In situ protein expression in tumour spheres: Development of an immunostaining protocol for confocal microscopy

BMC Cancer

Volumn 10, Issue , 2010, Pages

  Weiswald, Louis Bastien ^a   Guinebretière, Jean Marc ^b   Richon, Sophie ^a   Bellet, Dominique ^a,b   Saubaméa, Bruno ^c   Dangles Marie, Virginie ^a,d  

^a FACULTÉ DES SCIENCES PHARMACEUTIQUES ET BIOLOGIQUES   (France)

^b INSTITUT CURIE   (France)

^c INSERM   (France)

^d PSL RESEARCH UNIVERSITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDE DEHYDROGENASE; BETA CATENIN; CA 19-9 ANTIGEN; CD133 ANTIGEN; CYTOKERATIN 20; EPITHELIAL CELL ADHESION MOLECULE; HERMES ANTIGEN; MEMBRANE PROTEIN; METHANOL; MONOCLONAL ANTIBODY; PARAFFIN; PARAFORMALDEHYDE; PROTEIN EBP50; TRITON X 100; UNCLASSIFIED DRUG; GLYCOPROTEIN; LEUKOCYTE ANTIGEN; PEPTIDE;

ACCURACY; ANALYTIC METHOD; ANTIBODY LABELING; ANTIGEN EXPRESSION; ARTICLE; CANCER CELL CULTURE; CANCER STEM CELL; CELL COUNT; CELL MEMBRANE; CELL NUCLEUS; CELL SUBPOPULATION; CELL SURFACE; COLORECTAL CANCER; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOPLASM; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; TISSUE FIXATION; TUMOR SPHEROID; TUMOR VOLUME; BIOSYNTHESIS; CYTOLOGY; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; IMAGE PROCESSING; METHODOLOGY; NEOPLASM; PATHOLOGY; TUMOR CELL LINE;

ANTIGENS, CD; CELL LINE, TUMOR; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, NEOPLASTIC; GLYCOPROTEINS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; MICROSCOPY, CONFOCAL; NEOPLASMS; NEOPLASTIC STEM CELLS; PEPTIDES;

EID: 77955983408     PISSN: None     EISSN: 14712407     Source Type: Journal    
DOI: 10.1186/1471-2407-10-106     Document Type: Article

References (52)

1. Debnath J, Brugge JS. Modelling glandular epithelial cancers in three-dimensional cultures. Nat Rev Cancer 2005, 5(9):675-688.
2. Jacks T, Weinberg RA. Taking the study of cancer cell survival to a new dimension. Cell 2002, 111(7):923-925.
3. O'Brien LE, Zegers MM, Mostov KE. Opinion: Building epithelial architecture: insights from three-dimensional culture models. Nat Rev Mol Cell Biol 2002, 3(7):531-537.
4. Smalley KS, Lioni M, Herlyn M. Life isn't flat: taking cancer biology to the next dimension. In Vitro Cell Dev Biol Anim 2006, 42(8-9):242-247.
5. Yamada KM, Clark K. Cell biology: survival in three dimensions. Nature 2002, 419(6909):790-791.
6. Santini MT, Rainaldi G. Three-dimensional spheroid model in tumor biology. Pathobiology 1999, 67(3):148-157.
7. Friedrich J, Ebner R, Kunz-Schughart LA. Experimental anti-tumor therapy in 3-D: spheroids--old hat or new challenge?. Int J Radiat Biol 2007, 83(11-12):849-871.
8. Svendsen CN, ter Borg MG, Armstrong RJ, Rosser AE, Chandran S, Ostenfeld T, Caldwell MA. A new method for the rapid and long term growth of human neural precursor cells. J Neurosci Methods 1998, 85(2):141-152.
9. Singh SK, Clarke ID, Terasaki M, Bonn VE, Hawkins C, Squire J, Dirks PB. Identification of a cancer stem cell in human brain tumors. Cancer Res 2003, 63(18):5821-5828.
10. Farnie G, Clarke RB, Spence K, Pinnock N, Brennan K, Anderson NG, Bundred NJ. Novel cell culture technique for primary ductal carcinoma in situ: role of Notch and epidermal growth factor receptor signaling pathways. J Natl Cancer Inst 2007, 99(8):616-627.
11. Johannsson OT, Staff S, Vallon-Christersson J, Kytola S, Gudjonsson T, Rennstam K, Hedenfalk IA, Adeyinka A, Kjellen E, Wennerberg J, et al. Characterization of a novel breast carcinoma xenograft and cell line derived from a BRCA1 germ-line mutation carrier. Lab Invest 2003, 83(3):387-396.
12. Ricci-Vitiani L, Lombardi DG, Pilozzi E, Biffoni M, Todaro M, Peschle C, De Maria R. Identification and expansion of human colon-cancer-initiating cells. Nature 2007, 445(7123):111-115.
13. Vermeulen L, Todaro M, de Sousa Mello F, Sprick MR, Kemper K, Perez Alea M, Richel DJ, Stassi G, Medema JP. Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity. Proc Natl Acad Sci USA 2008, 105(36):13427-13432.
14. Oh JH, Ku JL, Yoon KA, Kwon HJ, Kim WH, Park HS, Yeo KS, Song SY, Chung JK, Park JG. Establishment and characterization of 12 human colorectal-carcinoma cell lines. Int J Cancer 1999, 81(6):902-910.
15. Toma JG, McKenzie IA, Bagli D, Miller FD. Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells 2005, 23(6):727-737.
16. Dangles V, Validire P, Wertheimer M, Richon S, Bovin C, Zeliszewski D, Vallancien G, Bellet D. Impact of human bladder cancer cell architecture on autologous T-lymphocyte activation. Int J Cancer 2002, 98(1):51-56.
17. Brabletz T, Jung A, Reu S, Porzner M, Hlubek F, Kunz-Schughart LA, Knuechel R, Kirchner T. Variable beta-catenin expression in colorectal cancers indicates tumor progression driven by the tumor environment. Proc Natl Acad Sci USA 2001, 98(18):10356-10361.
18. le Roux L, Volgin A, Maxwell D, Ishihara K, Gelovani J, Schellingerhout D. Optimizing imaging of three-dimensional multicellular tumor spheroids with fluorescent reporter proteins using confocal microscopy. Mol Imaging 2008, 7(5):214-221.
19. Indovina P, Collini M, Chirico G, Santini MT. Three-dimensional cell organization leads to almost immediate HRE activity as demonstrated by molecular imaging of MG-63 spheroids using two-photon excitation microscopy. FEBS Lett 2007, 581(4):719-726.
20. Dangles-Marie V, Pocard M, Richon S, Weiswald LB, Assayag F, Saulnier P, Judde JG, Janneau JL, Auger N, Validire P, et al. Establishment of human colon cancer cell lines from fresh tumors versus xenografts: comparison of success rate and cell line features. Cancer Res 2007, 67(1):398-407.
21. Briffod M, Hacene K, Le Doussal V. Immunohistochemistry on cell blocks from fine-needle cytopunctures of primary breast carcinomas and lymph node metastases. Mod Pathol 2000, 13(8):841-850.
22. Ott SR. Confocal microscopy in large insect brains: zinc-formaldehyde fixation improves synapsin immunostaining and preservation of morphology in whole-mounts. J Neurosci Methods 2008, 172(2):220-230.
23. Dickie R, Bachoo RM, Rupnick MA, Dallabrida SM, Deloid GM, Lai J, Depinho RA, Rogers RA. Three-dimensional visualization of microvessel architecture of whole-mount tissue by confocal microscopy. Microvasc Res 2006, 72(1-2):20-26.
24. Gervaz P, Bucher P, Morel P. Two colons-two cancers: paradigm shift and clinical implications. J Surg Oncol 2004, 88(4):261-266.
25. Bacallao R, Sohrab S, Phillips C. Guiding principles of specimen preservation for confocal fluorescence microscopy. Handbook of biological Confocal Microscopy 2006, 368-380. New York: Springer Science+Business media, Pawley J, 3.
26. Sauer H, Gunther J, Hescheler J, Wartenberg M. Thalidomide inhibits angiogenesis in embryoid bodies by the generation of hydroxyl radicals. Am J Pathol 2000, 156(1):151-158.
27. Melan MA, Sluder G. Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluorescence microscopy. J Cell Sci 1992, 101(Pt 4):731-743.
28. Hannah MJ, Weiss U, Huttner WB. Differential extraction of proteins from paraformaldehyde-fixed cells: lessons from synaptophysin and other membrane proteins. Methods 1998, 16(2):170-181.
29. Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. Subcell Biochem 1998, 31:205-262.
30. Kummar S, Fogarasi M, Canova A, Mota A, Ciesielski T. Cytokeratin 7 and 20 staining for the diagnosis of lung and colorectal adenocarcinoma. Br J Cancer 2002, 86(12):1884-1887.
31. Salces I, Vegh I, Rodriguez-Munoz S, Colina F, Perez A, Soto S, Sanchez F, de la Cruz J, Solis-Herruzo JA. Tissue CA-19.9 content in colorectal adenomas and its value in the assessment of dysplasia. Rev Esp Enferm Dig 2004, 96(4):246-254.
32. Sato T, Nishimura G, Nonomura A, Miwa K, Miyazaki I. Serological studies on CEA, CA 19-9, STn and SLX in colorectal cancer. Hepatogastroenterology 1999, 46(26):914-919.
33. Radtke F, Clevers H, Riccio O. From gut homeostasis to cancer. Curr Mol Med 2006, 6(3):275-289.
34. Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis. Cell 1990, 61(5):759-767.
35. Horst D, Reu S, Kriegl L, Engel J, Kirchner T, Jung A. The intratumoral distribution of nuclear beta-catenin is a prognostic marker in colon cancer. Cancer 2009, 115(10):2063-2070.
36. Horst D, Kriegl L, Engel J, Jung A, Kirchner T. CD133 and nuclear beta-catenin: the marker combination to detect high risk cases of low stage colorectal cancer. Eur J Cancer 2009, 45(11):2034-2040.
37. Chu P, Clanton DJ, Snipas TS, Lee J, Mitchell E, Nguyen ML, Hare E, Peach RJ. Characterization of a subpopulation of colon cancer cells with stem cell-like properties. Int J Cancer 2009, 124(6):1312-1321.
38. Dalerba P, Dylla SJ, Park IK, Liu R, Wang X, Cho RW, Hoey T, Gurney A, Huang EH, Simeone DM, et al. Phenotypic characterization of human colorectal cancer stem cells. Proc Natl Acad Sci USA 2007, 104(24):10158-10163.
39. Du L, Wang H, He L, Zhang J, Ni B, Wang X, Jin H, Cahuzac N, Mehrpour M, Lu Y, et al. CD44 is of functional importance for colorectal cancer stem cells. Clin Cancer Res 2008, 14(21):6751-6760.
40. Dylla SJ, Beviglia L, Park IK, Chartier C, Raval J, Ngan L, Pickell K, Aguilar J, Lazetic S, Smith-Berdan S, et al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS One 2008, 3(6):e2428.
41. Reeder JA, Gotley DC, Walsh MD, Fawcett J, Antalis TM. Expression of antisense CD44 variant 6 inhibits colorectal tumor metastasis and tumor growth in a wound environment. Cancer Res 1998, 58(16):3719-3726.
42. Charafe-Jauffret E, Ginestier C, Iovino F, Tarpin C, Diebel M, Esterni B, Houvenaeghel G, Extra JM, Bertucci F, Jacquemier J, et al. Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer. Clin Cancer Res 16(1):45-55.
43. Tanei T, Morimoto K, Shimazu K, Kim SJ, Tanji Y, Taguchi T, Tamaki Y, Noguchi S. Association of breast cancer stem cells identified by aldehyde dehydrogenase 1 expression with resistance to sequential Paclitaxel and epirubicin-based chemotherapy for breast cancers. Clin Cancer Res 2009, 15(12):4234-4241.
44. Huang EH, Hynes MJ, Zhang T, Ginestier C, Dontu G, Appelman H, Fields JZ, Wicha MS, Boman BM. Aldehyde dehydrogenase 1 is a marker for normal and malignant human colonic stem cells (SC) and tracks SC overpopulation during colon tumorigenesis. Cancer Res 2009, 69(8):3382-3389.
45. Carpentino JE, Hynes MJ, Appelman HD, Zheng T, Steindler DA, Scott EW, Huang EH. Aldehyde dehydrogenase-expressing colon stem cells contribute to tumorigenesis in the transition from colitis to cancer. Cancer Res 2009, 69(20):8208-8215.
46. Taylor I. Liver metastases from colorectal cancer: lessons from past and present clinical studies. Br J Surg 1996, 83(4):456-460.
47. O'Brien CA, Pollett A, Gallinger S, Dick JE. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature 2007, 445(7123):106-110.
48. LaBarge MA, Bissell MJ. Is CD133 a marker of metastatic colon cancer stem cells?. J Clin Invest 2008, 118(6):2021-2024.
49. Shmelkov SV, Butler JM, Hooper AT, Hormigo A, Kushner J, Milde T, St Clair R, Baljevic M, White I, Jin DK, et al. CD133 expression is not restricted to stem cells, and both CD133+ and CD133- metastatic colon cancer cells initiate tumors. J Clin Invest 2008, 118(6):2111-2120.
50. Dallas NA, Xia L, Fan F, Gray MJ, Gaur P, van Buren G, Samuel S, Kim MP, Lim SJ, Ellis LM. Chemoresistant colorectal cancer cells, the cancer stem cell phenotype, and increased sensitivity to insulin-like growth factor-I receptor inhibition. Cancer Res 2009, 69(5):1951-1957.
51. Morales FC, Takahashi Y, Kreimann EL, Georgescu MM. Ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 organizes ERM proteins at the apical membrane of polarized epithelia. Proc Natl Acad Sci USA 2004, 101(51):17705-17710.
52. Horst D, Kriegl L, Engel J, Kirchner T, Jung A. CD133 expression is an independent prognostic marker for low survival in colorectal cancer. Br J Cancer 2008, 99(8):1285-1289.