Differential extraction of proteins from paraformaldehyde-fixed cells: Lessons from synaptophysin and other membrane proteins

Methods: A Companion to Methods in Enzymology

Volumn 16, Issue 2, 1998, Pages 170-181

  Hannah, M J ^a   Weiss, U ^a   Huttner, W B ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOPLASM PROTEIN; DIGITONIN; MEMBRANE PROTEIN; PARAFORMALDEHYDE; PROTEIN; SYNAPTOPHYSIN; TRITON X 100;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL STRAIN; CELLULAR DISTRIBUTION; CONTROLLED STUDY; IMMUNOFLUORESCENCE MICROSCOPY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; RAT; TISSUE FIXATION;

EID: 0031725221     PISSN: 10462023     EISSN: None     Source Type: Journal    
DOI: 10.1006/meth.1998.0664     Document Type: Article

References (32)

1. Griffiths, G. (1993) Fine Structure Immunocytochemistry, Springer, Heidelberg.
2. Goldenthal, K. L., Hedman, K., Chen, J. W., August, J. T., and Willingham, M. C. (1985) J. Histochem. Cytochem. 33, 813-820.
3. Melan, M. A., and Sluder, G. (1992) J. Cell Sci. 101, 731-743.
4. Friedman, S. J., Dewar, C. L., and Skehan, P. (1986) Biochem. Cell Biol. 64, 1326-1332.
5. Griffiths, G., Parton, R. G., Lucocq, J., van Deurs, B., Brown, D., Slot, J. W., and Geuze, H. J. (1993) Trends Cell Biol. 3, 214-219.
6. Tooze, S. A., and Huttner, W. B. (1990) Cell 60, 837-847.
7. Wiedenmann, B., and Franke, W. W. (1985) Cell 41, 1017-1028.
8. Nishiki, T., Kamata, Y., Nemoto, Y., Omori, A., Ito, T., Takahashi, M., and Kozaki, S. (1994) J. Biol. Chem. 269, 10498-10503.
9. Buckley, K., and Kelly, R. B. (1985) J. Cell Biol. 100, 1284-1294.
10. White, S., Miller, K., Hopkins, C., and Trowbridge, I. S. (1992) Biochim. Biophys. Acta 1136, 28-34.
11. Jefferies, W. A., Brandon, M. R., Williams, A. F., and Hunt, S. V. (1985) Immunology 54, 333-341.
12. Rosa, P., Weiss, U., Pepperkok, R., Ansorge, W., Niehrs, C., Stelzer, E. H. K., and Huttner, W. B. (1989) J. Cell Biol. 109, 17-34.
13. Rosa, P., Bassetti, M., Weiss, U., and Huttner, W. B. (1992) J. Histochem. Cytochem. 40, 523-533.
14. Kroemer, A., Glombik, M. M., Huttner, W. B., and Gerdes, H.-H. (1998). J. Cell Biol. 140, 1331-1346.
15. Brose, N., Petrenko, A. G., Südhof, T. C., and Jahn, R. (1992) Science 256, 1021-1025.
16. Mundigl, O., Matteoli, M., Daniell, L., Thomas-Reetz, A., Metcalf, A., Jahn, R., and De Camilli, P. (1993) J. Cell Biol. 122, 1207-1221.
17. Liu, Y., Peter, D., Roghani, A., Schuldiner, S., Privé, G. G., Eisenberg, D., Brecha, N., Edwards, R. H. (1992) Cell 70, 539-551.
18. Kreis, T. E. (1986) EMBO J. 5, 931-941.
19. Brand, S. H., Laurie, S. M., Mixon, M. B., and Castle, J. D. (1991) J. Biol. Chem. 266, 18949-18957.
20. Huovila, A.-P. J., Eder, A. M., and Fuller, S. D. (1992) J. Cell Biol. 118, 1305-1320.
21. Horn, M., and Banting, G. (1994) Biochem. J. 301, 69-73.
22. Wilde, A., Reaves, B., and Banting, G. (1992) FEBS Lett. 313, 235-238.
23. Yeagle, P. L. (1985) Biochim. Biophys. Acta 822, 267-287.
24. Ericsson, J., and Dallner, G. (1993) in Subcellular Biochemistry (Borgese, N., and Harris, J. R., Eds.), Vol. 21, pp. 229-272, Plenum, New York.
25. Subramaniam, V. N., Krijnse-Locker, J., Tang, B. L., Ericsson, M., Yusoff, A. R. M., Griffiths, G., and Hong, W. (1995) J. Cell Sci. 108, 2405-2414.
26. Wassler, M., Jonasson, I., Persson, R., and Fries, E. (1987) Biochem. J. 247, 407-415.
27. Cooper, J. A., Loftus, D. J., Frieden, C., Bryan, J., and Elson, E. L. (1988) J. Cell Biol. 106, 1229-1240.
28. Creighton, T. E. (1993) Proteins: Structures and Molecular Properties, Freeman, New York.
29. Johnston, P. A., and Südhof, T. C. (1990) J. Biol. Chem. 265, 8869-8873.
30. Calakos, N., and Scheller, R. H. (1994) J. Biol. Chem. 269, 24534-24537.
31. Wu, T. T., and Castle, J. D. (1997) J. Cell Sci. 110, 1533-1541.
32. Gratzl, M., and Höhne-Zell, B. (1996) FEBS Lett. 394, 109-116.