERILIZATION; METHODOLOGY; MICROSCOPE; MISSENSE MUTATION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PLASMID; POLYMERASE CHAIN REACTION; PRECIPITATION; PRIORITY JOURNAL; PROMOTER REGION; ROOM TEMPERATURE; SUPERNATANT; TEMPERATURE SENSITIVE MUTANT; WILD TYPE; YEAST CELL;

EID: 77956017412     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(10)70008-2     Document Type: Article

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