An optimized and validated RP-HPLC/UV detection method for simultaneous determination of all-trans-Retinol (Vitamin A) and α-Tocopherol (Vitamin E) in human serum: Comparison of different particulate reversed-phase HPLC columns

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 878, Issue 25, 2010, Pages 2339-2347

  Khan, Abad ^a   Khan, Muhammad I ^a   Iqbal, Zafar ^a   Shah, Yasar ^a   Ahmad, Lateef ^a   Watson, David G ^b  

^a UNIVERSITY OF PESHAWAR   (Pakistan)

^b UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

Tocopherol; All trans retinol; Chromatographic columns; Isocratic mode; Resolution; Validation

Indexed keywords

ALL-TRANS-RETINOL; CHROMATOGRAPHIC COLUMN; ISOCRATIC MODES; RESOLUTION; VALIDATION;

BODY FLUIDS; DICHLOROMETHANE; ETHANOL; HEXANE; METHANOL; OPTIMIZATION; SOLVENT EXTRACTION; TOLUENE;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ALCOHOL; ALPHA TOCOPHEROL; METHANOL; RETINOL; RETINOL ACETATE;

ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; HUMAN; INTERMETHOD COMPARISON; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY ANALYSIS; ULTRAVIOLET RADIATION; VALIDATION PROCESS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; HUMANS; METHANOL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TEMPERATURE; VITAMIN A; VITAMIN E;

EID: 77955843780     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2010.07.009     Document Type: Article

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