Assessment of sample handling practices on microbial activity in sputum samples from patients with cystic fibrosis

Letters in Applied Microbiology

Volumn 51, Issue 3, 2010, Pages 272-277

  Nelson, A ^a   De Soyza, A ^b   Bourke, S J ^c   Perry, J D ^d   Cummings, S P ^a  

^a NORTHUMBRIA UNIVERSITY   (United Kingdom)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c ROYAL VICTORIA INFIRMARY   (United Kingdom)

^d FREEMAN HOSPITAL   (United Kingdom)

Author keywords

DGGE (denaturing gradient gel electrophoresis); fungi; Pseudomonads

Indexed keywords

BACTERIOLOGY; FUNGI; GELS; POLYMERASE CHAIN REACTION;

BACTERIAL COMMUNITY; CYSTIC FIBROSIS; DENATURING GRADIENT GEL ELECTROPHORESIS; EUBACTERIA; FUNGAL COMMUNITY; HAEMOPHILUS INFLUENZAE; MICROBIAL ACTIVITIES; MICROBIAL COMMUNITIES; MICROBIOTAS; MOLECULAR TECHNIQUES; PCR-DGGE; PSEUDOMONADS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS SPP; ROOM TEMPERATURE; SAMPLE HANDLING; SAMPLE STORAGE; SPUTUM SAMPLES;

ELECTROPHORESIS;

MESSENGER RNA;

ASSESSMENT METHOD; BACTERIAL DISEASE; ELECTROKINESIS; FUNGAL DISEASE; GEL; GENETIC ANALYSIS; METABOLISM; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; TEMPERATURE EFFECT;

ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; DENATURING GRADIENT GEL ELECTROPHORESIS; FUNGAL COMMUNITY; GENE TARGETING; HAEMOPHILUS INFLUENZAE; HUMAN; MICROBIAL ACTIVITY; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PSEUDOMONAS; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; ROOM TEMPERATURE; SAMPLING; SPUTUM;

BACTERIA (MICROORGANISMS); FUNGI; HAEMOPHILUS INFLUENZAE; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 77955820014     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2010.02891.x     Document Type: Article

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