Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level

PLoS ONE

Volumn 5, Issue 4, 2010, Pages

  Saito, Kenta ^a   Hatsugai, Noriyuki ^a   Horikawa, Kazuki ^a   Kobayashi, Kentaro ^a   Matsu Ura, Toru ^b   Mikoshiba, Katsuhiko ^b   Nagai, Takeharu ^a,c  

^a HOKKAIDO UNIVERSITY   (Japan)

^b RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; CALCIUM BINDING PROTEIN; CALCIUM ION; CALMODULIN; PROTEIN M13; PROTEIN VENUS; RENILLA LUCIFERIN 2 MONOOXYGENASE; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN; BACTERIAL PROTEIN; CALCIUM; DIAGNOSTIC AGENT; M13 PROTEIN (MYOSIN LIGHT CHAIN KINASE); M13 PROTEIN (MYOSIN LIGHT-CHAIN KINASE); MYOSIN LIGHT CHAIN KINASE; PEPTIDE FRAGMENT; PHOTOPROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CALCIUM SIGNALING; CELL SHAPE; CHROMATOPHORE; CONTROLLED STUDY; DIPOLE; DYNAMICS; GENETIC CODE; HUMAN; HUMAN CELL; LUMINESCENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; ARABIDOPSIS; CYTOLOGY; CYTOPHOTOMETRY; ENERGY TRANSFER; GENETIC ENGINEERING; GENETICS; HELA CELL; METHODOLOGY; SPECTROPHOTOMETRY;

RENILLA LUCIFERASE;

ARABIDOPSIS; BACTERIAL PROTEINS; CALCIUM; CALMODULIN; CYTOLOGICAL TECHNIQUES; CYTOPHOTOMETRY; ENERGY TRANSFER; GENETIC ENGINEERING; HELA CELLS; HUMANS; LUCIFERASES, RENILLA; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; MYOSIN-LIGHT-CHAIN KINASE; PEPTIDE FRAGMENTS; SPECTROPHOTOMETRY;

EID: 77955947906     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0009935     Document Type: Article

References (21)

1. Hoshino H, Nakajima Y, Ohmiya Y (2007) Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging. Nat Methods 4: 637-639.
2. Loening AM, Fenn TD, Wu AM, Gambhir SS (2006) Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. Protein Eng Des Sel 19: 391-400.
3. De A, Ray P, Loening AM, Gambhir SS (2009) BRET3: a red-shifted bioluminescence resonance energy transfer (BRET)-based integrated platform for imaging protein-protein interactions from single live cells and living animals. FASEB J 23: 2702-2709.
4. 2++]C). J Biol Chem 270: 9896-9903.
5. 2++ reporters at the single-cell level. Proc Natl Acad Sci 97: 7260-7265.
6. Shimomura O, Kishi Y, Inouye S (1993) The relative rate of aequorin regeneration from apoaequorin and coelenterazine analogs. Biochem J 296: 549-551.
7. Shimomura O (2006) The jellyfish Aequoria and other luminous coelenterates. In: Bioluminescence. Singapore: World Scientific. pp 115-116.
8. 2++ based on split Renilla luciferase complementation in living cells. Anal Sci 24: 1405-1408.
9. Kim SB, Sato M, Tao H (2009) Split Gaussia luciferase-based bioluminescence template for tracing protein dynamics in living cells. Anal Chem 81: 67-74.
10. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, et al. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20: 87-90.
11. 2++ based on green fluorescent proteins and calmodulin. Nature 388: 882-887.
12. 2++ by circularly permuted yellow fluorescent proteins. Proc Natl Acad Sci 101: 10554-10559.
13. Dacres H, Dumancic MM, Horne I, Trowell SC (2009) Direct comparison of bioluminescence-based resonance energy transfer methods for monitoring of proteolytic cleavage. Anal Biochem 385: 194-202.
14. 2++ probe composed of a single green fluorescent protein. Nat Biotechnol 19: 137-141.
15. Lecourieux D, Ranjeva R, Pugin A (2006) Calcium in plant defence-signalling pathways. New Phytologist 171: 249-269.
16. Mackey D, Holt III BF, Wiig A, Dangl JL (2002) RIN4 interacts with Pseudomonas syringae type III effector molecules and is required for RPM1- mediated resistance in Arabidopsis. Cell 108: 743-754.
17. Grant M, Brown I, Adams S, Knight M, Ainslie A, et al. (2000) The PRM1 plant disease resistance gene facilitates a rapid and sustained increase in cytosolic calcium that is necessary for the oxidative burst and hypersensitive cell death. The Plant J 23: 441-450.
18. Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, et al. (2007) Multimodal fast optical interrogation of neural circuitry. Nature 446: 633-636.
19. Frommer WB, Davidson MW, Campbell RE (2009) Genetically encoded biosensors based on engineered fluorescent proteins. Chem Soc Rev 38: 2833-2841.
20. 2++ with Quin2. Methods Enzymol 172: 230-262.
21. Katagiri F, Thilmony R, He SY (2002) The Arabidopsis thaliana-Pseudomonas syringae interaction. In: Somerville C, Meyerowitz E, eds. The Arabidopsis Book. Boston: American Society for Plant Biology. pp 1-35.