Multiply-primed rolling-circle amplification (MPRCA) of PCV2 genomes: Applications on detection, sequencing and virus isolation

Research in Veterinary Science

Volumn 88, Issue 3, 2010, Pages 436-440

  Dezen, Diogenes ^a   Rijsewijk, Franciscus Antonius Maria ^b   Teixeira, Thais Fumaco ^a   Holz, Carine Lidiane ^c   Cibulski, Samuel Paulo ^a   Franco, Ana Cláudia ^b   Dellagostin, Odir Antonio ^d   Roehe, Paulo M ^a,b  

^a Instituto de Pesquisas Veterinarias Desiderio Finamor IPVDF   (Brazil)

^b FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^c CIRAD   (France)

^d FEDERAL UNIVERSITY OF PELOTAS   (Brazil)

Author keywords

Infectious copy; PMWS; Porcine circovirus type 2; Rolling circle amplification; Swine

Indexed keywords

CAPSID PROTEIN; VIRUS DNA; PRIMER DNA;

ARTICLE; CIRCOVIRUS; CONTROLLED STUDY; GENE AMPLIFICATION; MOLECULAR CLONING; MOLECULAR PHYLOGENY; MULTIPLY PRIMED ROLLING CIRCLE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PORCINE CIRCOVIRUS TYPE 2; POSTWEANING MULTISYSTEMIC WASTING SYNDROME; RESTRICTION MAPPING; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SWINE; VIRUS DETECTION; VIRUS GENOME; VIRUS ISOLATION; ANIMAL; ANIMAL DISEASE; CHEMISTRY; CIRCOVIRIDAE INFECTION; GENETIC TRANSFECTION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; SWINE DISEASE; VIROLOGY;

MIRIDAE; PORCINE CIRCOVIRUS 2; PROKARYOTA; SUIDAE;

ANIMALS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; CLONING, MOLECULAR; DNA PRIMERS; DNA, VIRAL; GENE AMPLIFICATION; GENOME, VIRAL; POLYMERASE CHAIN REACTION; SWINE; SWINE DISEASES; TRANSFECTION;

EID: 77955908211     PISSN: 00345288     EISSN: 15322661     Source Type: Journal    
DOI: 10.1016/j.rvsc.2009.10.006     Document Type: Article

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